بهینه سازی عصاره کاکل ذرت نانو ریزپوشانی شده به روش سطح پاسخ و ارزیابی خواص ریزپوشینه های حاوی عصاره پایدار شده با صمغ دانه ریحان و ایزوله پروتئین کنجد

نویسندگان
ذاکر آقاکشی پور 1
زینب رفتنی امیری 2
رضا اسماعیل زاده کناری 2
1 دانشجوی دکتری دانشگاه علوم کشاورزی و منابع طبیعی ساری
2 دانشگاه علوم کشاورزی و منابع طبیعی ساری
10.22034/FSCT.21.149.71
چکیده
در این مطالعه از صمغ دانه ریحان(BSG) بصورت منفرد و در ترکیب با ایزوله پروتئین کنجد (SPI) به نسبت های 1:0 و 1:1 به منظور انکپسوله کردن عصاره کاکل ذرت(CTE) با استفاده از روش سطح پاسخ در قالب طرح مرکب مرکزی استفاده شد. همچنین به منظور بهینه سازی و بررسی اثر متغییرهای غلظت عصاره کاکل و زمان هموژنیزاسیون بر پاسخ اندازه ذرات و راندمان از معادله درجه دوم استفاده شد. در ابتدا محتوای فنلی کل(TPC)، محتوای فلاونوئید کل(TFC)، فعالیت آنتی اکسیدانی و فعالیت ضد میکروبی عصاره کاکل ذرت بررسی شد. علاوه بر این، فعالیت آنتی اکسیدانی (DPPH & FRAP) اندازه گیری شده، پتانسیل بسیار بالایی از این ترکیبات)11/288FRAP= ، 05/85(DPPH= را برای انکپسوله کردن نشان داد. محتوای فنل کل و فلاونویید حاصل از نتایج آزمون به ترتیب 23/1968 و 20/1346 بود. نتایج حاصل از آنالیز نشان داد نانو ذرات حاصل از ریزپوشانی توسط صمغ دانه ریحان کمترین اندازه ذرات (nm4/494) را داشتند. همچنین بیشترین کارایی درون پوشانی(2/76 درصد) نیز متعلق به نمونه ریزپوشانی شده با صمغ دانه ریحان بود. مقدار پتانسیل زتا در استفاده از پوشش ترکیبی افزایش(57/44) یافت و به بالاترین مقدار خود رسید. در طی نگهداری نانوامولسیون های ریزپوشانی در بازه زمانی صفر، 21،15،7،3 روز، با گذشت زمان پایداری نانوامولسیون ها کاهش یافت به نحوی که بالاترین پایداری مربوط به عصاره نانو ریزپوشانی شده در پوشش ترکیبی(85/94 برای روز صفر و8/33 برای روز 21) را به خود اختصاص داد. نتایج این مطالعه نشان داد که نانو ریزپوشانی عصاره کاکل ذرت با صمغ دانه ریحان کارایی بیشتری از پوشش ترکیبی دارد اما با بررسی سایر پارامترها مانند پایداری، پیشنهاد می گردد این صمغ در ترکیب با سایر مواد دیواره از جمله ایزوله پروتئین کنجد مورد استفاده قرار گیرد تا نتایج مطلوب حاصل شود.

کلیدواژه‌ها
عصاره کاکل
ریزپوشانی
اندازه ذرات
مواد دیواره
بهینه سازی

موضوعات

عنوان مقاله English

Optimization of Nano encapsulated corn tassel extract by response surface methodology and evaluation of the properties of Nanoencapsules containing extract stabilized by basil seed gum and combination of basil seed gum and sesame protein isolate

نویسندگان English

Zaker Aghakeshipour 1
Zeynab Raftani Amiri 2
Reza Esmaeilzadeh kenari 2
1 Sari Agricultural Sciences and Natural Resources University
2 Sari Agricultural Sciences and Natural Resources University
چکیده English

In this study, basil seed gum (BSG) alone, and in combination with sesame protein isolate (SPI) were used in ratios 1:0 and 1:1 in order to encapsulate corn tassel extract (CTE) using the response surface method in the central composite design template was used. Also, the quadratic equation was used to optimize and investigate the effect of the concentration of corn tassel extract and homogenization time on the response of particle size and efficiency. At first, total phenolic content (TPC), antioxidant activity and antibacterial activity of corn tassel extract were investigated. In addition, the measured antioxidant activity (DPPH&FRAP) showed a very high potential of these compounds (FRAP=288.11, DPPH=85.05) for encapsulation. The content of total phenol and flavonoids obtained from the test results were 1968.23 and 1346.20, respectively. The analysis results showed that the nanoparticles obtained from coating basil seed gum had the lowest particle size (494.4nm). Also, the highest coating efficiency (76.2%) belongs to the sample coated with basil seed gum. The value of zeta potential increased (+44.57) in the use of composite coating, and reached its highest value. During the storage of encapsulated Nano emulsion in the period of 0,3,7,15 and 21 days, the stability of nanoemulsions decreased with the passage of time, in such a way that, the highest stability related to encapsulated Nano extract in the combined coating (94.85 per day zero and 33.8 for day 21). The result of this study showed that nanoencapsulated of corn tassel extract with basil seed gum is more effective than the combined coating. But by checking other parameters (such as stability), It is suggested to use this gum in combination with other wall materials, including sesame protein isolates, to achieve the desired results.

کلیدواژه‌ها English

Tassel extract
encapsulate
particle size
wall material
Optimization
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