بررسی اثر استخراجی التراسوند حمام در شرایط مختلف بر خصوصیات آنتی اکسیدانی عصاره زوفا

نویسندگان
گروه علوم و صنایع غذایی دانشگاه علوم کشاورزی و منابع طبیعی ساری
چکیده
یکی از روشهای نوین استخراج آنتی اکسیدان­های طبیعی از بافت­های گیاهی، استخراج به کمک اولتراسوند است. در این پژوهش از روش استخراج اولتراسوند حمام با حلال اتانول- آب به نسبت­های (۵۰:۵۰ ) و (۸۰:۲۰) در دمای ۳۰ و ۴۰ درجه سانتی گراد و زمان­های ۱۰ و ۲۰ دقیقه برای استخراج عصاره برگ زوفا استفاده شد. میزان کل ترکیبات فنلی و فلاونوئیدی و DPPH و قدرت آنتی­اکسیدانی در سیستم مدل بتاکاروتن – لینولئیک اسید هر یک از عصاره ها به روش اسپکتروفتومتری و فعالیت آنتی اکسیدانی عصاره ها با آزمون شاخص پایداری اکسایشی (OSI) اندازه گیری شد.بر اساس نتایج بدست آمده عصاره (۸۰-۴۰-۲۰) با بالاترین ترکیبات موثره بیشترین میزان ترکیبات فنولی( ۵۳/۵± ۳۸/۱۹۳ بر حسب میلی­گرم گالیک اسید در گرم عصاره )و فلاونوئیدی( ۳۶/۲±۶۳/۴۰ میلی گرم کوئرستین در گرم عصاره) ،و بیشترین مقدار در آزمون­های سنجش آنتی اکسیدانی (مهار رادیکال DPPH و سنجش بی رنگ شدن بتاکاروتن – لینولئیک اسید)و کمترین میزان IC50 را کسب کرد.در نتیجه بهترین عملکرد آنتی اکسیدانی را نشان داد .در تست رنسیمت نیز تیمار (۸۰-۴۰-۲۰) با غلظت ppm200بالاترین پایداری اکسایشی روغن کانولا را نشان داد.بنابراین مناسبترین تیمار برای حصول بهترین نتیجه تیمار (۸۰-۴۰-۲۰)انتخاب شد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigating the effect of extraction of bath ultrasound in different conditions on antioxidant properties of Hyssop (Hyssopus officinalis) extract

نویسندگان English

HADIS AZIMINEZHAD
reza esmaeilzadeh kenari
Zeinab Raftani Amiri
DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY,SARI AGRICULTURAL SCIENCES AND NATURAL RESOURCES UNIVERSITY
چکیده English

One of the new methods for extracting natural antioxidants from plant tissues is ultrasound extraction. In this study, the method of extraction of bath ultrasound with ethanol-water solvent (50:50) and (80:20) at a temperature of 30 and 40 ° C and 10 and 20 minutes was used to extract the extract of the leaves of the zoo. Became The total phenolic and flavonoid compounds, DPPH and antioxidant potency in beta-carotene-linoleic acid model of each extract were measured by spectrophotometric method and antioxidant activity of the extracts by oxidative stability index (OSI). Based on the results, The extract (80-40-20) with the highest effective compounds had the highest phenolic compounds (193.3 ± 5.53 mg / g / g / g extract per gram of extract) and flavonoid (40.63 ± 2.36 mg / 40 mg quercetin in Gram of extract) and the highest amount in antioxidant assays (DPPH radical inhibition and beta-carotene-linoleic acid coloration assay) and the lowest I C50 showed the best antioxidant performance. In the Ransted test, treatment (20-2080) with a concentration of 200 ppm showed the highest oxidative stability of canola oil. Therefore, the most suitable treatment was to obtain the best treatment result (80-40 -20) was selected

کلیدواژه‌ها English

: Hyssop (Hyssopus officinalis)
bath ultrasound
Antioxidant
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