تعیین فنول و فلاونوئید کل، پتانسیل آنتی‌اکسیدانی و فعالیت ضدمیکروبی عصاره هیدروالکلی پنیرباد بر قارچ‌های عامل فساد پس از برداشت میوه سیب و توت فرنگی

نویسندگان
مصطفی رحمتی جنیدآباد 1
فاطمه برنا 1
مختار حیدری 2
1 استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 دانشیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.22034/FSCT.20.142.171
چکیده
در پژوهش حاضر، عصاره هیدروالکلی پنیرباد (Withania somnifera) استخراج گردید و سپس محتوای فنول کل (به روش معرف فولین-سیوکالتو)، فلاونوئید کل (به روش رنگ‌سنجی کلرید آلومینیوم)، فعالیت آنتی‌اکسیدانی (بر پایه روش‌های مهار رادیکال آزاد DPPH و ABTS) و اثر ضدقارچی آن بر ریزوپوس استولونیفر، بوتریتیس سینرا، پنی‌سیلیوم اکسپانسوم و آلترناریا آلترناتا (قارچ­های عامل فساد پس از برداشت میوه سیب و توت فرنگی) بر اساس روش‌های دیسک دیفیوژن آگار، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی تعیین گردید. میزان فنول و فلاونوئید کل عصاره پنیرباد به ترتیب برابر با mg GAE/g 16/53 و mg QE/g 20/28 بدست آمد. قدرت آنتی‌اکسیدانی عصاره هیدروالکلی بر حسب درصد مهار رادیکال‌های آزاد DPPH و ABTS به ترتیب برابر با μg/ml 80/69 و 15/57 بود. نتایج فعالیت ضدقارچی بر اساس روش‌های دیسک دیفیوژن آگار و چاهک آگار نشان داد که افزایش غلظت عصاره سبب افزایش معنی‌دار قطر هاله عدم رشد گردید. بوتریتیس سینرا و آلترناریا آلترناتا به ترتیب مقاوم‌ترین و حساس‌ترین سویه‌های قارچی در برابر عصاره بودند. بطور کلی پنی‌سیلیوم اکسپانسوم و آلترناریا آلترناتا نسبت به ریزوپوس استولونیفر و بوتریتیس سینرا حساس‌تر بودند، بطوریکه حداقل غلظت مهارکنندگی و کشندگی برای این گروه به ترتیب mg/ml 16 و 128 مشاهده گردید.
کلیدواژه‌ها
پنیرباد
فعالیت ضدقارچی
اثر آنتی‌اکسیدانی
عصاره زیست فعال

موضوعات

عنوان مقاله English

Determination of total phenols and flavonoids, antioxidant potential and antimicrobial activity of hydroalcoholic extract of Withania somnifera against fungi cause spoilage postharvest apple and strawberry fruits

نویسندگان English

Mostafa Rahmati 1
Fatemeh Borna 1
Mokhtar Heidari 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

In the present study, the hydroalcoholic extract of Withania somnifera was extracted and then the content of total phenol (by Folin-Ciocalteu reagent), total flavonoid (by aluminum chloride method), antioxidant activity (based on DPPH and ABTS free radical scavenging methods) and its antifungal effect against Rhizopu stolonifers, Botrytis cinerea, Penicillium expansum and Alternaria alternata (fungi cause spoilage postharvest apple and strawberry fruits) were determined based on disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum fungicidal concentration. The amount of phenol and flavonoids in the extract was 53.16 mg GAE/g and 28.20 mg QE/g, respectively. The antioxidant power of the hydroalcoholic extract was 69.80 and 57.15 μg/ml in terms of DPPH and ABTS free radical scavenging, respectively. The results of antimicrobial activity based on disk diffusion agar and well diffusion agar showed that increasing the concentration of the extract caused a significant increase in the diameter of the growth inhibition zone and Botrytis cinerea and Alternaria alternata were the most resistant and sensitive strains to the extract, respectively. In general, Penicillium expansum and Alternaria alternata were more sensitive than Rhizopus stolonifer and Botrytis cinerea with minimum inhibitory and fungicidal concentrations of 16 and 128 mg/ml, respectively.

