تاثیر پوشش ایزوله پروتئین آب پنیر و اسانس روغنی هل بر ماندگاری پنیر سفید

نویسندگان
1 دانشکده کشاورزی و منابع طبیعی، مؤسسه آموزش عالی آفاق
2 دانشگاه علوم پزشکی تبریز
3 دانشکده تغذیه و علوم غذایی، دانشگاه علوم پزشکی تبریز
چکیده
بسته ­بندی پنیر به ­عنوان یکی از فرآوری­ها تأثیر مهمی در افزایش ماندگاری پنیر و کیفیت آن دارد. هدف این مطالعه، بسته­ بندی پنیر سفید ایرانی با استفاده از پوشش خوراکی ایزوله پروتئین آب پنیر حاوی سطوح مختلف اسانس روغنی هل (0، 1، 1/5 و 2 %) در طول مدت نگهداری بود. نمونه ­ها از نظر تغییرات فیزیکوشیمیایی، میکروبی و حسی طی 60 روز نگهداری بررسی شدند. همچنین ترکیبات شیمیایی اسانس روغنی هل توسط دستگاه GC-MS شناسایی شد. در آنالیز ترکیب شیمیایی اسانس روغنی هل، دو ترکیب اصلی شامل α-ترپنیل استات (46/705 %) و 1.8-سینئول (27/415 %) شناسایی شدند. بررسی تغییرات pH نتایج نشان داد که pH تمام نمونه­ها در طی مدت نگهداری به طور معنی داری کاهش یافت (0/05P<). در روز پایان نگهداری پائین­ترین میزان پراکسید در نمونه پنیر پوشش شده با 2% اسانس روغنی هل (meq O2/kg 0/210/97±) بود. نتایج تغییرات شاخص تیوباربیتوریک اسید نشان داد که دو نمونه پنیر سفید پوشش شده با ایزوله پروتئین آب پنیر حاوی 1/5 و 2% اسانس روغنی هل دارای کم­ترین میزان تیوباربیتوریک اسید از روز هفتم تا پایان دوره نگهداری بودند. پائین­ترین جمعیت باکتری کل در روز پایان نگهداری در نمونه پنیر سفید با پوشش حاوی 2% اسانس روغنی هل (Log cfu/g 0/56±431/2) بود. نتایج ارزیابی حسی نشان داد که پوشش خوراکی ایزوله پروتئین آب پنیر حاوی 1/5 % اسانس روغنی هل خصوصیات حسی مطلوب­تری نسبت به سایر پوشش­ ها داشت. با توجه به نتایج حاصل از پژوهش حاضر می­توان بیان کرد که پوشش­ های خوراکی حاوی اسانس­ های گیاهی می ­تواند در بسته­ بندی و پوشش ­دهی پنیر سفید جهت افزایش کیفیت و ایمنی آن استفاده شود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Effect of whey protein isolate coating with cardamom essential oil on shelf life of white cheese

نویسندگان English

Sahar Hamzei 1
Arezou Khezerlou 2
Ali Ehsani 3
1 School of Agriculture and Natural Resources, Higher Education Institute of Afagh
2 Faculty of Nutrition and Food Science, Tabriz University of Medical Sciences
3 Faculty of Nutrition and Food Science, Tabriz University of Medical Science
چکیده English

Cheese packaging as one of the processes has an important effect on increasing the shelf life and quality of cheese. The aim of this study was to package Iranian white cheese using edible coating based on whey protein isolate (WPI) containing different levels of cardamom (Elettaria cardamomum L.) essential oil (CEO) (0, 1, 1.5 and 2%) during storage. Samples were examined for physicochemical, microbial and sensory changes during 60 days of storage. The chemical compounds of CEO were also identified by GC-MS. In the analysis of the chemical composition of CEO, two main compounds including α-terpenyl acetate (46.705%) and 1.8-cineole (27.415%) were identified. Examination of pH changes showed that the pH of all samples decreased significant during storage (P<0.05). On the last day of storage, the lowest amount of peroxide was in the cheese sample coated with 2% CEO (0.97±0.21 meq O2/kg). The results of changes in TBARS in coated white cheese samples during storage showed that two samples of white cheese coated with WPI enriched with 1.5 and 2% CEO had the lowest Thiobarbituric acid reactive substances levels were from the 7th day to the end of the storage period. In microbiological analysis, the lowest population of total bacteria on the last day of storage was in the white cheese sample enriched with 2% CEO (2.431±0.56 Log cfu/g). The results of sensory evaluation showed that the edible coating based on WPI containing 1.5% of CEO had more desirable sensory properties than other coatings. According to the results of the present study, it can be stated that edible coatings containing plant essential oils can be used in packaging and coating of white cheese to increase its quality and safety.

