استخراج و بررسی ویژگی‌های عملکردی پروتئین سبوس برنج و امکان استفاده از آن به‌عنوان جایگزین پروتئین شیر در دسر لبنی کم‌چرب فراسودمند

نویسندگان
عاطفه محمدی 1
سید احمد شهیدی 1
علی رافع 2
شهرام نقی زاده رئیسی 1
آزاده قربانی حسن سرایی 1
1 گروه علوم و صنایع غذایی، دانشگاه آزاد اسلامی واحد آیت ا… آملی
2 گروه فرآوری مواد غذایی، مؤسسه علوم و صنایع غذایی
10.52547/fsct.19.124.157
چکیده
پروتئین سبوس برنج یک پروتئین گیاهی ارزشمند است که به دلیل دارا بودن ویژگی‌های هایپوآلرژنیک، تغذیه‌ای و عملکردی قابل‌قبول، در سال‌های اخیر موردتوجه زیادی قرار گرفته است. در این پژوهش با هدف تولید دسر لبنی کم‌چرب برای مصرف کودکان حساس به پروتئین شیر، پروتئین سبوس برنج با روش قلیایی از سبوس برنج رقم چمپا استخراج و بعد از بررسی ویژگی‌های عملگری شامل حلالیت، ظرفیت و پایداری کف، ظرفیت جذب آب/روغن و ویژگی‌های امولسیفایری، به‌جای پروتئین شیر برای تهیه دسر لبنی استفاده شد. تأثیر این جایگزینی بر ویژگی‌های فیزیکو شیمیایی، حسی و بافت دسر مورد بررسی قرار گرفت. ویژگی‌های عملکردی پروتئین سبوس برنج به‌طور معنی‌داری به pH محیط بستگی داشت (05/0 > P). ظرفیت جذب آب 21/4 گرم بر گرم و جذب روغن 87/3 گرم بر گرم بود. نتایج مربوط به ویژگی‌های عملکردی بیانگر قابلیت استفاده از پروتئین سبوس برنج به‌عنوان جزئی از فرمولاسیون غذاهای فراسودمند ازلحاظ عملکردی می‌باشد. جایگزینی پروتئین سبوس برنج با پروتئین شیر در دسر لبنی کم‌چرب منجر به افزایش شاخص زردی و کاهش شاخص سفیدی دسر شد، pH دسر کم شد ولی آب اندازی تغییر معنی‌دار نکرد. ویژگی بافتی شامل کار کل، سختی، پیوستگی و چسبندگی و همچنین ویژگی‌های حسی دسر لبنی حاوی پروتئین سبوس برنج کاهش معنی‌داری نسبت به نمونه دسر حاوی پروتئین لبنی داشتند (05/0 > P).
کلیدواژه‌ها
دسر لبنی کم‌چرب
پروتئین سبوس برنج
ویژگی‌های عملکردی
بافت

موضوعات

عنوان مقاله English

Extraction and characterization of rice bran protein and its utilization in low-fat dairy dessert as a substitute for dairy protein

نویسندگان English

Atefeh Mohammadi 1
Seyed-Ahmad Shahidi 1
Ali Rafe 2
Shahram Naghizadeh Raeisi 1
Azade Ghorbani-HasanSaraei 1
1 Department of Food Science and Technology, Islamic Azad University, Ayatollah Amoli Branch
2 Department of Food Processing, Research Institute of Food Science and Technology (RIFST)
چکیده English

Rice bran protein is a valuable plant protein that has received much attention in recent years due to its unique functional, nutritional, and hypoallergenic properties. The purpose of this paper is to study the utilization of the rice bran protein (RBP) in low-fat dairy dessert for children who have been shown milk protein allergy. Protein concentrates were prepared from defatted rice bran and analyzed for their functional properties. Rice (Champa Variety) bran proteins were prepared by alkaline extracted. The functional properties of rice bran protein (solubility, foaming capacity and stability, water and oil absorption capacity and Emulsifying properties) were determined. Also physicochemical, sensory properties and texture of low-fat dairy dessert were determined. It was found that pH was significantly effective in the RBP functional properties (P<0.05). Water absorption capacity and oil absorption capacity of RBP were obtained 4.21 g/g and 3.87 g/g, respectively. The results indicated that RBP concentrate can be effectively used for various functional food formulations like dairy dessert. The effect of protein on the color properties of dairy dessert was significant. Whiteness Index was, however, decreased by increasing rice bran protein but yellowness index was increased by increasing rice bran protein in the dessert. There was no significant difference between protein-containing dessert samples (P<0.05). Textural characteristics of dairy dessert containing rice bran protein were significantly different from dairy dessert containing milk protein. Dairy dessert containing rice bran protein showed more desirable sensory properties than the protein-free sample but dairy dessert containing skim milk was better.

