بررسی اثر ضد میکروبی پوشش پروتئین سویا حاوی اسانس گیاه ترخون (Artemisia dracunculud L.) بر فیله مرغ نگهداری شده در دمای یخچال

نویسندگان
1 کارشناس ارشد گروه علوم و صنایع غذایی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
2 استادیار گروه زیست شناسی، واحد تهران شرق ،دانشگاه ازاد اسلامی، تهران، ایران
3 دانشیار گروه علوم و صنایع غذایی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه ازاد اسلامی، تهران، ایران
چکیده
گوشت مرغ یکی از مواد غذایی پر طرفدار و فساد پذیر می باشد. یکی از روشهای موثر جهت افزایش مدت زمان نگهداری گوشت تازه استفاده از پوششهای بسته بندی مناسب حاوی ترکیبات ضد میکروبی طبیعی است. هدف از انجام این تحقیق بررسی اثر ضد میکروبی پوشش پروتئین سویا حاوی اسانس گیاه ترخون بر گوشت مرغ نگهداری شده در یخچال می باشد. اسانس گیاه ترخون با روش تقطیر با آب استخراج شده و خواص ضد میکروبی آن بر باکتریهایStaphylococcus aureus PTCC 1113 ، Salmonella enterica PTCC 1709 ، Escherichia coli PTCC 1399 با روش رقیق سازی بر پایه آگارانجام شده است. پس از آن اثر غلظت های 2/0 و 3/0 درصد (حجمی/حجمی) اسانس محلول در پوشش پروتئین سویا بر روی این باکتری ها بررسی شده و در نهایت اثر ضد میکروبی پوشش پروتئین سویا حاوی اسانس ترخون بر فیله های مرغ نگهداری شده در یخچال بررسی شده و تغییرات ویژگیهای شیمیایی و حسی آن مورد ارزیابی قرار گرفت. بیشترین غلظت بازدارندگی اسانس مربوط به Staphylococcus aureus و کمترین آن مربوط به Escherichia coli و Salmonella enterica بود. شمارش کلی میکروبی برای نمونه تیمار شده با فیلم 3درصد اسانس در طی 12 روز نگهداری در یخچال نسبت به نمونه ی شاهد کاهش معنی داری (05/0>P ) پیدا کرد. بیشترین تأثیر فیلم ضد میکروبی پس از 12 روز نگهداری در یخچال، بر روی نمونه های تلقیح شده با Staphylococcus aureus بود. فیلم پروتئینی 3 درصد اسانس از افزایش pH و عدد پراکسید در طول دوره ی نگهداری در یخچال جلوگیری کرد و مطابق نتایج ارزیابی حسی فیلم حاوی 2درصد اسانس توانست بالاترین امتیاز پذیرش کلی را داشت. اگرچه این خاصیت در اثر اختلاط اسانس با پوشش فیلم کاهش می یابد . همچنین پوشش حاوی اسانس اثرات مثبتی روی خواص شیمیایی و ارگانولپتیکی فیله مرغ گذاشته است.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Antimicrobial effect of soy protein coating containing tarragon essential oil (Artemisia dracunculud L.) on chicken fillet kept at refrigerated temperature

نویسندگان English

niloofar rahmani 1
Akram Sadat Tabatabaee Bafroee 2
anousheh sharifan 3
1 M.Sc Department
2 Assistant Professor
3 Associate Professor
چکیده English



