غنی سازی نوشیدنی تخمیری بر پایه گندم سیاه و عدس با نانولیپوزوم های ویتامین E وC

نویسندگان
1 دانشجوی دکتری، گروه علوم و صنایع غذایی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
2 استادیار، گروه علوم و صنایع غذایی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
3 دانشیار، گروه علوم و صنایع غذایی، دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران
4 دانشیار، گروه علوم و صنایع غذایی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
5 استاد، گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران
چکیده
در این تحقیق لیپوزوم‌های بر پایه لیستین به عنوان حامل‌های ویتامینE و C، جهت غنی‌سازی نوشیدنی تخمیری بر پایه عدس/گندم سیاه با روش هیدراسیون تهیه شد. اندازه و شکل ذرات با تفرق نور و میکروسکوپ الکترونی روبشی آنالیز شد. درصد کارایی انکپسولاسیون ویتامین E وC با روش HPLC تعیین شد. کارایی درون پوشانی نانولیپوزوم ویتامین E و C به ترتیب 5/88 و 50 درصد بود. متوسط قطر حجمی نانولیپوزوم ویتامین C کوچکتر از متوسط قطر حجمی نانولیپوزوم ویتامینE بود. نتایج نشان داد لیپوزوم‌های تولید شده به روش سونیکاسیون، نانولیپوزوم‌های پایدار با اندازۀ کوچک، ضریب پخش‏پذیری پایین (3/0) و تک مد و بار سطحی منفی بالا هستند. تصاویر میکروسکوپ الکترونی وجود ذرات کروی با اندازه نانومتری را در نانولیپوزوم‌ها نشان داد. ترکیب فرمولاسیون‌های لیپوزومی و ویتامین‌های آزاد در نوشیدنی تخمیری خصوصیات شیمیایی نوشیدنی شاهد را تغییر نداد و شمارش باکتری‌های پروبیوتیک در این فرمولاسیون‌ها بعد از 15 روز نگهداری در دمای 4 درجه تفاوتی با نمونه شاهد نداشت.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Enrichment of buckwheat and lentil based fermented beverages with vitamin E and vitamin C Nano liposomes

نویسندگان English

Mir hossein musavi 1
Mehdi Gharekhani 2
Kazem Alirezalu 3
Leila Roufegarinejad 4
Sodeif Azadmard_damirchi 5
1 PhD Student, Department of Food Science and Technology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Department of Food Science and Technology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 Department of Food Science and Technology, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran
4 Department of Food Science and Technology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
5 Department of Food Science and Technology, University of Tabriz, Tabriz, Iran
چکیده English

lecithin -based liposomes were studied as carriers of vitamins E and C to enrich lentil/buckwheat-based fermented beverages. Liposomes prepared by hydration method. Particle size and shape were analyzed by light scattering and scanning electron microscopy. The percentage of encapsulation efficiency of vitamins E and C was determined by HPLC method. The encapsulation efficiency of vitamin E and C nanoliposome's was 88.5% and 50%, respectively. The average volume diameter of vitamin C nanoliposomes was smaller than the average volume diameter of vitamin E nanoliposomes. The results showed that the liposomes produced by sonication method are stable nanoliposomes with small size, low Polydispersity index and single-mode with high negative surface charge. Electron Microscopy Images showed nanometer-sized spherical nanoliposomes. The combination of liposomal formulations and free vitamins in the fermented beverage did not change the chemical properties of the control beverage. The count of probiotic bacteria in these formulations after 15 days of storage at 4 ° C didn’t change compare to the control beverage.

کلیدواژه‌ها English

Fermented beverage
Nanoliposome
Vitamin E
Vitamin C
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