کلیدواژه‌ها English

Low fat dairy dessert
Rice bran protein
Functional properties
texture
[1] Vandenplas, Y., De Greef, E. and Devreker, T., 2014. Treatment of cow's milk protein allergy. Pediatric gastroenterology, hepatology & nutrition, 17(1), pp.1-5.
[2] Joeckel, R.J. and Phillips, S.K., 2009. Overview of infant and pediatric formulas. Nutrition in clinical practice, 24(3), pp.356-362.
[3] Salvatore, S. and Vandenplas, Y., 2016. Hydrolyzed proteins in allergy. In Protein in Neonatal and Infant Nutrition: Recent Updates (Vol. 86, pp. 11-27). Karger Publishers.
[4] Martin, C., Lange, C. and Marette, S., 2021. Importance of additional information, as a complement to information coming from packaging, to promote meat substitutes: A case study on a sausage based on vegetable proteins. Food Quality and Preference, 87, p.104058.
[5] Lee, J.S., Oh, H., Choi, I., Yoon, C.S. and Han, J., 2022. Physico-chemical characteristics of rice protein-based novel textured vegetable proteins as meat analogues produced by low-moisture extrusion cooking technology. LWT, p.113056.
[6] Clark, A., Soni, B.K., Sharkey, B., Acree, T., Lavin, E., Bailey, H.M., Stein, H.H., Han, A., Elie, M. and Nadal, M., 2022. Shiitake mycelium fermentation improves digestibility, nutritional value, flavor and functionality of plant proteins. LWT, p.113065.
[7] Cordelle, S., Redl, A. and Schlich, P., 2022. Sensory acceptability of new plant protein meat substitutes. Food Quality and Preference, p.104508.
[8] Chandi, G.K. and Sogi, D.S., 2007. Functional properties of rice bran protein concentrates. Journal of Food Engineering, 79(2), pp.592-597.
[9] Esmaeili, M., Rafe, A., Shahidi, S.A. and Ghorbani Hasan‐Saraei, A., 2016. Functional properties of rice bran protein isolate at different pH levels. Cereal chemistry, 93(1), pp.58-63.
[10] Tayefe, M., Shahidi, S.A., Milani, J.M. and Sadeghi, S.M., 2020. Development, optimization, and critical quality characteristics of new wheat-flour dough formulations fortified with hydrothermally-treated rice bran. Journal of Food Measurement and Characterization, 14(5), pp.2878-2888.
[11] Rafe, A., Vahedi, E. and Hasan‐Sarei, A.G., 2016. Rheology and microstructure of binary mixed gel of rice bran protein–whey: effect of heating rate and whey addition. Journal of the Science of Food and Agriculture, 96(11), pp.3890-3896.
[12] Biglarian, N., Atashzar, A., Rafe, A. and Shahidi, S.A., 2022. Effect of rice bran protein and modified basil seed gum on physicochemical properties and stability of confectionery cream. Journal of food science and technology (Iran), 18(121), pp.13-24.
[13] Wang, M., Hettiarachchy, N.S., Qi, M., Burks, W. and Siebenmorgen, T., 1999. Preparation and functional properties of rice bran protein isolate. Journal of agricultural and food chemistry, 47(2), pp.411-416.
[14] Tang, S., Hettiarachchy, N.S., Horax, R. and Eswaranandam, S., 2003. Physicochemical properties and functionality of rice bran protein hydrolyzate prepared from heat stabilized defatted rice bran with the aid of enzymes. Journal of food science, 68(1), pp.152-157.
[15] Naghizadeh Raeisi, S., Mohamadi Rami, A., Shahidi, S.A. and Ghorbani-HasanSaraei, A., 2019. Functional characteristics of rice bran protein isolate (Hashemi cultivar). Food Science and Technology, 15(85), pp.467-478.
[16] Tárrega, A. and Costell, E., 2006. Effect of inulin addition on rheological and sensory properties of fat-free starch-based dairy desserts. International Dairy Journal, 16(9), pp.1104-1112.
[17] Ares, G., Baixauli, R., Sanz, T., Varela, P. and Salvador, A., 2009. New functional fibre in milk puddings: Effect on sensory properties and consumers' acceptability. LWT-Food Science and Technology, 42(3), pp.710-716.