Chicken meat is one of the most popular and perishable foods. One of the effective ways to increase the retention time of fresh chicken meat is the use of appropriate packaging coatings containing natural antimicrobial compounds. The aim of this study was to investigate the antimicrobial effect of soy protein coating containing tarragon essential oil on chicken meat stored in a refrigerator.Tarragon essential oil was extracted by water distillation method and its antimicrobial properties were tested on Staphylococcus aureus PTCC 1113, Salmonella enterica PTCC 1709, Escherichia coli PTCC 1399, and then the effect of concentration 0, 2 and 3% (vol / vol) soluble essential oil in soybean protein coating on these bacteria were studied. Finally, the antimicrobial effect of soy protein content containing tarragon essential oil on chicken fillets kept in the refrigerator was investigated and characteristics of the changes in its chemical and organoleptic properties were evaluated.The highest inhibitory concentration of essential oil was from Staphylococcus aureus, and the lowest was E.coli and Salmonella enterica. The total microbial count for the sample treated with 3% essential oil during the 12 days storage in the refrigerator was significantly lower than the control sample (P <0.05). The greatest effect of antimicrobial films after 12 days of storage in the refrigerator was on inoculated samples of Staphylococcus aureus. Protein film of 3% essential oil prevented the increase of pH and peroxide value during the storage period in the refrigerator. According to the sensory evaluation results, the film containing 2% essential oil obtained the highest overall acceptance rating. Although this property is reduced by the mixing of essential oil with film coating. The essential oil coating also has positive effects on the chemical and organoleptic properties of chicken fillet.

