اثر ضدقارچی اسانس‌ مرزه خوزستانی بر آسپرژیلوس نایجر، بوتریتیس سینه‌را و رایزوپوس استولونیفر عامل پوسیدگی و کپک‌زدگی میوه‌ توت‌فرنگی

نویسندگان
مصطفی رحمتی جنیدآباد 1
بهروز علیزاده بهبهانی 2
محمد نوشاد 2
1 استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.52547/fsct.18.115.13
چکیده
در این مطالعه، اسانس گیاه دارویی مرزه خوزستانی توسط روش تقطیر با آب استخراج شد و محتوای فنول و فلاوونوئید کل، ویژگی آنتی‌اکسیدانی (برپایه روش‌های مهار رادیکال DPPH، رادیکال ABTS و بتا-کاروتن/لینولئیک اسید) و فعالیت ضدقارچی آن در برابر سویه‌های قارچی مولد فساد و پوسیدگی توت‌فرنگی (آسپرژیلوس نایجر، بوتریتیس سینه‏را و رایزوپوس استولونیفر) بررسی گردید. میزان فنول و فلاوونوئید کل اسانس به ترتیب 23/87 میلی‌گرم گالیک اسید بر گرم و 33/69 میلی‌گرم کوئرستین بر گرم گزارش شد. فعالیت آنتی‌اکسیدانی اسانس مطابق روش‌های مهار رادیکال DPPH، مهار رادیکال ABTS و بتا-کاروتن/لینولئیک به ترتیب برابر با 29/63، 30/58 و 19/51 درصد بود. نتایج فعالیت ضدقارچی اسانس (برپایه روش‌های دیسک دیفیوژن آگار، انتشار چاهک و حداقل غلظت مهارکنندگی و کشندگی) نشان داد که بوتریتیس سینه‏را مقاوم‌ترین و آسپرژیلوس نایجر حساس‌ترین سویه‌های قارچی نسبت به اسانس می‏باشند. نتایج حداقل غلظت مهارکنندگی اسانس مرزه خوزستانی برای سویه­های آسپرژیلوس نایجر، بوتریتیس سینه­را و رایزوپوس استولونیفر به ترتیب 50، 100 و 50 میلی­گرم بر میلی­لیتر بود. نتایج حداقل غلظت کشندگی اسانس مرزه خوزستانی برای سویه­های آسپرژیلوس نایجر، بوتریتیس سینه­را و رایزوپوس استولونیفر به ترتیب 200، 400 و 200 میلی­گرم بر میلی­لیتر بود.
کلیدواژه‌ها
اسانس
مرزه خوزستانی
آنتی‌اکسیدان طبیعی
فعالیت ضدقارچی
توت‌فرنگی

موضوعات

عنوان مقاله English

Antifungal effect of Satureja khuzestanica essential oil on Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer causing strawberry’s rot and mold

نویسندگان English

Mostafa Rahmati-Joneidabad 1
Behrooz Alizadeh behbahani 2
Mohammad Noshad 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

In this study, the essential oil of medicinal plant Satureja khuzestanica was extracted using hydrodistillation method and its total phenol and flavonoids contents, antioxidant property (based on DPPH and ABTS radical scavenging and beta-carotene/linoleic acid assays) and antifungal activity against fungi species causing strawberry rot and mold (Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer) were investigated. Based on the results of the total phenol and flavonoid contents of the essential oil were 87.23 mg GAE/g and 69.33 mg QE/g, respectively. The antioxidant activity of the essential oil, based on the DPPH radical scavenging, ABTS radical scavenging, and beta-carotene/linoleic acid assays, were 63.29, 58.30 and 51.19%, respectively. The antifungal activity of the essential oil (based on disk diffusion agar, well diffusion agar, and minimum inhibitory/fungicidal concentration) showed that B. cinerea was the most resistant and A. niger was the most sensitive fungal species to the essential oil. The minimum inhibitory concentration for Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer was 50, 100 and 50 mg/mL, respectively. The minimum fungicidal concentration for Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer was 200, 400 and 200 mg/mL, respectively.

کلیدواژه‌ها English

Essential oil
Satureja khuzestanica
Natural antioxidant
Antifungal activity
Strawberry
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