کلیدواژه‌ها English

White Cheese
packaging
Edible coating
Whey Protein Isolate (WPI)
Cardamom Oil Essential Oil
[1] Khan, I.T., et al., Antioxidant properties of Milk and dairy products: a comprehensive review of the current knowledge. Lipids in health and disease, 2019. 18(1): p. 1-13.
[2] Stanislav, S., et al., Functional dairy products enriched with plant ingredients. Foods and Raw materials, 2019. 7(2): p. 428-438.
[3] Moghaddas Kia, E., M. Alizadeh, and M. Esmaiili, Development and characterization of probiotic UF Feta cheese containing Lactobacillus paracasei microencapsulated by enzyme based gelation method. Journal of food science and technology, 2018. 55(9): p. 3657-3664.
[4] Conte, A., et al., A novel preservation technique applied to fiordilatte cheese. Innovative Food Science & Emerging Technologies, 2013. 19: p. 158-165.
[5] Khezerlou, A. and S.M. Jafari, Nanoencapsulated bioactive components for active food packaging, in Handbook of food nanotechnology. 2020, Elsevier. p. 493-532.
[6] Malmiri, H.J., M.A.G. Jahanian, and A. Berenjian, Potential applications of chitosan nanoparticles as novel support in enzyme immobilization. Am. J. Biochem. Biotechnol, 2012. 8(4): p. 203-219.
[7] Khezerlou, A., et al., Development and characterization of a Persian gum–sodium caseinate biocomposite film accompanied by Zingiber officinale extract. Journal of Applied Polymer Science, 2019. 136(12): p. 47215.
[8] JAFARIZADEH MALMIRI, H., et al., Effects of edible surface coatings (sodium carboxymethyl cellulose, sodium caseinate and glycerol) on storage quality of berangan banana (musa sapientum cv. Berangan) using response surface methodology. Journal of Food Processing and Preservation, 2012. 36(3): p. 252-261.
[9] Jafarizadeh, M.H., et al., Development of an edible coating based on chitosan-glycerol to delay'Berangan'banana (Musa sapientum cv. Berangan) ripening process. International food research journal, 2011. 18(3).
[10] Khezerlou, A., et al., Plant gums as the functional compounds for edible films and coatings in the food industry: A review. Polymers for Advanced Technologies, 2021. 32(6): p. 2306-2326.
[11] Sani, M.A., et al., Recent advances in the development of smart and active biodegradable packaging materials. Nanomaterials, 2021. 11(5): p. 1331.
[12] Yong, H. and J. Liu, Active packaging films and edible coatings based on polyphenol‐rich propolis extract: A review. Comprehensive Reviews in Food Science and Food Safety, 2021. 20(2): p. 2106-2145.
[13] Noumi, E., et al., Chemical and biological evaluation of essential oils from cardamom species. Molecules, 2018. 23(11): p. 2818.
[14] Ahmed, H.M., et al., Phytochemical screening, chemical composition and antimicrobial activity of cinnamon verum bark. International Research Journal of Pure and Applied Chemistry, 2020: p. 36-43.
[15] Sultana, S., F. Ripa, and K. Hamid, Comparative antioxidant activity study of some commonly used spices in Bangladesh. Pakistan journal of biological sciences, 2010. 13(7): p. 340.
[16] Ramos, O.L., et al., Edible films and coatings from whey proteins: a review on formulation, and on mechanical and bioactive properties. Critical reviews in food science and nutrition, 2012. 52(6): p. 533-552.
[17] Nottagh, S., et al., Development of a biodegradable coating formulation based on the biological characteristics of the Iranian Ultra-filtrated cheese. Biologia, 2018. 73(4): p. 403-413.
[18] Torabi, F., H. Jooyandeh, and M. Noshad, Evaluation of physicochemical, rheological, microstructural, and microbial characteristics of synbiotic ultrafiltrated white cheese treated with transglutaminase. Journal of Food Processing and Preservation, 2021. 45(6): p. e15572.
[19] Fox, P.F. and P.L. McSweeney, Advanced dairy chemistry: volume 1: proteins, parts A&B. 2013: Springer.
[20] Jalili, M., Chemical composition and sensory characteristics of Feta cheese fortified with iron and ascorbic acid. Dairy Science & Technology, 2016. 96(4): p. 579-589.
[21] Saravani, M., et al., Gouda cheese spoilage prevention: Biodegradable coating induced by Bunium persicum essential oil and lactoperoxidase system. Food science & nutrition, 2019. 7(3): p. 959-968.
