بررسی فعالیت آنتی‌اکسیدانی عصاره بره موم و اثر آن در جلوگیری از اکسیداسیون روغن آفتابگردان

نویسندگان
مازیار اسفندیاری فرد 1
سیدحمیدرضا ضیاءالحق 2
1 دانش آموخته کارشناسی ارشد. گروه علوم و صنایع غذایی، دانشگاه آزاد اسلامی، واحد دامغان، دامغان، ایران.
2 بخش تحقیقات فنی و مهندسی کشاورزی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان سمنان (شاهرود)، سازمان تحقیقات، آموزش و ترویج کشاورزی، شاهرود، ایران
10.52547/fsct.17.107.119
چکیده
بره­موم یک ماده صمغی جمع آوری شده توسط زنبور عسل از منابع گیاهی مختلف است. هدف از این تحقیق، ارزیابی فعالیت آنتی­اکسیدانی عصاره بره­موم در روغن آفتابگردان طی زمان نگهداری بود. در این پژوهش، ابتدا ظرفیت آنتی­اکسیدانی عصاره­های اتانولی، متانولی و آبی بره­موم با استفاده از روش DPPH ارزیابی شد. نتایج نشان داد که عصاره متانولی بره­موم بالاترین فعالیت آنتی­اکسیدانی را داشت. سپس غلظت­های مختلف عصاره متانولی (٠، ٥/٠ و ١ درصد) به روغن آفتابگردان اضافه شده و به مدت ٦٠ روز در دمای ٢٥ درجه سانتی­گراد نگهداری شدند. برای مقایسه عصاره بره موم با آنتی­اکسیدان­های صنعتی، روغن آفتابگردان بازاری حاوی TBHQ نیز تهیه شده و همراه نمونه­ها نگهداری شد. در طی زمان نگهداری، هر ٢٠ روز یک بار، اندیس­های پراکسید (PV)، اسید تیوباربیتوریک (TBARS) و اسیدی اندازه­گیری شدند. داده­های حاصله با استفاده از طرح کاملا تصادفی تجزیه و تحلیل شدند. نتایج نشان داد که افزودن آنتی­اکسیدان به روغن آفتابگردان منجر به کاهش اندیس­های PV، TBARS و اندیس اسیدی نمونه­ها شد. از این نظر، غلظت ١ درصد عصاره متانولی بره موم کارآمدتر از غلظت ٥/٠ درصد و آنتی­اکسیدان سنتزی بود. در طی زمان نگهداری، مقادیر اندیس­های اکسیداتیو نمونه­ها به تدریج افزایش یافت. در همه زمان­های مورد مطالعه، بالاترین میزان اکسیداسیون مربوط به روغن خام بود. نتایج ارزیابی حسی نشان داد که اختلاف معنی­داری بین امتیازات طعم، رنگ و پذیرش کلی روغن حاوی عصاره بره­موم و روغن آفتابگردانِ بازاری وجود نداشت، اما امتیاز مربوط به بو روغن حاوی عصاره بره­موم کمتر از روغن بازاری بود. این گونه به نظر می­رسد که عصاره متانولی بره­موم دارای فعالیت آنتی­اکسیدانی است که می­توان از آن به عنوان یک آنتی­اکسیدان در روغن و محصولات بر پایه روغن طی زمان نگهداری استفاده نمود.
کلیدواژه‌ها
اکسیداسیون روغن
بره موم
خاصیت آنتی‌اکسیدانی
روغن آفتابگردان
فراورده‌های زنبورعسل

موضوعات

عنوان مقاله English

Study on the antioxidant activity of propolis extract and its effect on the oxidation of sunflower oil

نویسندگان English

Mazyar Esfandiarifard 1
seyedhamidreza ziaolhagh 2
1 Graduated Master, Department of Food Science and Technology, Islamic Azad University, Damghan Branch, Damghan, Iran.
2 Agricultural Engineering Research Department, Semnan (Shahrood) Agricultural and Natural Resources Research and Education Center , AREEO, Shahrood, Iran.
چکیده English