کلیدواژه‌ها English

tarragon essential oil
soy protein isolate film
Staphylococcus aureus
Escherichia coli
Salmonella enterica
[1] Azerdo,H.M.C. 2013. Antimicrobial nanostructures in food packaging, trends in food science & technology,30(1):56-69
[2] Rokni, N. 1394 . Principles of food hygiene. Ninth Edition. Tehran University Press. Pages 27-29.
[3] Zargari, A . 1375 . Medicinal plants. Sixth Edition, Institute. University of Tehran Press. Volume Three of Pages 117-121.
[4] Briozzo, J., Núncez, L., Chirife, J., Herszage, L., D'aquino, M.1989. Antimicrobial activity of clove oil dispersed in a concentrated sugar solution”, Journal of Applied Bacteriology, Vol. 66, No. 1, pp. 69-75.
[5] Sivarooban.T,Navam.S. Hettiarachchy, and Michel.G,Johnson. 2006. Inhibition of Listeria monocytogenes by Nisin Combined with Grape Seed Extract or Green Tea Extract in Soy Protein FilmCoated on Turkey Frankfurters. Journal of food science—Vol. 71.
[6] Mahon, C.R. and Manuselis, G. 1995. Textbook of diagnostic microbiology. W.B. Saunders Company, Tokyo. pp. 121-122.
[7] Clinical and laboratory standards Institute( formely NCCLS),2006. Method fot antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; approved guideline .M45-A. clinical and Laboratory Standards institute,wayne,PA.
[8] Magaldi, S. and Camero, T. 1997. Susceptibilidad de Candida albicans ‘in vitro’mediante los pozos de difusión. Boletín Venezolano de Infectología, 7, pp.5-8.
[9] No name. 1393 . Food Chain Microbiology - A Comprehensive Approach to Counting Microorganisms - Part 2- Colony Counting at 30 ° C Using Surface Culture Method. Iranian Institute of Standards and Industrial Research, National Iranian Standard, no 2-5272
[10] No name. 1384 . Microbiology of food and feed - Counting of coagulase-positive Staphylococcus aureus (Staphylococcus aureus and other species) -Test method-Part I: Method of use of culture medium-Parkragar. Iranian Institute of Standards and Industrial Research, National Iranian Standard, no 1-6806
[11] No name. 1393. Food and Feed Microbiology - A Comprehensive Method for Searching and Identifying Salmonella Species. Iranian Institute of Standards and Industrial Research, National Iranian Standard, No. 1810.
[12] No name. 1384. Microbiology of food and feed - The method of searching and counting Escherichia coli using the most probable method. Iranian Institute of Standards and Industrial Research, National Iranian Standard, No. 2946.
[13] Molaei Aghaei, E.Kamkar, A. Khangari, A. Oladi, M. 1396. Effect of Chitosan Packaging with Cumin Essential Oil on Chemical and Microbial Properties of Chicken Fillet. Journal of Fasa University of Medical Sciences. Seventh year. No. 1. 115-104 pages
[14] Sharifan, A. 1384 . The Effect of Essential Oil of Thyme and Shirazi Plants on Staphylococcus aureus Bacteria. PhD thesis in Agriculture-Food Science and Technology
[15] Tabatabaei Yazdi, F., Alizadeh Behbahani, B., Shahidi, Fakhri., Mortazavi, A. Mohebi. V., Mohabbat. 1396 . Investigation of chemical composition and antimicrobial activity of Tarragon essential oil on some pathogenic bacteria in vitro. Qom University of Medical Sciences Journal. Eleventh Edition, No. 9, pp. 51-42.
[16] Sharafati Chaleshtori, R., Rokni,N,. Razavilar, V., Rafieian Kopaei, M. 2012 .The Evaluation of the Antibacterial and Antioxidant Activity of Tarragon. Jundishapur J Microbiol. 2013;6(9):e7877
[17] Marino, M., Bersani, C. and Comi, G.1999. Antimicrobial activity of the essential oils of Thymus vulgaris L. measured using a bioimpedometric method. Journal of food protection, 62(9), pp.1017-1023.
[18] Mohsenzadeh M .2007. Evaluation of antibacterial activity of . selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium. Pak J Bio; 10: 3693-3697.
[19] Bonyadian M, Moshtaghi H .2008.Bacteriocidal Activity of Some Plants Essential Oils Against Bacillus cereus,Salmonella typhimurium, Listeria monocytogenes and Yersinia enterocolitica. Res J Microbiol. ;3(11):648–653.
[20] Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A. and Yildirim, A.2005. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils. Journal of agricultural and food chemistry, 53(24), pp.9452-9458.
[21] Sharafati-Chaleshtoria, R. Roknib, N. Rafieian-Kopaeic, M. Dreesd, F. Sharafati-chaleshtoric, A and Salehic,E. 2014. Antioxidant and Antibacterial Activity of Basil (Ocimum basilicum L.) Essential Oil in Beef Burger. Journal of food science. vol. 26 .
[22] Safaei, M., Hosseini, E. And Shabani, S. 1394. First International Conference on Iranian Food Industries, Tehran, Iran Development Conferences Center
[23] Appendini, P. and Hotchkiss, J.H. 2002. Review of antimicrobial food packaging. Innovative Food Science & Emerging Technologies, 3(2), pp.113-126..
[24] Jouki, M. and Khazaei, N. 2011. Effects of storage time on some characteristics of packed camel meat in low temperature. Int J Anim Vet Adv, 3(6), pp.460-64.
[25] Khanjari, A., Karabagias, I.K., Kontominas, M.G. 2013. Combined effect of N,O-carboxymethyl chitosan and oregano essential oil to extend shelf life and control Listeria monocytogenes in raw chicken meat fillets, Food Science and Technology, 53: 94-99
[26] Ranjbarian, S., Rezazad Bari, M., Diamond, H. And Amiri, S. 1396 . The effect of film and coating of sodium caseinate active nanocomposite containing cinnamon essential oil on enhancing shelf life of chicken breast. Journal of Food Science and Technology, Volume 14, Number 71, pp. 184-171.
[27] Bazargani-Gilani,B., Aliakbarlu, J.,Tajik, H. 2015. Effect of pomegranate juice dipping and chitosan coating enriched with Zataria multiflora Boiss essential oil on the shelf-life of chicken meat during refrigerated storage. Elsevier Ltd All rights reserved
[28] Brannan, R.G.2009. Effect of grape seed extract on descriptive sensory analysis of ground chicken during refrigerated storage. Meat science, 81(4), pp.589-595.
[29] Hosseini, M.H., Razavi, S.H. and Mousavi, M.A. 2009 . Antimicrobial, physical and mechanical properties of chitosan‐based films incorporated with thyme, clove and cinnamon essential oils. Journal of Food Processing and Preservation, 33(6), pp.727-743.
[30] Ahmad, M., Benjakul, S., Prodpran, T. and Agustini, T.W. 2012. Physico-mechanical and antimicrobial properties of gelatin film from the skin of unicorn leatherjacket incorporated with essential oils. Food Hydrocolloids, 28(1), pp.189-199.