بررسی حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی اسانس اکالیپتوس گلوبولوس بر تعدادی از باکتری‌های بیماری‌زا و عامل فساد مواد غذایی

نویسندگان
بهروز علیزاده بهبهانی 1
محمد نوشاد 1
بهاره صحرائیان 2
1 استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
چکیده
گیاهان دارویی از چندین هزار سال پیش به دلیل خاصیت درمانی مورد استفاده قرار گرفته­اند. اکالیپتوس یکی از این گیاهان است که فعالیت ضدمیکروبی آن در بسیاری از مناطق جهان برای درمان سرماخوردگی و آنفولانزا مورد استفاده قرار می­گیرد. هدف از این پژوهش تجربی، ارزیابی فعالیت ضدباکتری اسانس اکالیپتوس گلوبولوس به 4 روش دیسک دیفیوژن، چاهک آگار، میکرودایلوشن براث و تعیین حداقل غلظت کشندگی بر تعدادی از سویه­های بیماری­زا و عامل فساد مواد غذایی در شرایط برون­تنی بود. به طور کلی نمای حساسیتی میکروب­های بیماری­زا در برابر اسانس اکالیپتوس گلوبولوس (دیسک دیفیوژن) از مقاوم­ترین به حساس­ترین سویه به ترتیب شامل سالمونلا تیفی > اشرشیا کلی > سودوموناس ائروژینوزا > باسیلوس سوبتلیس > استرپتوکوکوس پیوژنز > استافیلوکوکوس اورئوس بود. میانگین قطر هاله عدم رشد به روش چاهک آگار برای باکتری­های گرم مثبت 60/18 میلی­متر بود. میانگین قطر هاله عدم رشد برای باکتری­های گرم منفی93/11 میلی­متر بود. نتایج نشان داد که حداقل غلظت مهارکنندگی اسانس اکالیپتوس گلوبولوس برای باکتری­های سالمونلا تیفی، سودوموناس ائروژینوزا، اشرشیا کلی، باسیلوس سوبتلیس، استرپتوکوکوس پیوژنز و استافیلوکوکوس اورئوس به ترتیب 128، 64، 64، 32، 16 و 8 میلی­گرم بر میلی­لیتر بود. نتایج حداقل غلظت کشندگی اسانس اکالیپتوس گلوبولوس نیز به ترتیب 512، 256، 128، 128، 32 و 16 میلی­گرم بر میلی­لیتر بود. پیشنهاد می­شود آزمون­های تکمیلی جهت استفاده از اسانس اکالیپتوس گلوبولوس جهت استفاده به عنوان نگهدارنده طبیعی در صنعت غذا و مصارف دارویی انجام گیرد.
کلیدواژه‌ها
اکالیپتوس گلوبولوس
مقاومت دارویی
نگهدارنده غذایی
فعالیت ضدمیکروبی

موضوعات

عنوان مقاله English

Investigation of the minimum inhibitory concentration and the minimum bactericidal concentration of Eucalyptus globulus essential oil on a number of pathogenic bacteria and the cause of food spoilage

نویسندگان English

Behrooz Alizadeh behbahani 1
Mohammad Noshad 1
Bahareh Sahraiyan 2
1 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 PhD, Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده English

Medicinal plants have been used for its medicinal attributes from several thousand years ago. Eucalyptus is one of these plants which the antimicrobial effects has long been used to treat a cold and influenza in most parts of the world. The aim of this experimental study was to evaluate the antibacterial activity of Eucalyptus globulus essential oil (EGEO) in 4 ways: disc diffusion agar, well diffusion agar, microdilution broth and minimum bactericidal concentration on a number of strains pathogens and the cause of food spoilage in vitro. The sensitivity profile of microorganisms against the EGEO (disc diffusion agar) is as follows form the most resistant to the most sensitive: Salmonella typhi > Escherichia coli > Pseudomonas aeruginosa > Bacillus subtilis > Streptococcus pyogenes > Staphylococcus aureus. The mean inhibition zone in well diffusion agar was equal to 18.62 mm against Gram-positive bacteria. The mean inhibition zone in well diffusion agar was equal to 11.93 mm against Gram-negative bacteria. The results showed that the minimum inhibitory concentration of EGEO for Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Streptococcus pyogenes and Staphylococcus aureus was 128, 64, 64, 32, 16 and 8 mg/ml respectively. The minimum bactericidal concentration for microorganisms was 512, 256, 128, 128, 32 and 16 mg/ml respectively. It is recommended that additional tests be performed to use EGEO for use as a natural preservative in the food and pharmaceutical industry.

کلیدواژه‌ها English

Eucalyptus globulus
Drug resistance
Food preservative
Antimicrobial effect
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