Propolis is a resinous substance collected by honeybees from various plant sources. This study was aimed to evaluate antioxidative activities of propolis extract in sunflower oil during the storage period. In this study, the antioxidant capacities of the propolis extracts (ethanol, methanol and aqueous extracts) were determined initially using DPPH and the best extract was selected to add to oil. The results stated that the methanolic extract of propolis had the highest antioxidant activity. Then, different concentrations of methanolic extract (0, 0.5 and 1 percentage) were added to sunflower oil and incubated for 60 days at 25°C. To compare the propolis extract with synthetic antioxidants, commercial sunflower oil containing TBHQ was prepared and incubated, too. Peroxide values (PV), acidity index and thiobarbituric acid-reactive substances (TBARS) levels were measured every 20 days during the period of the study. The abtained data were analysed using completely randomized design. Results showed that the addition of antioxidant to sunflower oil lead to decrease of PV, TBARS and acidity values of samples. In this respect, 1% methanolic extract of propolis was more potent than the 0.5% extracts and synthetic Antioxidant. During storage, the amounts of oxidative indexes of samples were increased gradually. The highest amount of oxidation was observed for crude oil. The sensory results showed no significant different between flavor, color, and overall acceptance scores of oil with propolis extract and commercial Sunflower oil, but the score of odor of oil with propolis extract was lower than the commercial oil. It seems that methanolic extract of propolis is a potent antioxidant, which makes it a potential antioxidant for oil and oily products during storage.


