بررسی فاکتورهای مؤثر بر بازده استخراج ترکیبات فنولی و فلاونوئیدی گیاه به‌لیمو (Aloysia citriodora Palau) تحت امواج فراصوت و بررسی خاصیت آنتی باکتریایی آن

نویسندگان
1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان
2 استادیار گروه مهندسی شیمی، دانشکده مهندسی، دانشگاه زنجان
3 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان
چکیده
یکی از چالش‌های مهم در استفاده از گیاهان دارویی، بهبود بازده استخراج ترکیبات مؤثره گیاهی می‌باشد. بنابراین مطالعه بر روی فاکتورهای اثرگذار در استخراج این مواد از موارد مهمی است که همواره مورد توجه پژوهشگران می‌باشد. گیاه به­لیمو (Aloysia citriodora Palau) به دلیل وجود مواد مؤثره بالا در برگ‌های آن دارای خواص دارویی و غذایی است که همواره مورد توجه پژوهشگران گیاهان دارویی و معطر بوده است. در این پژوهش اثر نوع حلال، دمای استخراج، زمان استخراج و نسبت ماده خشک گیاهی به حلال بر بازده استخراج ترکیبات فنولی، فلاونوئیدی و آنتی اکسیدانی برگ گیاه تحت امواج فراصوت بر مبنای آزمایشات طراحی شده با روش سطح پاسخ مورد بررسی قرار گرفت. نتایج آزمایشات نشان داد که دمای استخراج و نسبت ماده خشک گیاهی به حلال دو متغیر اثرگذار استخراج ترکیبات مؤثره گیه به لیمو تحت امواج فراصوت می‌باشند. همچنین میزان ترکیبات فنولی، فلاونوئیدی و فعالیت آنتی اکسیدانی عصاره آبی- اتانولی در شرایط استخراج مشابه نسبت به آب خالص بیشتر بود. خاصیت ضد باکتریایی عصاره آبی-اتانولی نسبت به عصاره آبی خالص تفاوت معنی داری داشت. حساسیت باکتری اشریشیا کلی نسبت به هر دو عصاره آبی-اتانولی و آبی نسبت به باکتری استافیلوکوکوس آرئوس بیشتر بود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Evaluation of effective factors on the extraction efficiency of phenolic and flavonoid compounds of lemon verbena (Aloysia citriodora Palau) under ultrasonic waves and evaluation of its antibacterial properties

نویسندگان English

Zeinab Samimi 1
Malihehe Yaghoobi 2
Mohsen Sanikhani 3
Azizollah Kheiry 3
1 Department of Horticulture, Faculty of Agriculture, University of Zanjan
2 Assistant Professor, Department of Chemical Engineering, Faculty of Engineering, University of Zanjan
3 Assistant Professor, Department of Horticulture, Faculty of Agriculture, University of Zanjan
چکیده English



One of the major challenges in application of medicinal plants is to improve the extraction efficiency of bioactive materials. Therefore, the study of factors affecting the extraction of these materials is one of the important attractive subject for researchers. Lemon verbena due to the presence of high active substances in its leaves and nutritional properties has always been noticed by researchers of medicinal and aromatic plants. In this study, the effect of solvent type, extraction temperature, extraction time and solid to solvent on the extraction efficiency of phenolic compounds, flavonoid compounds and antioxidant activity of plant leaves under ultrasonic waves was investigated based on designed experiments using response surface methodology (RSM). Our result showed that the extraction temperature and solid to solvent ratio are two effective extraction variables on bioactive compounds extraction of lemon verbena leaves under ultrasonic waves. Also our results showed that aqueous- alcoholic extract has a higher efficiency for extraction of phenolic and flavonoid compounds, and trapping free radicals when compared with pure water. The antibacterial properties of aqueous-alcoholic extract showed a significant difference compared to pure aqueous extract. The susceptibility of Escherichia coli to both aqueous-alcoholic extracts and aqueous extracts was higher than that of Staphylococcus aureus.



