بررسی ویژگی‌های ساختاری نانولیپوزوم‌های‌ حاوی عصاره جلبک پادینا (Padina distromatic) تولید شده به روش حرارتی

نویسندگان
خدیجه شیرانی بیدآبادی 1
شیلا صفائیان 2
رضوان موسوی ندوشن 2
ناهید رحیمی فرد 3
1 دانشجوی دکترای تخصصی، گروه علوم و صنایع غذایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران.
2 دانشیار گروه علوم و صنایع غذایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران.
3 استاد تمام گروه میکروبیولوژی- اداره کل آزمایشگاه های کنترل غذا و دارو، سازمان غذا و دارو، وزارت بهداشت، درمان و آموزش پزشکی، تهران ایران.
10.22034/FSCT.20.138.107
چکیده
ریزپوشانی ترکیبات زیست فعال در حامل‏های لیپیدی از جمله لیپوزوم­ها علاوه بر بهبود پایداری در زمان نگهداری با افزایش زیست‏دسترس‏پذیری و رهایش کنترل شده موجب ارتقاء کارایی این ترکیبات در شرایط درون‏تنی می­گردد. جلبک پادینا دارای مقادیر قابل توجهی از پلی‏فنل‏ها با خواص آنتی‏اکسیدانی، ضدمیکروبی و مهار آنزیم استیل‏کولین‏استراز (AChE) است که قابلیت کاربرد به عنوان مکمل ضدسرطان و بهبوددهنده اختلالات نورولوژیک را دارد. از این رو هدف از انجام این پژوهش تولید و بررسی خصوصیات ساختاری نانولیپوزوم­های حاوی عصاره جلبک پادینا با استفاده از روش حرارتی بود. بدین منظور آزمون‏های توزیع و اندازه ذرات نانولیپوزوم‏ها، پتانسیل زتا، راندمان درون‏پوشانی عصاره و تصاویر میکروسکوپ الکترونی عبوری انجام شد. نتایج نشان داد اندازه ذرات نانولیپوزوم­های تولیدی در سطوح متفاوت فسفاتیدیل کولین (لسیتین) (5/2 و 5/4 درصد) و عصاره جلبک پادینا (7/0 و 2 درصد) در محدوده 318-60 نانومتر بودند. نتایج آزمون شاخص چند پراکندگی و پتانسیل زتا نیز حاکی از یکنواختی ذرات تولیدی همراه با دافعه الکترواستاتیکی بالای میان ذرات بود. همچنین یافته‏های بدست آمده نشان داد قابلیت بارگذاری ذرات نانولیپوزوم (راندمان درون‏پوشانی عصاره) در کمترین سطح ماده دیواره­ای (5/2 درصد) و بیشترین سطح عصاره جلبک پادینا (2 درصد) 8/52 درصد بود. ارزیابی خصوصیات مورفولوژیک ساختار نانولیپوزوم با استفاده از میکروسکوپ الکترونی عبوری نیز نشان‏دهنده شکل‏گیری ذراتی یکنواخت با هندسه کروی شکل بود. با توجه به نتایج حاصله از این پژوهش می‏توان گفت که قابلیت تولید ساختار نانولیپوزومی حاوی عصاره جلبک پادینا با خصوصیات ساختاری مناسب مهیاست که این امر می­تواند دورنمای کاربرد احتمالی این عصاره با رویکردی درمانی را بهبود ببخشد.
کلیدواژه‌ها
نانولیپوزوم
عصاره جلبک پادینا
ضد سرطان
ضد آلزایمر

موضوعات

عنوان مقاله English

Investigating the structural properties of nanoliposomes containing Padina distromatic algae extract fabricated by heating method

نویسندگان English

Khadijeh Shirani bidabadi 1
shilla safaeian 2
Rezvan Mousavi nadushan 2
Nahid Rahimifard 3
1 Department of Food Science and Technology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
2 Department of Food Science and Technology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3 Food and Drug Control Laboratories, Ministry of Health and Medical Education, Tehran, Iran.
چکیده English

Microencapsulation of bioactive compounds in lipid carriers, such as liposomes, in addition to improving stability during storage by increasing bioavailability and controlled release, increases the efficiency of these compounds in vivo. The studies conducted on Padina algae show the existence of a high level of phenolic and antimicrobial compounds. Also, this alga has a significant amount of polyphenols with antioxidant and anti-AChE (acetylcholinesterase) properties, which can be used as a supplement to improve neurological disorders. Therefore, the purpose of this research was to produce and investigate the structural properties of nanoliposomes containing Padina algae extract using the heating method. The particle size of nanoliposomes produced at varying levels of lecithin and loaded extract was obtained in the range between 318 and 60 nm. The resulting values ​​for the polydispersity index and zeta potential indicate the uniformity of the produced particles along with the high electrostatic repulsion between the particles. The ability to load liposome particles at the lowest level of wall substance and the highest concentration level of the extract reached 52.8±0.3% in this research. Evaluation of the morphological characteristics of the structure using a transmission electron microscope shows the formation of uniform particles with a spherical geometry. The results of this research show the ability to produce a liposome structure containing Padina algae extract with suitable structural properties. These results can improve the prospect of possible use of this extract with a therapeutic approach.

کلیدواژه‌ها English

Nanoliposome
Padina algae extract
Anti-cancer
Anti- alzheimer
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