کلیدواژه‌ها English

Antioxidant activity
Oxidation of oil
Propolis
Sunflower oil
bee products
[1] Fatemi, H. 2002. Food chemistry. Enteshar publication.
[2] Haumann, B.F. 1994. Antioxidants: Health implication still debated. Inform, 5:242-252.
[3] Banskota, A. H., Tezuka, Y., Adnyana, I. K., Midorikawa, K., Matsushige, K., Message, D., Huertas, A. A. G., Kadota, S. 2002. Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and China. Journal of Ethnopharmacology, 72(1,2): 239–246.
[4] Burdock, G. A. 1998. Review of the biological properties and toxicity of bee propolis. Food and Chemical Toxicology, 36(4): 347–363.
[5] Grange, J.M., Davey, R.W. 1990. Antibacterial properties of propolis (bee glue). Journal of the Royal Society of Medicine, 83(3): 159–160.
[6] Pourazadi, L., Nehzati, G., Ghaziani, F. and Abbasai, S. 2017. Evaluation the quality and quantity of phenolic compound and antioxidant activity of propolis in the vicinity of Karaj. Iranian Journal of Animal Science. 47(4): 499-506.
[7] Popova, M., Trusheva, B. and Bankova, V., 2017. Content of biologically active compounds in Bulgarian propolis: a basis for its standardization. Bulgarian Chemical Communications. 49:115-120.
[8] Galeotti, F., Maccari, F., Fachini, A. and Volpi, N. 2018. Chemical Composition and Antioxidant Activity of Propolis Prepared in Different Forms and in Different Solvents Useful for Finished Products. Foods. 7(3):41-50.
[9] Pascual, C., Gonzalez, R. and Torricella, R.G. 1994. Scavenging action of propolis extract against oxygen radicals. Journal of Ethnopharmacology, 41(1-2), pp.9-13.
[10] Grange, J.M., Davey, R.W. 1990. Antibacterial properties of propolis (bee glue). Journal of the Royal Society of Medicine, 83(3): 159–160.
[11] Sheikhi Koohsar, A. A., Sayyed-Alangi, S. Z., Shamloofar, M. and Sharifian, S. 2018. Effect of different extracts of Iranian propolis on shelf-life of silver crap (Hypophthalmichthys molitrix) fillet in the refrigerator conditions: Chemical composition, evaluation of microbiological and sensory parameters. Journal of Food Science and Technology. 15(76): 51-65.
[12] Isla, M. I., Nieva, M. I., Sampietro, A. R., Vattuone, M. A. 2001. Antioxidant activity of Argentine propolis extracts. Journal of Ethnopharmacology, 76(2): 165–170.
[13] Amoros, M., Lurton, E., Boustie, J., Girre, L., Sauvager, F., Cormier, M. 1994. Comparison of the anti-herpes simplex virus activities of propolis and 3-methylbut-2-enyl caffeate. Journal of Natural Products, 57(5): 235–240.
[14] Amoros, M., Simoes, C. M. O., Girre, L., Sauvager, F., Cormier, M. 1992. Synergistic effect of flavones and flavonols against Herpes simplex virus type I in cell culture. Comparison with the antiviral activity of propolis. Journal of Natural Products, 55(12): 1732–1740.
[15] Strehl, E., Volpert, R., Elstner, E. F. 1994. Biochemical activities of propolis extracts. III. Inhibition of dihydrofolate reductase. Zeitschrift Fur Naturforschung C-A Journal of Biosciences, 49(1,2): 39–43.
[16] Frankel, E.N. 1985. Chemistry of autoxidation: mechanism, products and flavor significance. In: Min DB, Smouse TH, editors. Flavor chemistry of fats and oils. Champaign, Ill.:American Oil Chemists’ Society. p 1–34.
[17] Marcucci, M. C. 1995. Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26(2): 83–99.
[18] Dobrowolski, J. W., Vohora, S. B., Sharma, K., Shah, A. H., Naqvi, S. A. H., Dandiya, P. C. 1991. Antibacterial, antifungal, antiamoebic, anti-inflammatory and antipyretic studies on propolis bee products. Journal of Ethnopharmacology, 35(1): 77–82.
[19] Ghisalberti, E. 1979. Propolis: a review. Bee World, 60: 59–84.
[20] Bonvehi, J. S., Coll, F. V. 1994. Phenolic composition of propolis from China and from South America. Zeitschrift Fur Naturforschung C-A Journal of Biosciences, 49(11,12): 712–718.
[21] Iran National Organization of Standardization.2018. Animal and vegetable fats and oils -Determination of peroxide value -Iodometric (visual) endpoint determination. 4179.second revision.
[22] Iran National Organization of Standardization. 2011. Animal and vegetable fats and oils -Determination of acid value and acidity- Test method. 4178.
[23] AOCS, A. 1998. Official methods and recommended practices of the American Oil Chemists' Society Method Cd 19-90. 2-Thiobarbituric acid value, fifth ed. American Oil Chemists-Society, Champaign.
[24] Jafari, N., Ziaolhagh, S. H. R. and, MohammadiNafchi, A. 2019. Study on the effect of microwave pre-treatment on the quality of air-dried potato sticks using response surface methodology. Journal of Food Science and Technology. 16(86): 189-198.
[25] Brand-Williams, W., Cuvelier, M.E., Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss U Technology, 28: 25-30.
[26] Mamashloo, S., SADEGHI, M.A., Ghorbani, M., Alami, M. and Khomeiri, M. 2012. The evaluation of antioxidant properties and stability of phenolic copmpounds from medlar (Mespilus germanica L.) fruit. Journal of research and innovation in food science and technology. 1(3):219-228.
[27] Mohagheghi, S.A., Poorazarang, H., Elhamirad, A.H., Dezashibi, Z. and Hematyar, N., 2011. Extraction of phenolic compounds from potato peel (Ramus variety) with solvent and ultrasound-assisted methods and evaluation of its antioxidant activity in soybean oil. Food Science and Technology. 8 (28):81-91.
[28] Malek, F. 2005. Frying oils and fats and frying technology. Tehran. Marze Danesh press. pp. 244-245.
[29] Mousavi, S. 2006. Investigation of oxidation and characteristics of sesame oil. Master's Degree in Agricultural Engineering, Food Science and Technology, Islamic Azad University, Tehran Science Research Branch.
[30] Kalapathy, U. and Proctor, A. 2000. A new method for free fatty acid reduction in frying oil using silicate films produced from rice hull ash. Journal of the American Oil Chemists' Society, 77: 593–598.
[31] Iran National Organization of Standardization. 2016. Edible Fats& Oils-Frying oil – Specifications and Test Methods. Second revision. 4152.
[32] Kaviani, M., Niazmand, R. and Shahidi, N.M. 2013. Discarding time evaluation of canola oil based on oxidation indexes during potato deep frying. Journal of research and innovation in food science and technology. 2(1):37-50.
[33] Matthaus, B. 2006. Utilization of high oleic rapeseed oil for deep fat frying of French fries compared to other commonly used edible oils. European Journal of Lipid Science and Technology, 108(3): 200-211.
[34] Iran National Organization of Standardization.2014. Animal and Vegetable Oils and Fats –Crude.Sunflower Oil-Specifications and Test methods. 10086.
[35] Rafiei, Z., Jafari, S.M., Alami, M. and Khomeiri, M. 2011. Antioxidant properties of olive leaf extract and its application in sunflower oil. Journal of food research (university of tabriz). 21(1):11-23.
[36] Shahidi, I. and Bhanger, M. 2007. Stabilization of sunflower oil by garlic extract during accelerated storage. Food Chemistry, 100: 246-254.
[37] Samad Louei.H., Azizi, M.H. and Barzegar, M. 2007. Antioxidative effect of pomegranate seed phenolic componends on soybean oil. Journal of Agricultural Sciences and Natural Resources. 14(4): 193-200.
[38] Farag, R.S., Mahmoud, E.A. and Basuny, A.M. 2007. Use crude olive leaf juice as a natural antioxidant for the stability of sunflower oil during heating. International Journal of Food Science and Technology, 42: 107-115.
[39] Singh, G., Maurya, S. and Delampasona, M.P. 2007. A comparison of chemical antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food and Chemical Toxicology, 45: 1650-1661.
[40] Alizadeh, L., Nayebzadeh, K and Shahin,R. 2014. Antioxidant effect of rosemary and ferulago extracts and synthetic TBHQ on oil oxidation during deep-frying. Iranian Journal of Nutrition Sciences & Food Technology. 8(4):135-143.