[18] Ghorbani‐HasanSaraei, A., Rafe, A., Shahidi, S.A. and Atashzar, A., 2019. Microstructure and chemorheological behavior of whipped cream as affected by rice bran protein addition. Food science & nutrition, 7(2), pp.875-881.
[19] El-Garawany, G.A. and Abd El Salam, M.H., 2005. Preparation and rheological properties of a dairy dessert based on whey protein/potato starch. Food chemistry, 91(2), pp.261-267.
[20] Szwajgier, D. and Gustaw, W., 2015. The addition of malt to milk-based desserts: Influence on rheological properties and phenolic acid content. LWT-Food Science and Technology, 62(1), pp.400-407.
[21] Tárrega, A., Durán, L. and Costell, E., 2004. Flow behaviour of semi-solid dairy desserts. Effect of temperature. International dairy journal, 14(4), pp.345-353.
[22] Majzoobi, M., Ghiasi, F. and Farahnaky, A., 2016. Physicochemical assessment of fresh chilled dairy dessert supplemented with wheat germ. International journal of food science & technology, 51(1), pp.78-86.
[23] Verbeken, D., Bael, K., Thas, O. and Dewettinck, K., 2006. Interactions between κ-carrageenan, milk proteins and modified starch in sterilized dairy desserts. International Dairy Journal, 16(5), pp.482-488.
[24] Singh, V., David, J., Kishor, K. and Mishra, S., 2018. Effect of different level of rice flour on physico-chemical properties of low fat frozen dessert. European Journal of Biotechnology and Bioscience, 6(5), pp.01-04.
[25] Depypere, F., Verbeken, D., Torres, J.D. and Dewettinck, K., 2009. Rheological properties of dairy desserts prepared in an indirect UHT pilot plant. Journal of food engineering, 91(1), pp.140-145.
[26] Buriti, F.C. and Saad, S.M., 2014. Chilled milk-based desserts as emerging probiotic and prebiotic products. Critical reviews in food science and nutrition, 54(2), pp.139-150.
[27] McSweeney, P.L. and McNamara, J.P., 2022. Encyclopedia of dairy sciences (Third Edition). Academic Press. pp. 489-497.
[28] Gupta, S., Chandi, G.K. and Sogi, D.S., 2008. Effect of extraction temperature on functional properties of rice bran protein concentrates. International Journal of Food Engineering, 4(2).
[29] AOAC, 2010. Official methods of analysis of AOAC International. Volume I, agricultural chemicals, contaminants, drugs/edited by William Horwitz. Gaithersburg (Maryland): AOAC International, 1997.
[30] Rafe, A., Mousavi, S.S. and Shahidi, S.A., 2014. Dynamic rheological behavior of rice bran protein (RBP): Effects of concentration and temperature. Journal of Cereal Science, 60(3), pp.514-519.
[31] Seuvre, A.M., Turci, C. and Voilley, A., 2008. Effect of the temperature on the release of aroma compounds and on the rheological behaviour of model dairy custard. Food chemistry, 108(4), pp.1176-1182.
[32] Siamand, R., Deeth, H.C. and Al-Saadi, J.M., 2014. Textural and sensory properties of a calcium-induced milk gel. Journal of Food Engineering, 139, pp.10-12.
[33] Patsanguan, S., Hisaranusorn, N., Phongthai, S. and Rawdkuen, S., 2014. Rice bran protein isolates: Preparation and their physico-chemical and functional properties. Food and Applied bioscience journal, 2(3), pp.169-182.
[34] Furlán, L.T.R. and Campderrós, M.E., 2017. The combined effects of Stevia and sucralose as sugar substitute and inulin as fat mimetic on the physicochemical properties of sugar-free reduced-fat dairy dessert. International Journal of Gastronomy and Food Science, 10, pp.16-23.
[35] Rafe, A. and Sadeghian, A., 2017. Stabilization of Tarom and Domesiah cultivars rice bran: Physicochemical, functional and nutritional properties. Journal of Cereal Science, 74, pp.64-71.
[36] Jiamyangyuen, S., Srijesdaruk, V. and Harper, W.J., 2005. Extraction of rice bran protein concentrate and its application in bread. Extraction, 27(1), p.56.
