ارزیابی خصوصیات پروبیوتیکی و ضد قارچی باکتری اسید لاکتیک غالب جدا شده از آرد بلوط (Quercus persica) تخمیر شده

نویسندگان
حسین پورعبدالله 1
علیرضا صادقی 1
مریم ابراهیمی 2
مهدی کاشانی نژاد 1
هدی شهیری طبرستانی 1
جلال محمدزاده 3
1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان
2 مرکز تحقیقات سلامت فراورده‌های غذایی، دارویی و طبیعی، دانشگاه علوم پزشکی گلستان
3 مرکز تحقیقات جهاد کشاورزی
10.52547/fsct.19.124.171
چکیده
ارزیابی خصوصیات باکتری­های اسید لاکتیک جدا شده از بستره­های تخمیری که کمتر مورد مطالعه قرار گرفته­اند همواره احتمال مواجهه با جدایه­های منحصر به فرد را در پی دارد. در این پژوهش، باکتری اسید لاکتیک غالب با تکرار فرایند مایه­گیری از تخمیر آرد بلوط، جداسازی و با استفاده از PCR شناسایی گردید. سپس ویژگی­های پروبیوتیکی (شامل مقاومت به اسید و صفرا، ضد باکتریایی، خود اتصالی و دگر اتصالی، مقاومت آنتی­بیوتیکی و قابلیت همولیز خون) و همچنین اثر ضد قارچی این جدایه لاکتیکی مورد مطالعه قرار گرفت. توالی­یابی محصولات PCR منجر به شناسایی پدیوکوکوس اسیدی­لاکتیس به عنوان جدایه لاکتیکی غالب گردید. جدایه مذکور در تیمار متوالی اسید و صفرا، 72 درصد زنده­مانی خود را حفظ نمود. همچنین تاثیر بازدارنده جدایه لاکتیکی بر روی باسیلوس سرئوس به شکل معنی­داری (05/0P<) از سایر شاخص­های باکتریایی غذازاد بیشتر بود. علاوه بر این، روماند فاقد سلول خام حاصل از کشت پدیوکوکوس اسیدی­لاکتیس جدا شده نیز به طور کامل از رشد باسیلوس سرئوس ممانعت کرد اما روماند فاقد سلول خنثی شده آن تاثیر بازدارنده­ای بر سالمونلا انتریکا نداشت. جدایه لاکتیکی دارای قابلیت خود اتصالی و دگر اتصالی با اشرشیا کلی نیز بود و رفتار همولیزی از خود نشان نداد. اثر ضد قارچی جدایه لاکتیکی در برابر آسپرژیلوس نایجر تایید گردید. با توجه با قابلیت­های پروبیوتیکی و ضد قارچی مناسب پدیوکوکوس اسیدی­لاکتیس جدا شده از تخمیر آرد بلوط می­توان از آن به عنوان کشت میکروبی آغازگر، همراه، پروبیوتیک و یا محافظت کننده در صنایع تخمیری استفاده نمود.
کلیدواژه‌ها
بلوط تخمیر شده
جدایه لاکتیکی غالب
خصوصیات پروبیوتیکی
روماند فاقد سلول

موضوعات

عنوان مقاله English

Evaluation of probiotic and antifungal properties of the predominant LAB isolated from fermented acorn (Quercus persica)

نویسندگان English

Hosein Purabdolah 1
Alireza Sadeghi 1
Maryam Ebrahimi 2
Mahdi Kashaninejad 1
Hoda Shahiri tabarestani 1
Jalal Mohamadzadeh 3
1 Department of Food Science & Technology, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran.
2 Food, Drug and Natural Products Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
3 Agricultural Engineering Research Department ,Golestan Agricultural and Natural Resources Research Center, AREEO, Gorgan, Iran
چکیده English

Characterization of lactic acid bacteria (LAB) isolated from rarely studied fermentation substrates can lead to isolate unique microorganisms. In the present study, predominant LAB was isolated from fermented acorn using repeat of back-slopping process, and then the isolate was identified by PCR. Subsequently, probiotic properties of the isolate (including resistance to acid and bile, antibacterial, auto and co-aggregations, antibiotic susceptibility and blood hemolysis), as well as its antifungal effect were studied. Sequencing results of the PCR products led to the identification of Pediococcus acidilactis as predominant LAB isolate. The survival percentage of the isolate in continuous acid and bile treatment was equal to 72%. The inhibitory effect of the isolate on Bacillus cereus was also significantly (P<0.05) higher than the other foodborne indicator bacteria. Furthermore, crude cell free supernatant (CFS) obtained from LAB culture completely inhibit the growth of B. cereus; meanwhile, its naturalized CFS had no inhibitory effect on Salmonella enterica. LAB isolate had also proper auto and co-aggregation (with E. coli) potentials and had no hemolytic activity. Antifungal activity of the isolate against Aspergillus niger was also verified. By considering the proper probiotic and antifungal potentials of the P. acidilactis isolated from fermented acorn it is possible to use the isolate as microbial starter, adjunct, probiotic and or protective culture in fermentation industries.

کلیدواژه‌ها English

fermented acorn
predominant LAB isolate
probiotic properties
cell free supernatant
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