بررسی و مطالعه امکان آلودگی میان بافتی سبزیجات به باکتریهای بیماریزای گوارشی در اثر جذب و انتقال آن از طریق ریشه گیاه

نویسندگان
1 سبزکوشان صنعتی پاژه
2 شرکت سبزکوشان صنعتی پاژه
3 شرکت سبز کوشان صنعتی پاژه
چکیده


سبزیجات تازه منابع مهم مواد مغذی، ویتامین و فیبر بوده و مصرف آنها به ویژه به صورت خام در سالهای اخیر به دلیل توجه ویژه به ارتقای سطح سلامت جامعه افزایش یافته است، امروزه با وجود به کارگیری روشهای گندزدایی سختگیرانه در کارخانجات تولید سبزی و سالاد، همچنان مشکل آلودگیهای میکروبی بخصوص آلودگی به باکتری مزوفیل اشرشیاکلی در فصل تابستان باقی است.

هدف از این تحقیق، بررسی احتمال امکان وجود آلودگی میان بافتی سبزیجات به کلی فرمها و به طور اختصاصی باکتری اشرشیاکلی میباشد.در این تحقیق از نمونههای کاهو آیسبرگ و سبزی گندزدایی نشده به عنوان نمونههای شاهد و ۴ نمونه کاهو و سبزی گندزدایی شده که به طور تصادفی از خط تولید نمونهگیری شد، استفاده گردید. نمونهها پس از طی مراحلگندزدایی با محلول پراستیک اسید و هیپوکلریت کلسیم و سپس آبکشینهایی با آب، به محیط کشت مربوطه تلقیح شد و جهت بررسی آلودگی سطحی، پس از طی زمانهای 1 ، 5 و20 دقیقه و همچنین برای بررسی آلودگی میان بافتی به صورت بسیار ریز خرد شده پس از ۲۰ دقیقه، آزمون اختصاصی جستجوی باکتری اشرشیاکلی انجام شد. رشد این باکتری در نمونههای شاهد و نمونههای سبزی به صورت بسیار ریز خرد شده، مشاهده گردید. نتایج این تحقیق حاکی از صحت و کفایت پروسه گندزدایی و عدم آلودگی سطحی و احتمال وجود آلودگی میان بافتی و جذب این باکتری از طریق ریشه و آوندهای گیاه میباشد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigation and study of the possibility of interstitial contamination of vegetables with gastrointestinal pathogenic bacteria due to its absorption and transfer through plant roots

نویسندگان English

Farahnaz Rahiminejad 1
Amirhossein Khosravi 2
fatemeh mehrazin 3
1 Sabzkooshan Sansti Pazheh
2 Sabzkooshan sanati pazheh Co.
3 Sabzkooshan sanati pazheh Co.
چکیده English

Fresh vegetables are important sources of nutrients, vitamins and fiber and their consumption, especially in raw form, has increased in recent years due to special attention to improving the health of society. Today, despite the use of strict disinfection methods in vegetable and salad factories, The problem of microbial contamination, especially Escherichia coli mesophilic bacteria, remains in the summer. The aim of this study was to investigate the possibility of interstitial contamination of vegetables with coliforms and specifically Escherichia coli. In this study, iceberg lettuce and non-disinfected vegetable samples were used as control samples and 4 samples of disinfected lettuce and vegetables were randomly sampled from the production line. Samples were inoculated into the relevant culture medium after disinfection with peracetic acid solution and calcium hypochlorite and then final rinsing with water. To evaluate the surface contamination, after 1, 5 and 20 minutes and also to examine the interstitial contamination Finely chopped face After 20 minutes, a specific test for Escherichia coli was performed. The growth of this bacterium was observed in control samples and very finely chopped vegetable samples. The results of this study indicate the accuracy and adequacy of the disinfection process and the lack of surface contamination and the possibility of interstitial contamination and absorption of this bacterium through the roots and vessels of the plant.

کلیدواژه‌ها English

Salad and packaged vegetables
Escherichia coli bacteria
Interstitial contamination of vegetables
Contaminated groundwater
Contaminated human and animal fertilizers
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