استخراج ترکیبات زیست‌فعال گلنار فارسی (punica pranatum) با استفاده از روش‌های اهمیک، فراصوت و پرکولاسیون

نویسندگان
1 گروه مهندسی علوم و صنایع غذایی، دانشکده مهندسی صنایع و مکانیک، واحد قزوین، دانشگاه آزاد اسلامی، قزوین، ایران
2 گروه مهندسی علوم و صنایع غذایی، واحد قزوین، دانشگاه آزاد اسلامی، قزوین، ایران
3 دانشکده کشاورزی، دانشگاه ترانس فورمنتیشن، فرانسه
چکیده
ترکیبات زیست‌فعال گیاهی، دارای اثرات سلامت‌بخش برای انسان‌ها و دارای اثرات نگهدارندگی در محصولات غذایی می‌باشند. شرایط استخراج عصاره‌ها تأثیر قابل توجهی بر میزان ترکیبات زیست‌فعال آن‌ها دارد از روش‌های متداول و هم روش‌های جدیدتر برای استخراج ترکیبات زیست‌فعال از گیاهان استفاده می‌شود. هدف از این تحقیق، مقایسه روش‌های استخراج ترکیبات زیست‌فعال گلنار فارسی بود. در این تحقیق از سه روش اهمیک (دماهای 45 و 60 درجه سانتی‌گراد و زمان‌های 40 و 60 دقیقه)، اولتراسونیک (دماهای 40، 50 و 60 درجه سانتی‌گراد و زمان‌های 20 و 40 دقیقه) و پرکولاسیون (زمان‌های 24، 48 و 72 ساعت) برای استخراج عصاره گلنار فارسی استفاده شد و بر اساس بازده استخراج، محتوای فنول کل، آنتوسیانین کل و فعالیت آنتی اکسیدانی (مهار رادیکال DPPH) بهترین شرایط استخراج انتخاب گردید. نتایج نشان داد که بین بازده استخراج هر سه روش اختلاف معنی‌داری وجود نداشت (05/0<p). روش‌ اهمیک دارای محتوای فنول مشابهی با روش اولتراسونیک بود، ولی محتوای آنتوسیانین کل و فعالیت آنتی اکسیدانی بالاتری نشان داد. تیمار بهینه شامل روش اهمیک، دمای 60 درجه سانتی‌گراد و زمان استخراج 40 دقیقه بود و عصاره حاصله تحت این شرایط حاوی mg GAE/g 74/109 فنول کل، mg/g 26/373 آنتوسیانین کل و 11/81 درصد فعالیت آنتی اکسیدانی بود. از عصاره گلنار فارسی در صنایع غذایی و دارویی می‌توان استفاده کرد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Extraction of bioactive compounds of Persian Golnar (punica pranatum) using ohmic, ultrasound and perculation methods

نویسندگان English

Nazanin Amir ahmadi 1
Akram Sharifi 2
Mohamed Koubaa 3
1 Department of Food Science and Technology, Qazvin Branch, Islamic Azad University, Qazvin, Iran.
2 Department of Food Science and Technology, Qazvin Branch, Islamic Azad University, Qazvin, Iran.
3 Unité Transformations Intégrées de la Matière Renouvelable TIMR and Ecole Supérieure de Chimie Organique et Minérale ESCOM, Université de Technologie de Compiègne, Compiègne, France
چکیده English

Herbal bioactive compounds are secondary metabolites of plants that are produced in response to environmental stress and to protect the plant against harsh conditions. These compounds have both health effects on humans and also have preservative effects in food products. The conditions for extracting active compounds have a significant effect on their functional activities, and conventional and new methods have been used to extract bioactive compounds from plants. The aim of this study was to compare the extraction methods of bioactive compounds of Persian Golnar extract (PGE). In this research three methods of ohmic (temperatures of 45 °C and 60 °C for 40 and 60 min), ultrasonic (temperatures of 40 °C, 50 °C and 60 °C for 20 and 40 min) and perculation (for 24, 48 and 72 h) were used to prepare PGE, and the best extract was selected based on the extraction yield, total phenol content (TPC), total anthocyanin content (TAC) and antioxidant activity (DPPH radical scavenging). The results showed that there was no significant difference between the extraction yields of all three methods (p>0.05). The ohmic method had the same TPC as the ultrasonic method, but showed a higher TAC and antioxidant activity and was chosen as the best method. The optimal treatment in this stage included ohmic method, 60 °C and 40 min, and the extract obtained under these conditions contained 109.74 mg GAE/g of TPC, 373.26 mg/g of TAC and 81.11% antioxidant activity.

کلیدواژه‌ها English

Punica pranatum
Extraction Methods
phenolic compounds
Antioxidant activity
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