شناسایی ترکیبات شیمیایی و ارزیابی ویژگی های آنتی اکسیدانی و ضدباکتریایی شیره خرمالو وحشی(خرمندی)

نویسندگان
مائده کلبادی نژاد
لیلا نجفیان
گروه علوم و صنایع غذایی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
10.52547/fsct.17.100.151
چکیده
خرمالو وحشی (خرمندی) با نام علمی Diospyros lotus دارای ویژگی­های سلامت بخش فراوانی است که این ویژگی­ها عمدتا به ترکیبات زیست فعال موجود در آن نسبت داده می شود. در مطالعه حاضر، ویژگی­های آنتی اکسیدانی و ضد باکتریایی شیره خرمالو وحشی مورد بررسی قرار گرفت و شناسایی ترکیبات عصاره به کمک دستگاه کروماتوگرافی گازی توام با طیف سنجی جرمی (GC-MS) صورت پذیرفت. محتوای فنل تام و فلاونوئیدی و فعالیت آنتی اکسیدانی عصاره ها اندازه‌گیری شد. فعالیت ضد میکروبی بر روی چهار باکتری به روش دیسک دیفیوژن انجام شد. نتایج حاصل از تحقیق نشان دهنده محتوای بالای فنل تام و ترکیبات فلاوونوئیدی در شیره خرمالوی وحشی بوده است. بررسی خواص آنتی اکسیدانی شیره خرمالوی وحشی نیز نشان دهنده ویژگی بالای آنتی اکسیدانی آن بوده است. میزان مهار تشکیل رادیکال DPPH توسط شیره خرمالوی وحشی در غلظتهای 800، 400، 200، 100 و 50 میلی گرم/میلی لیتر از DPPH به ترتیب برابر با 0/82%، 53/61%، 51/53%، 52/32% و 31/18% بوده است. خواص ضدمیکروبی شیره خرمالوی وحشی به ویژه در رقت های بالا نظیر 50 و 100اثرات مهارکننده گی بالایی بر روی رشد سویه های مختلف از جمله اشریشیاکلی، سالمونلا تیفی موریوم، استافیلوکوکوس ارئوس و باسیلوس سرئوس داشت. نتایج حاصل از شناسایی ترکیبات شیمیایی نشان داد که شیره خرمالو وحشی حاوی مقادیر بالایی از قند و اجزای عملکردی مانند گروه اپوکسی متانو تری متیل (77/17%)، فوران کربوکسی آلدئید (01/17%) و متیل ایندول (02/11%) می­باشد که در ویژگی های آنتی اکسیدانی و ضد میکروبی آن موثر می باشند.
کلیدواژه‌ها
شیره خرمالوی وحشی
آنتی اکسیدانی
ضد باکتریایی
کروماتوگرافی گازی توام با طیف سنجی جرمی (GC-MS)

موضوعات

عنوان مقاله English

Identification of Chemical Compounds and Evaluation of Antioxidant and Antimicrobial Properties of Date Plum (Diospyros Lotus) Syrup

نویسندگان English

Maedeh Kolbadi Nejad
Leila Najafian
Department of Food Science and Technology, Sari Branch, Islamic Azad University, Sari, Iran
چکیده English

Date Plum (Diospyros Lotus) has high health-promoting properties which they are mainly attributed to its available bioactive compounds. In present study, antioxidant and antibacterial properties of the date plum syrup was investigated and the extract compounds identified by Gas Chromatography Mass Spectrometry (GC-MS). Total phenolic and flavonoid contents and antioxidant activity of the extracts were measured. Antimicrobial activity was performed on four bacteria by disc diffusion method. The research results indicated high total phenolic and flavonoid contents of the date plum extracts. Surveying antioxidant properties of date plum extract also implicate its high antioxidant properties. DPPH radical scavenging activity of date plum extract at concentrations of 800, 400, 200, 100 and 50 micrograms/milliliter has been 82% , 61.53%, 53.51%, 32.52%, 18.31%, respectively. In investigation of antibacterial properties, the date plum syrup had high inhibiting effects on growth of different types such as Escherichia coli, Salmonella, Staphylococcus aureus and Bacillus cereus in high dilutions (50 and 100 mg/ml).. The date plum extract includes high amounts of sugar and functional components such as 5,5-Epoxymethano-2,2,6-trimethyl-7-oxabicyclo (17/77%), 2-Furancarboxaldehyde (17/01%) and 1-allyl-3-methylindole-2-carbaldehyde (11/2%).

کلیدواژه‌ها English

Date plum (Diospyros Lotus)
Antioxidant
Antimicrobial
gas chromatography with mass spectrometry (GC-MS)
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