[22] Husain, S.S. and M. Ali, Analysis of volatile oil of the fruits of Elettaria cardamomum (L.) Maton and its antimicrobial activity. World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS), 2014. 3(2): p. 1798-1808.
[23] Mejdi, S., et al., Chemical composition and antimicrobial activities of Elettaria cardamomum L.(Manton) essential oil: a high activity against a wide range of food borne and medically important bacteria and fungi. J. Chem. Biol. Phy. Sci. Sec. A, 2015. 6: p. 248-259.
[24] Mutlu-Ingok, A. and F. Karbancioglu-Guler, Cardamom, cumin, and dill weed essential oils: Chemical compositions, antimicrobial activities, and mechanisms of action against campylobacter spp. Molecules, 2017. 22(7): p. 1191.
[25] Merghni, A., et al., Assessment of the antibiofilm and antiquorum sensing activities of Eucalyptus globulus essential oil and its main component 1, 8-cineole against methicillin-resistant Staphylococcus aureus strains. Microbial pathogenesis, 2018. 118: p. 74-80.
[26] Nottagh, S., et al., Effectiveness of edible coating based on chitosan and Natamycin on biological, physico-chemical and organoleptic attributes of Iranian ultra-filtrated cheese. Biologia, 2020. 75(4): p. 605-611.
[27] Ríos-de-Benito, L.F., et al., Design of an Active Edible Coating Based on Sodium Caseinate, Chitosan and Oregano Essential Oil Reinforced with Silica Particles and Its Application on Panela Cheese. Coatings, 2021. 11(10): p. 1212.
[28] Sayyari, Z. and R. Farahmandfar, Stabilization of sunflower oil with pussy willow (Salix aegyptiaca) extract and essential oil. Food science & nutrition, 2017. 5(2): p. 266-272.
[29] Ahmad, B.S., et al., Fennel oil and by-products seed characterization and their potential applications. Industrial crops and products, 2018. 111: p. 92-98.
[30] Anwar, F., A. Abbas, and K.M. Alkharfy, Cardamom (Elettaria cardamomum Maton) Oils, in Essential oils in food preservation, flavor and safety. 2016, Elsevier. p. 295-301.
[31] Nogueira, M.S., et al., Oxidation products from omega-3 and omega-6 fatty acids during a simulated shelf life of edible oils. LWT, 2019. 101: p. 113-122.
[32] Ksouda, G., et al., Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive. Food chemistry, 2019. 288: p. 47-56.
[33] Vital, A.C.P., et al., Effect of edible and active coating (with rosemary and oregano essential oils) on beef characteristics and consumer acceptability. PloS one, 2016. 11(8): p. e0160535.
[34] Lashkari, H., et al., Effect of Pomegranate Juice on the Manufacturing Process and Characterization of Feta-Type Cheese during Storage. Journal of Food Quality, 2020. 2020.
[35] Mushtaq, M., et al., Use of pomegranate peel extract incorporated zein film with improved properties for prolonged shelf life of fresh Himalayan cheese (Kalari/kradi). Innovative Food Science & Emerging Technologies, 2018. 48: p. 25-32.
[36] El-Sayed, H.S., et al., Development of eco-friendly probiotic edible coatings based on chitosan, alginate and carboxymethyl cellulose for improving the shelf life of UF soft cheese. Journal of Polymers and the Environment, 2021. 29(6): p. 1941-1953.
[37] Motelica, L., et al., Biodegradable antimicrobial food packaging: Trends and perspectives. Foods, 2020. 9(10): p. 1438.
[38] Khalil, M., et al., Production of feta like cheese fortified with pomegranate and lemon peels extract as natural antioxidants. Zagazig Journal of Agricultural Research, 2019. 46(3): p. 710-720.
[39] Rabita, B., et al., Effect of cardamom, thyme and clove powder on the composition and quality of white soft cheese made from goat’s milk. Assiut Journal of Agricultural Science, 2006. 37(4): p. 139-157.
[40] Fekadu, B., et al., Changes in goat milk composition during lactation and their effect on yield and quality of hard and semi-hard cheeses. Small Ruminant Research, 2005. 59(1): p. 55-63.
[41] Park, Y., C. Jeanjulien, and A. Siddique, Factors affecting sensory quality of goat milk cheeses: A Review. J Adv Dairy Res, 2017. 5(185): p. 2.