بهینه‌سازی شرایط استخراج آبی ترکیبات فنلی و آنتی‌اکسیدانی برگ و ریشه گیاه داغ قارپوزی با کاربرد متدولوژی سطح پاسخ

نویسندگان
عبدالواحد صفرزایی 1
reza esmaeilzadeh kenari 2
رضا فرهمندفر 3
1 دانشجوی دکتری، گروه علوم و صنایع غذایی، دانشکده مهندسی زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
2 گروه علوم و صنایع غذایی، دانشکده مهندسی زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
3 دکتری، دانشیار، گروه علوم و صنایع غذایی، دانشکده مهندسی زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
10.22034/FSCT.20.138.26
چکیده
عصاره گیاهان دارویی دارای ترکیبات موثری نظیر فنلی، آنتی‌اکسیدانی و ضدمیکروبی می­باشند. جهت استخراج عصاره از گیاهان می­توان از روش‌های سنتی مانند سوکسله و غرقابی و یا فناوری­های نوین غیر­حرارتی نظیر امواج فراصوت استفاده نمود. نوع روش استخراج بر کمیت و کیفیت عصاره استخراجی تاثیرگذار است. هدف از این مطالعه، بررسی کارایی استفاده از امواج فراصوت در استخراج ترکیبات فنلی و آنتی‌اکسیدانی برگ و ریشه گیاه داغ قارپوزی در محیط آبی بود. روش آماری سطح پاسخ و طرح باکس بنکن به منظور بهینه‌سازی فاکتورهای استخراج شامل زمان در سه سطح (10، 25 و 40 دقیقه) و شدت صوت در سه سطح (40، 70 و 100 درصد) با حلال آبی مورد استفاده قرار گرفتند. نتایج آزمون‌های انجام شده با روش آماری سطح پاسخ نشان داد، شدت صوت به عنوان تاثیرگذارترین فاکتور استخراج ترکیبات فنلی و آنتی‌اکسیدانی از برگ و ریشه گیاه داغ قارپوزی نسبت به زمان فراصوت‌دهی می‌باشد به طوری که با افزایش زمان و شدت صوت میزان استخراج این ترکیبات افزایش یافت. شرایط بهینه استخراج ترکیبات فنلی و آنتی‌اکسیدانی با حمام فراصوت، زمان 36 دقیقه و شدت صوت 91 درصد تعیین گردید. در این شرایط بهینه، میزان ترکیبات فنلی کل برگ و ریشه به ترتیب 80/21 و 96/14 میلی­گرم بر گرم و میزان IC50 برگ و ریشه نیز به ترتیب 75/11 و 17/52 میکروگرم بر میلی‌گرم بدست آمد.
کلیدواژه‌ها
داغ قارپوزی
استخراج
بهینه‌سازی
فراصوت

موضوعات

عنوان مقاله English

Optimization of aqueous extraction conditions of phenolic and antioxidant compounds of Caper (Capparis spinosa) leaves and roots using response surface

نویسندگان English

Abdolvahed Safarzaei1 Abdolvahed Safarzaei 1
reza esmaeilzadeh kenari 2
Reza Farahmandfar 3
1 Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Ph.D, Professor, Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
3 Ph.D, associate Professor, Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده English

Medicinal plant extracts contain effective compounds such as phenolic, antioxidant and antimicrobial. To extract the extracts from plants, traditional methods such as soxhlet and maceration or new nonthermal technologies such as ultrasound can be used. The type of extraction method affects the quantity and quality of the extract. The aim of this study was to evaluate the efficiency of using ultrasound in the aqueous extraction of phenolic and antioxidant compounds of Capparis spinosa leaves and roots. Response surface methodology (RSM) and Box–Behnken design were used to optimize the extraction factors, including extraction time (10, 25, 40 min) and sound intensity (40, 70, 100 %) with aqueous solvent. The results showed sound intensity was more effective factor than time. By increasing of sound intensity and the time of extraction the yield increased. The optimum conditions for extraction of phenolic and antioxidant compounds were as follows: extraction time 36 min and sound intensity 91 percent. Total phenolic content was obtained 21.80 mg/g in leaf and 14.96 mg/g in root and IC50 was obtained 11.75 µg/mg in leaf and 52.17 µg/mg in root.

کلیدواژه‌ها English

Extraction
Optimization
Ultrasound
Capparis spinosa
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