آلودگی آفلاتوکسینی در پودر سنجد خرده فروشی شده در اردبیل

نویسندگان
استادیار، گروه علوم و صنایع غذایی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
چکیده
مایکوتوکسین ها به عنوان مواد سرطان زا، تراتوژن، ژنوتوکسیک، هپاتوتوکسیک و نفروتوکسیک گزارش شده اند. بنا به مطالعات مروری انجام یافته، انجام مطالعات بیشتر به منظور تعیین آلودگی مایکوتوکسینی در همه انواع مواد غذایی وجود دارد. در مطالعه حاضر، 20 نمونه پودر سنجد به صورت تصادفی-خوشه ای و بر اساس پراکندگی محل های فروش از نواحی مختلف شهر اردبیل تهیه شد. تعیین آلودگی آفلاتوکسینی با استفاده از کروماتوگرافی مایع با کارآیی بالا و خالص سازی با ستون ایمونوافینیتی صورت گرفت. بنا به نتایج، هفده نمونه از کل نمونه ها (85 درصد) آلودگی آفلاتوکسینی با دامنه 0/2360-40/30 میکروگرم در کیلوگرم داشتند. آفلاتوکسین های B1 و B2 در 85 درصد نمونه ها مشاهده شد. آلودگی آفلاتوکسین های G1 و G2 به ترتیب در 80 و 70 درصد نمونه ها گزارش شد. سطح آلودگی افزون بر حداکثر مجاز ایران و اتحادیه اروپا بود. این یافته ها نیاز به پایش بیشتر آلودگی آفلاتوکسینی پودر سنجد و جمعیت مصرف کننده آن را در ایران نشان می دهد.

موضوعات


عنوان مقاله English

Aflatoxin Contamination in Retailed Elaeagnus angustifolia Powder in Ardabil

نویسندگان English

Fatemeh Ghannadiasl
Bahram Fathi achachelooee
Assistant Professor, Department of Food Science and Technology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده English

Mycotoxins have been reported to be carcinogenic, teratogenic, genotoxic, hepato- and nephrotoxic. Based on the literature review, there is a need for more studies on the mycotoxin contamination in all types of foods. In this study, 20 samples of Elaeagnus angustifolia L. powder were purchased randomly and clustered based on the distribution of sales areas from different districts in Ardabil city. The determination of aflatoxin contamination was carried out using high performance liquid chromatography and immunoaffinity column clean-up.

According to the results, seventeen of 20 samples (85%) were found contaminated with aflatoxins in the range of 30.40-2360.0 µg/kg. Both AFB1 and AFB2 were detected in 85% of samples. 80% and 70% samples had AFG1 and AFG2, respectively. The contamination levels were exceeded the maximum limit of Iran and European Union. These findings suggest an urgent need to monitor contamination of aflatoxin and consuming population of Elaeagnus angustifolia L. powder in Iran.