[37] Keshavarz, H.A., Aelami, M., Motamedzadegan, A., Maghsoudloo, Y., Ghorbani, M. And Daraei, G.A., 2012. Chemical composition and physicochemical properties of iranian rice bran. Journal of Innovation in Food Science and Technology, 4(3), Pp.1-8.
[38] Aletor, O.L.U.W.A.T.O.Y.I.N., Oshodi, A.A. and Ipinmoroti, K., 2002. Chemical composition of common leafy vegetables and functional properties of their leaf protein concentrates. Food chemistry, 78(1), pp.63-68.
[39] Foegeding, E.A., 2006. Food biophysics of protein gels: A challenge of nano and macroscopic proportions. Food Biophysics, 1(1), pp.41-50.
[40] Lawal, O.S., 2004. Functionality of African locust bean (Parkia biglobossa) protein isolate: effects of pH, ionic strength and various protein concentrations. Food Chemistry, 86(3), pp.345-355.
[41] Seena, S. and Sridhar, K.R., 2005. Physicochemical, functional and cooking properties of under explored legumes, Canavalia of the southwest coast of India. Food Research International, 38(7), pp.803-814.
[42] El Nasri, N.A. and El Tinay, A.H., 2007. Functional properties of fenugreek (Trigonella foenum graecum) protein concentrate. Food chemistry, 103(2), pp.582-589.
[43] Narsimhan, G. and Xiang, N., 2018. Role of proteins on formation, drainage, and stability of liquid food foams. Annual review of food science and technology, 9, pp.45-63.
[44] Cao, X., Wen, H., Li, C. and Gu, Z., 2009. Differences in functional properties and biochemical characteristics of congenetic rice proteins. Journal of cereal science, 50(2), pp.184-189.
[45] Kaur, M. and Singh, N., 2007. Characterization of protein isolates from different Indian chickpea (Cicer arietinum L.) cultivars. Food Chemistry, 102(1), pp.366-374.
[46] Oladele, A.K. and Aina, J.O., 2007. Chemical composition and functional properties of flour produced from two varieties of tigernut (Cyperus esculentus). African Journal of Biotechnology, 6(21).
[47] McClements, D.J., 2004. Food emulsions: principles, practices, and techniques. CRC press.
[48] Theerakulkait, C., Chaiseri, S. and Mongkolkanchanasiri, S., 2006. Extraction and some functional properties of protein extract from rice bran. Agriculture and Natural Resources, 40(5), pp.209-214.
[49] Gilbert, A., Rioux, L.E., St-Gelais, D. and Turgeon, S.L., 2020. Characterization of syneresis phenomena in stirred acid milk gel using low frequency nuclear magnetic resonance on hydrogen and image analyses. Food Hydrocolloids, 106, p.105907.
[50] Lee, K.Y., Shim, J., Bae, I.Y., Cha, J., Park, C.S. and Lee, H.G., 2003. Characterization of gellan/gelatin mixed solutions and gels. LWT-Food Science and Technology, 36(8), pp.795-802.
[51] Nieuwland, M., Bouwman, W.G., Pouvreau, L., Martin, A.H. and de Jongh, H.H., 2016. Relating water holding of ovalbumin gels to aggregate structure. Food Hydrocolloids, 52, pp.87-94.
[52] Li, Y., Lu, F., Luo, C., Chen, Z., Mao, J., Shoemaker, C. and Zhong, F., 2009. Functional properties of the Maillard reaction products of rice protein with sugar. Food chemistry, 117(1), pp.69-74.
[53] Li, Y., Zhong, F., Ji, W., Yokoyama, W., Shoemaker, C.F., Zhu, S. and Xia, W., 2013. Functional properties of Maillard reaction products of rice protein hydrolysates with mono-, oligo-and polysaccharides. Food Hydrocolloids, 30(1), pp.53-60.
[54] Phantuwong, N., 2017. Functional and Biological Properties of Sang Yod Rice Bran Hydrolysate Prepared by enzymatic Hydrolysis and Its Application in Rice Pudding Product (Doctoral dissertation, Prince of Songkla University).
[55] Kazemi-Taskooh, Z. and Varidi, M., 2021. Designation and characterization of cold-set whey protein-gellan gum hydrogel for iron entrapment. Food Hydrocolloids, 111, p.106205.
[56] Jang, B.K. and Matsubara, H., 2005. Influence of porosity on hardness and Young's modulus of nanoporous EB-PVD TBCs by nanoindentation. Materials Letters, 59(27), pp.3462-3466.