بررسی ویژگی ضدمیکروبی فیلم خوراکی دولایه ژلاتین- صمغ کُندر دارای اسید آسکوربیک و اسانس زوفا (Hyssopus officinalis) بر زمان ماندگاری فیله شترمرغ در دمای یخچال

نویسندگان
1 دانشجوی دکتری میکروبیولوژی مواد غذایی، گروه علوم و صنایع غذایی، دانشکده کشاورزی دانشگاه فردوسی مشهد
2 استاد گروه علوم و صنایع غذایی، دانشکده کشاورزی دانشگاه فردوسی مشهد
چکیده
گوشت شترمرغ از ارزش خوراکی بالایی برخوردار است و یکی از کم­چرب­ترین و سالم­ترین نمونه­های گوشت قرمز در دسترس است. به دلیل آلودگی لاشه در طول زنجیره کشتار، طی زمان نگهداری در اثر رشد میکروارگانیسم­های گوشت فاسد می­شود. زیست­تجزیه­پذیر بودن، خوراکی بودن و کارآمد بودن فیلم­های خوراکی باعث شده است که این فیلم­ها به عنوان جایگزین فیلم­های سنتزی به طور وسیع مورد بررسی قرار گیرند. در این مطالعه فیلم دولایه ژلاتین / کُندر به روش قالب­ریزی و طی دو مرحله تولید شد. در این پژوهش اثر ضدمیکروبی غلظت­های مختلف اسید آسکوربیک (صفر، 1و 2 درصد) و اسانس زوفا (صفر، 75/0و 5/1 درصد) در فیلم خوراکی دولایه ژلاتین/ صمغ کُندر (G/F) در شرایط آزمایشگاهی علیه 4 میکروراگانیسم و همچنین بر ویژگی­های میکروبی و شیمیایی گوشت شترمرغ در دمای یخچال طی 12 روز مورد بررسی قرار گرفت. با تعیین قطر هاله بازداری در روش انتشار دیسک در آگار مشخص شد S.aureus حساس­­ترین و P.aeroginosa مقاوم­ترین باکتری­ها بودند (05/0p<). فیلم­های خوراکی (G/F+2%AA+1.5%HO و G/F+2%AA+1.5%HO) در مقایسه با نمونه کنترل تعداد بار کل باکتریایی، باکتری­های سرمادوست و باکتری­های اسیدلاکتیک را به طور معنی­داری کاهش دادند. از سویی دیگر با افزودن اسانس و اسید، مقدار پراکسید و pH در مقایسه با فیلم خالص به طور معنی­داری کاهش یافت (05/0p<). طبق نتایج به دست آمده فیلم G/F حاوی اسانس و اسیدآسکوربیک با کاهش بار میکروبی گوشت شترمرغ، تخریب بافت و افزایش pH را به تعویق انداخت. بنابراین به لحاظ زیست­محیطی و اقتصادی به عنوان بسته­بندی زیست­تخریب­پذیر و خوراکی مناسب جهت افزایش زمان ماندگاری گوشت شترمرغ در دمای یخچال توصیه می­شود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Survey of antimicrobial gelatin-frankincense (Boswellia carteri) bilayer edible film incorporated with ascorbic acid and Hyssopus officinalis essential oil on ostrich fillets shelf life at refrigerator temperature

نویسندگان English

motahare pirnia 1
Farideh Tabatabaei yazdi 2
Seyed Ali Mortazavi 2
Mohebbat محبی 2
1 PhD student
2 Science committee
چکیده English

Ostrich fillets is highly edible and one of most healthy and the least fat red meat samples. Due to carcass contamination along the slaughter chain, it becomes corrupted by the growth of meat microorganisms during storage. Being biodegradable, edible and efficient have caused edible films to be widely investigated and used as a good replacement for synthetic materials in packaging of food products. In this study a gelatin/frankincense (G/F) bilayer film was produced from gelatin and frankincense monolayers using the casting method in two phases. This research investigated antimicrobial activity of various concentrations of acid ascorbic (0, 1%,2%) and Hyssopus officinalis oil (0, 0.75%, 1.5%) in edible bilayer gelatin/frankincense against S.aureus, B.cereus, E.coli and P.aeroginosa. on the other hand, effect of edible film was evaluated on microbial and chemical properties of ostrich meat for 12 day at refrigerator temperature. Diameter of inhibition zone showed that S.aureus and P.aeroginosa were most sensitive and resistant bacteria respectively. Compared with control sample, two treatments (G/F+2%AA+0.75%HO and G/F+2%AA+1.5%HO) decreased total viable count, psychrophilic and lactic acid bacteria significantly (p<0.05). The result also showed with addition Hyssop oil and ascorbic acid, pH and peroxide values notably reduced compared with pure edible film (without oil and acid)( (p<0.05). The gelatin/frankincense films enriched with essential oil and vitamin C delayed tissue breakdown, and increases the pH by reducing the bacterial growth. Therefore, antimicrobial edible film containing Hyssop oil and vitamin C as an economical and biodegradable coating has a good potential for increasing the shelf-life of ostrich meat at the refrigerator temperature.

کلیدواژه‌ها English

Bi-layer edible film
Frankincense (Boswellia carteri)
Hyssopus officinalis essential oil
Antimicrobial activity
Ostrich fillets
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