کلیدواژه‌ها English

Aflatoxin
High performance liquid chromatography
Immunoaffinity column clean-up
Elaeagnus angustifolia L. powder
[1] Hamid, A. S., Tesfamariam, I. G., Zhang, Y., & Zhang, Z. G. (2013). Aflatoxin B1-induced hepatocellular carcinoma in developing countries: Geographical distribution, mechanism of action and prevention. Oncology letters, 5(4), 1087-1092.
[2] Katz, G. L., & Shafroth, P. B. (2003). Biology, ecology and management of Elaeagnus angustifolia L. (Russian olive) in western North America. Wetlands, 23(4), 763-777.
[3] Rafie, H., Soheila, H., & Parvin, D. (2019). Chemistry, Pharmacology and Medicinal Property of Russian olive (Elaeagnus angustifolia L). Journal of Cancer Science and Research: Open Access, 6(1), 1-7.
[4] Okmen, G., & Turkcan, O. (2014). A study on antimicrobial, antioxidant and antimutagenic activities of Elaeagnus angustifolia L. leaves. African Journal of Traditional, Complementary and Alternative Medicines, 11(1), 116-120.
[5] Wang, Y., Guo, T., Li, J. Y., Zhou, S. Z., Zhao, P., & Fan, M. T. (2013). Four flavonoid glycosides from the pulps of Elaeagnus angustifolia and their antioxidant activities. In Advanced Materials Research (Vol. 756, pp. 16-20). Trans Tech Publications.
[6] Li, L. H., Baek, I. K., Kim, J. H., Kang, K. H., Koh, Y. S., Jung, Y. D., & Shin, B. A. (2009). Methanol extract of Elaeagnus glabra, a Korean medicinal plant, inhibits HT1080 tumor cell invasion. Oncology Reports, 21(2), 559-563.
[7] Saboonchian, F., Jamei, R., & Sarghein, S. H. (2014). Phenolic and flavonoid content of Elaeagnus angustifolia L. (leaf and flower). Avicenna Journal of Phytomedicine, 4(4), 231.
[8] Natanzi, M. M., Pasalar, P., Kamalinejad, M., Dehpour, A. R., Tavangar, S. M., Sharifi, R., Ghanadian, N., Rahimi-Balaei, M. & Gerayesh-Nejad, S. (2012). Effect of aqueous extract of Elaeagnus angustifolia fruit on experimental cutaneous wound healing in rats. Acta Medica Iranica, 50 (9), 589-596.
[9] Mehrabani, N. M., Nejad, S. G., Kamalinejad, M., Dehpour, A. R., Tavangar, S. M., Sharify, R., Ghannadian, N & Pasalar, P. (2011). Histological changes and wound healing response following use of aqueous extract of Elaeagnus angustifolia in albino rats. Clinical Biochemistry, 13(44), S39.
[10] Ebrahimzadeh, A., Mohammadzadeh Rostami, F., & Salimi, A. (2014). Prevalence of fungal contamination of flours in Zahedan Bakeries in 2013. Medical Journal of Mashhad University of Medical Sciences, 57 (5), 705-710.
[11] Sarma, U. P., Bhetaria, P. J., Devi, P., & Varma, A. (2017). Aflatoxins: Implications on health. Indian Journal of Clinical Biochemistry, 32(2), 124-133.
[12] Reddy, K. R. N., Salleh, B., Saad, B., Abbas, H. K., Abel, C. A., & Shier, W. T. (2010). An overview of mycotoxin contamination in foods and its implications for human health. Toxin Reviews, 29(1), 3-26.
[13] Ostry, V., Malir, F., Toman, J., & Grosse, Y. (2017). Mycotoxins as human carcinogens—the IARC Monographs classification. Mycotoxin Research, 33(1), 65-73.
[14] Williams, J. H., Phillips, T. D., Jolly, P. E., Stiles, J. K., Jolly, C. M., & Aggarwal, D. (2004). Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. The American Journal of Clinical Nutrition, 80(5), 1106-1122.
[15] Cansev, A., Sahan, Y., Celik, G., Taskesen, S., & Ozbey, H. (2011). Chemical properties and antioxidant capacity of Elaeagnus angustifolia L. fruits. Asian Journal of Chemistry, 23(6), 2661-2665.
[16] Baydar, T., Engin, A. B., Girgin, G., Aydin, S., & Sahin, G. (2005). Aflatoxin and ochratoxin in various types of commonly consumed retail ground samples in Ankara, Turkey. Annals of Agricultural & Environmental Medicine, 12(2), 193-197.
[17] Institute of Standards and Industrial Research of Iran, Cereals and cereal products -Sampling. ISIRI No 13535. 1st Edition, Karaj: ISIRI; 2010 [in Persian].