کلیدواژه‌ها English

Withania somnifera
Antifungal activity
Antioxidant effect
Bioactive extract
[1] D. Patel, R. Kumar, S. Prasad, and S. Hemalatha, "Pedalium murex Linn (Pedaliaceae) fruits: a comparative antioxidant activity of its different fractions," Asian Pacific journal of tropical biomedicine, vol. 1, no. 5, pp. 395-400, 2011.
[2] A. Moure et al., "Natural antioxidants from residual sources," Food chemistry, vol. 72, no. 2, pp. 145-171, 2001.
[3] J. W.-F. Law, N.-S. Ab Mutalib, K.-G. Chan, and L.-H. Lee, "Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations," Frontiers in microbiology, vol. 5, p. 770, 2015.
[4] J. B. L. Tan and Y. Y. Lim, "Critical analysis of current methods for assessing the in vitro antioxidant and antibacterial activity of plant extracts," Food Chemistry, vol. 172, pp. 814-822, 2015.
[5] B. Alizadeh Behbahani, F. Falah, A.Vasiee, & F. Tabatabaee Yazdi, "Control of microbial growth and lipid oxidation in beef using a Lepidium perfoliatum seed mucilage edible coating incorporated with chicory essential oil", Food Science & Nutrition, vol. 9, no. 5, pp. 2458-2467, 2021.
[6] S. Heydari, H.Jooyandeh, B. Alizadeh Behbahani, & M. Noshad, (2020). The impact of Qodume Shirazi seed mucilage‐based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study. Food Science & Nutrition, 8(12), 6497-6512.
[7] H. Tanavar, H. Barzegar, B. Alizadeh Behbahani, & M. A. Mehrnia, (2021). Investigation of the chemical properties of Mentha pulegium essential oil and its application in Ocimum basilicum seed mucilage edible coating for extending the quality and shelf life of veal stored in refrigerator (4° C). Food Science & Nutrition, 9(10), 5600-5615.
[8] A. Alghooneh, B. A. Behbahani, H. Noorbakhsh, and F. T. Yazdi, "Application of intelligent modeling to predict the population dynamics of Pseudomonas aeruginosa in Frankfurter sausage containing Satureja bachtiarica extracts," Microbial pathogenesis, vol. 85, pp. 58-65, 2015.
[9] B. Alizadeh Behbahani, M. Noshad, and F. Falah, "Study of chemical structure, antimicrobial, cytotoxic and mechanism of action of Syzygium aromaticum essential oil on foodborne pathogens," Potravinarstvo, vol. 13, no. 1, pp. 875-883, 2019.
[10] N. J. Dar, A. Hamid, and M. Ahmad, "Pharmacologic overview of Withania somnifera, the Indian Ginseng," Cellular and molecular life sciences, vol. 72, no. 23, pp. 4445-4460, 2015.
[11] R. Udayakumar et al., "Antioxidant effect of dietary supplement Withania somnifera L. reduce blood glucose levels in alloxan-induced diabetic rats," Plant foods for human nutrition, vol. 65, no. 2, pp. 91-98, 2010.
[12] T. Dhanani, S. Shah, N. Gajbhiye, and S. Kumar, "Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera," Arabian Journal of Chemistry, vol. 10, pp. S1193-S1199, 2017.
[13] S. Arora, S. Dhillon, G. Rani, and A. Nagpal, "The in vitro antibacterial/synergistic activities of Withania somnifera extracts," Fitoterapia, vol. 75, no. 3-4, pp. 385-388, 2004.
[14] S. P. Wong, L. P. Leong, and J. H. W. Koh, "Antioxidant activities of aqueous extracts of selected plants," Food chemistry, vol. 99, no. 4, pp. 775-783, 2006.
[15] A. Gedikoğlu, M. Sökmen, and A. Çivit, "Evaluation of Thymus vulgaris and Thymbra spicata essential oils and plant extracts for chemical composition, antioxidant, and antimicrobial properties," Food science & nutrition, vol. 7, no. 5, pp. 1704-1714, 2019.
[16] M. Yeganegi, F. T. Yazdi, S. A. Mortazavi, J. Asili, B. A. Behbahani, and A. Beigbabaei, "Equisetum telmateia extracts: Chemical compositions, antioxidant activity and antimicrobial effect on the growth of some pathogenic strain causing poisoning and infection," Microbial pathogenesis, vol. 116, pp. 62-67, 2018.