کلیدواژه‌ها English

Extraction of Active Substances
Escherichia coli
Antioxidant capacity
Lemon Verbena Extract
response surface methodology
[1]Martínez-Rodríguez, A., Martínez-Olcina, M., Mora, J., Navarro, P., Caturla, N. and Jones, J., 2022. Anxiolytic Effect and Improved Sleep Quality in Individuals Taking Lippia citriodora Extract. Nutrients, 14(1): 1-14.
[2]Sánchez-Marzo, N., Lozano-Sánchez, J., Cádiz-Gurrea, M. D. L. L., Herranz-López, M., Micol, V., and Segura-Carretero, A., 2019. Relationships between chemical structure and antioxidant activity of isolated phytocompounds from lemon verbena. Antioxidants, 8(8), 1-20.
[3]Choupani, M., Arabshahi-Delouee, S. and Alami, M., 2014. Antioxidant properties of various solvent extracts of lemon verbena (Lippia Citriodora) leaves. International Journal of Advanced Biological and Biomedical Research, 2(42): 494-500.
[4]Leyva-Jiménez, F.J., Manca, M.L., Manconi, M., Caddeo, C., Vázquez, J.A., Lozano-Sánchez, J., Escribano-Ferrer, E., Arráez-Román, D. and Segura-Carretero, A., 2020. Incorporation of lippia citriodora microwave extract into total-green biogelatin-phospholipid vesicles to improve its antioxidant activity. Nanomaterials, 10(4): 1-13.
[5]Bondet, V. Brand-Williams, W. and Berset, C., 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. LWT-Food Science and Technology, 30: 609– 615.
[7]Pokorny, J. Yanishlieva, N. and Gordon, M.H., 2001. Antioxidants in Food: Practical Applications. Prague Institute of Chemical Technology, CRC Press, 70- 80.
[6]Noguchi, N. and Niki, E. 2000. Phenolic antioxidants: A rationale for design and evaluation of novel antioxidant drug for atherosclerosis. Free Radical Biology and Medicine, 28: 1538- 1546.
[8]Sarkar, A., and Ghosh, U., 2016. Natural antioxidants-The key to safe and sustainable life. International Journal of Latest Trends in Engineering and Technology, 6(3): 460-466.‌
[9]Caleja, C., Barros, L., Antonio, A. L., Oliveira, M. B. P., & Ferreira, I. C., 2017. A comparative study between natural and synthetic antioxidants: Evaluation of their performance after incorporation into biscuits. Food chemistry, 216: 342-346.‌
[10]Chemat, F., Rombaut, N., Sicaire, A.G., Meullemiestre, A., Fabiano-Tixier, A.S. and Abert-Vian, M., 2017. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics sonochemistry, 34: 540-560.
[11]Allaf, T., Tomao, V., Ruiz, K., and Chemat, F., 2013. Instant controlled pressure drop technology and ultrasound assisted extraction for sequential extraction of essential oil and antioxidants. Ultrasonics Sonochemistry, 20(1): 239-246.‌
[12]Jamalabadi, M., Saremnezhad, S., Bahrami, A., and Jafari, S. M., 2019. The influence of bath and probe sonication on the physicochemical and microstructural properties of wheat starch. Food Science & Nutrition, 7(7): 2427-2435.
[13]Kumar, K., Srivastav, S. and Sharanagat, V.S., 2021. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review. Ultrasonics Sonochemistry, 70: 1-11.
[14] Siregar, A. R., Maeda, Y., Yoshino, T., and Tanaka, T., 2020. The Effects of Solvents and Solid-to-Solvent Ratios on Ultrasound-Assisted Extraction of Carotenoids from Chlorella vulgaris. International Journal of Technology, 11(5): 941-950.‌
[15]Giorgis, M., Garella, D., Cena, C., Boffa, L., Cravotto, G., and Marini, E., 2017. An evaluation of the antioxidant properties of Arthrospira maxima extracts obtained using non-conventional techniques. European Food Research and Technology, 243(2): 227-237.‌