[18] Institute of Standards and Industrial Research of Iran, Food and feed stuffs - Determination of aflatoxins B&G by HPLC method using immunoaffinity column clean up-Test method. ISIRI No 6872. 1st Revision, Karaj: ISIRI; 2011 [in Persian].
[19] Trucksess, M. W., Weaver, C. M., Oles, C. J., Fry, F. S., Noonan, G. O., Betz, J. M., & Rader, J. I. (2008). Determination of aflatoxins B1, B2, G1, and G2 and ochratoxin A in ginseng and ginger by multitoxin immunoaffinity column cleanup and liquid chromatographic quantitation: collaborative study. Journal of AOAC International, 91(3), 511-523.
[20] Institute of Standards and Industrial Research of Iran. Food and Feed. Mycotoxins-Maximum tolerated level. ISIRI No 5925. 1st Edition, Karaj: ISIRI; 2002 [in Persian].
[21] European Commission (EC). Commission Regulation, 165/2010 of February 26th setting maximum levels for certain contaminants in foodstuffs as regards aflatoxins. Officials Journal European :union:. 2010: L50/8. Available at: https://www.fsai.ie/uploadedFiles/Reg165_2010.pdf
[22] Nazari, F., Sulyok, M., Yazdanpanah, H., Kobarfard, F., & Krska, R. (2014). A survey of mycotoxins in domestic rice in Iran by liquid chromatography tandem mass spectrometry. Toxicology Mechanisms & Methods, 24(1), 37-41.
[23] Hussain, Z., Khan, M. Z., Khan, A., Javed, I., Saleemi, M. K., Mahmood, S., & Asi, M. R. (2010). Residues of aflatoxin B1 in broiler meat: Effect of age and dietary aflatoxin B1 levels. Food and Chemical Toxicology, 48(12), 3304-3307.
[24] Marchese, S., Polo, A., Ariano, A., Velotto, S., Costantini, S., & Severino, L. (2018). Aflatoxin B1 and M1: Biological properties and their involvement in cancer development. Toxins, 10(6), 214.
[25] International Agency for Research on Cancer. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. http://monographs. iarc. fr/ENG/Monographs/vol82/volume82. pdf. 2002.
[26] Williams, J. H., Phillips, T. D., Jolly, P. E., Stiles, J. K., Jolly, C. M., & Aggarwal, D. (2004). Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. The American Journal of Clinical Nutrition, 80(5), 1106-1122.
[27] Williams, J. H. (2011). Aflatoxin as a public health factor in developing countries and its influence on HIV and other diseases. Peanut Collaborative Research Support Program, University of Georgia, World Bank Report, 1-95.
[28] Wild, C. P., & Gong, Y. Y. (2009). Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis, 31(1), 71-82.
[29] Kumar, P., Mahato, D. K., Kamle, M., Mohanta, T. K., & Kang, S. G. (2017). Aflatoxins: a global concern for food safety, human health and their management. Frontiers in Microbiology, 7, 2170.
[30] Mahtabani, A., Bayat, M., Hosseini, S.E., Aminafshar, M., & Tavakoli, H. (2011). Assessment of Ochratoxin A and Aflatoxin B1, B2, G1, G2 Rates in Breakfast Grains of Supermarkets in Tehran Using HPLC Method in 2010. Hakim Research Journal, 14(1), 10-15.
[31] Afzali, N., Omidi, A., & Shibak, A. (2013). Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH. Armaghane Danesh Bimonthly Journal, 18(3), 210-8[in Persian].
[32] Zaki, M. M., El-Midany, S. A., Shaheen, H. M., & Rizzi, L. (2012). Mycotoxins in animals: Occurrence, effects, prevention and management. Journal of Toxicology and Environmental Health Sciences, 4(1), 13-28.
[33] Skendi, A., Biliaderis, C. G., Papageorgiou, M., & Izydorczyk, M. S. (2010). Effects of two barley β-glucan isolates on wheat flour dough and bread properties. Food Chemistry, 119(3), 1159-1167.
[34] Huang, C (2007). Mechanism of intraspecific toxin inhibition in Aspergillus flavus. A Thesis for the degree of Master of Science in The Department of Plant Pathology and Crop Physiology, Zhejiang University, China.