[17] M. Noshad, B. Alizadeh Behbahani, H. Jooyandeh, M. Rahmati‐Joneidabad, M. E. Hemmati Kaykha, and M. Ghodsi Sheikhjan, "Utilization of Plantago major seed mucilage containing Citrus limon essential oil as an edible coating to improve shelf‐life of buffalo meat under refrigeration conditions," Food Science & Nutrition, vol. 9, no. 3, pp. 1625-1639, 2021.
[18] F. T. Yazdi and B. A. Behbahani, "Antimicrobial effect of the aqueous and ethanolic Teucrium polium L. extracts on gram positive and gram negative bacteria “in vitro”," Archives of Advances in Biosciences, vol. 4, no. 4, pp. 56-62, 2013.
[19] B. A. Behbahani, F. T. Yazdi, F. Shahidi, H. Noorbakhsh, A. Vasiee, and A. Alghooneh, "Phytochemical analysis and antibacterial activities extracts of mangrove leaf against the growth of some pathogenic bacteria," Microbial pathogenesis, vol. 114, pp. 225-232, 2018.
[20] F. Falah, K. Shirani, A. Vasiee, F. T. Yazdi, and B. A. Behbahani, "In vitro screening of phytochemicals, antioxidant, antimicrobial, and cytotoxic activity of Echinops setifer extract," Biocatalysis and Agricultural Biotechnology, vol. 35, p. 102102, 2021.
[21] H. Tanavar, H. Barzegar, B. Alizadeh Behbahani, and M. A. Mehrnia, "Mentha pulegium essential oil: chemical composition, total phenolic and its cytotoxicity on cell line HT29," Iranian Food Science and Technology Research Journal, vol. 16, no. 5, pp. 643-653, 2020.
[22] F. Shahidi, F. Tabatabaei Yazdi, S. Roshanak, B. Alizadeh Behbahani, A. Vasiee, and N. Norouzi, "Antimicrobial activity of Taraxacum pseudocalocephalum leaves extract on pathogenic microorganisms and comparison with common therapeutic antibiotics in vitro," Iranian Journal of Infectious Diseases and Tropical Medicine, vol. 23, no. 83, pp. 37-46, 2019.
[23] B. Alizadeh Behbahani, F. Shahidi, F. T. Yazdi, and M. Mohebbi, "Antifungal effect of aqueous and ethanolic mangrove plant extract on pathogenic fungus" in vitro"," International Journal of Agronomy and Plant Production, vol. 4, no. 7, pp. 1652-1658, 2013.
[24] Rahmati-Joneidabad, M., Alizade Behbahani, B., & Noshad, M. (2021). Antifungal effect of Satureja khuzestanica essential oil on Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer causing strawberry’s rot and mold. Food Science and Technology, 18(115), 171-180.
[25] Rahmati-Joneidabad, M., and Alizadeh Behbahani, B. 2021. Identification of chemical compounds, antioxidant potential, and antifungal activity of Thymus daenensis essential oil against spoilage fungi causing apple rot. Iranian Food Science and Technology Research 17(5): 691-700.
[26] N. Alam, M. Hossain, M. A. Mottalib, S. A. Sulaiman, S. H. Gan, and M. I. Khalil, "Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities," BMC complementary and alternative medicine, vol. 12, no. 1, pp. 1-8, 2012.
[27] D. Vattem, Y.-T. Lin, R. Labbe, and K. Shetty, "Antimicrobial activity against select food-borne pathogens by phenolic antioxidants enriched in cranberry pomace by solid-state bioprocessing using the food grade fungus Rhizopus oligosporus," Process Biochemistry, vol. 39, no. 12, pp. 1939-1946, 2004.
[28] P. Jain and R. Varshney, "Antimicrobial activity of aqueous and methanolic extracts of Withania somnifera (Ashwagandha)," J Chem Pharm Res, vol. 3, no. 3, pp. 260-263, 2011.
[29] F. Garavand, N. Eghbal, M. Nooshkam, I. Miraballes, and S. M. Jafari, "Salt, spices, and seasonings formulated with nano/microencapsulated ingredients," in Application of Nano/Microencapsulated Ingredients in Food Products: Elsevier, 2021, pp. 435-467.