[16] Dzah, C. S., Duan, Y., Zhang, H., Wen, C., Zhang, J., Chen, G., and Ma, H., 2020. The effects of ultrasound assisted extraction on yield, antioxidant, anticancer and antimicrobial activity of polyphenol extracts: A review. Food Bioscience, 35: 100547.‌
[17]Durazzo, A., Lucarini, M., Souto, E. B., Cicala, C., Caiazzo, E., Izzo, A. A., ... and Santini, A., 2019. Polyphenols: A concise overview on the chemistry, occurrence, and human health. Phytotherapy Research, 33(9): 2221-2243.‌
[18]Lohvina, H., Sándor, M., and Wink, M., 2022. Effect of Ethanol Solvents on Total Phenolic Content and Antioxidant Properties of Seed Extracts of Fenugreek (Trigonella foenum-graecum L.) Varieties and Determination of Phenolic Composition by HPLC-ESI-MS. Diversity, 14(1): 1-21.
[19]Yaghoobi, M., Sanikhani, M., Samimi, Z., and Kheiry, A., 2022. Selection of a suitable solvent for bioactive compounds extraction of myrtle (Myrtus communis L.) leaves using ultrasonic waves. Journal of Food Processing and Preservation, e16357.
[20] Mokrani, A., and Madani, K., 2016. Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Separation and Purification Technology, 162: 68-76.‌
[21]Antony, A., and Farid, M., 2022. Effect of temperatures on polyphenols during extraction. Applied Sciences, 12(4): 2107-2114.‌
[22]Santos, J. S., Deolindo, C. T. P., Esmerino, L. A., Genovese, M. I., Fujita, A., Marques, M. B., and Granato, D., 2016. Effects of time and extraction temperature on phenolic composition and functional properties of red rooibos (Aspalathus linearis). Food Research International, 89: 476-487.‌
[23]Benchikh, Y., and Louailèche, H., 2014. Effects of extraction conditions on the recovery of phenolic compounds and in vitro antioxidant activity of carob (Ceratonia siliqua L.) pulp. Acta Botanica Gallica, 161(2): 175-181.‌
[24]Pereira, D. M., Valentão, P., Pereira, J. A., and Andrade, P. B., 2009. Phenolics: From chemistry to biology. Molecules, 14(6): 2202-2211.‌
[25]Falah, S., Suzuki, T., & Katayama, T., 2008. Chemical constituents from Swietenia macrophylla bark and their antioxidant activity. Pakistan Journal of Biological Sciences, 11(16): 2007-2012.‌
[26]Katsarou, A., Rhizopoulou, S., & Kefalas, P., 2012. Antioxidant potential of the aerial tissues of the mistletoe Loranthus europaeus Jacq. Records of Natural Products, 6(4): 394-402.
[27]Mrvčić, J., Posavec, S., Kazazić, S., Stanzer, D., Peša, A., and Stehlik-Tomas, V., 2012. Spirit drinks: a source of dietary polyphenols. Croatian journal of food science and technology, 4(2): 102-111.‌
[28]Nayaka, N. M. D. M. W., Sasadara, M. M. V., Sanjaya, D. A., Yuda, P. E. S. K., Dewi, N. L. K. A. A., Cahyaningsih, E., and Hartati, R., 2021. Piper betle (L): Recent review of antibacterial and antifungal properties, safety profiles, and commercial applications. Molecules, 26(8): 1-21.
[29] Al-Talib, H., Ali, N. D. M., Suhaimi, M. H., Rosli, S. S. N., Othman, N. H., Mansor, N. A. S., and Al-Khateeb, A., 2016. Antimicrobial effect of Malaysian vegetables against enteric bacteria. Asian Pacific journal of tropical biomedicine, 6(3): 211-215.‌
[30] Elisha, I. L., Botha, F. S., McGaw, L. J., and Eloff, J. N., 2017. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complementary and Alternative Medicine, 17(1): 1-10.‌