بررسی فعالیت ضدمیکروبی اسانس رازیانه بر تعدادی از ریزاندامگان عامل عفونت و مسمومیت غذایی و برهمکنش آن با آنتی‌بیوتیک کانامایسین

نویسندگان
1 دانشگاه علوم کشاورزی و منابع طبیعی خوزستان
2 دانشگاه فردوسی مشهد
چکیده
اخیرا، استفاده از نگهدارنده­های شیمیایی و سنتزی برای کنترل رشد ریزاندامگان بیماری­زا به طور چشم­گیری کاهش یافته است. امروزه، اسانس­های روغنی به عنوان یک نگهدارنده طبیعی در نظر گرفته می­شوند. رازیانه یکی از گیاهان دارویی معطر می­باشد که می­توان از آن به عنوان یک نگهدارنده طبیعی استفاده کرد. در این پژوهش، برای بررسی فعالیت ضد­میکروبی اسانس رازیانه بر تعدادی از ریزاندامگان عامل عفونت و مسمومیت غذایی (اشرشیا کلی، استافیلوکوکوس اورئوس و کاندیدا آلبیکنس) از روش­های دیسک دیفیوژن آگار (کربی-بوئر)، چاهک آگار، تعیین حداقل غلظت مهارکنندگی و تعیین حداقل غلظت کشندگی استفاده شد. برهمکنش اسانس رازیانه با آنتی­بیوتیک کانامایسین نیز بررسی شد. نتایج آزمون­های دیسک دیفیوژن اگار و چاهک آگار نشان داد که حساس­ترین باکتری با بیشترین قطر هاله عدم رشد نسبت به سایر ریزاندامگان بیماری­زا، استافیلوکوکوس اورئوس بود. برهمکنش اسانس رازیانه با آنتی­بیوتیک­ کانامایسین برای باکتری­های استافیلوکوکوس اورئوس و اشرشیا کلی به صورت اثر سینرژیستی مشاهده شد. حداقل غلظت مهارکنندگی اسانس رازیانه برای استافیلوکوکوس اورئوس، اشرشیا کلی و کاندیدا آلبیکنس به ترتیب 25، 50 و 100 میلی­گرم بر میلی­لیتر بود. به طور کلی می­توان بیان کرد که اسانس رازیانه در شرایط برون تنی به خوبی توانست از رشد اشرشیا کلی، استافیلوکوکوس اورئوس و کاندیدا آلبیکنس جلوگیری نماید.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigation of antimicrobial activity of Fennel essential oil on some pathogenic microorganisms causing infection and food poisoning and its interaction with kanamycin antibiotic

نویسندگان English

Behrooz Alizadeh behbahani 1
Mohammad Noshad 1
Fereshteh Falah 2
1 Agricultural Sciences and Natural Resources University of Khuzestan
2 Ferdowsi University of Mashhad
چکیده English

Recently, the use of chemical and synthetic preservatives for the control of microbial pathogenesis growth has been remarkably decreased. Today, essential oils (EOs) have been considered to be natural preservatives. Fennel is among the aromatic medicinal plant that can be used as a natural preservative. In this study, to investigate the antimicrobial activity of fennel essential oil on some pathogenic microorganisms causing infection and food poisoning (Escherichia coli, Staphylococcus aureus and Candida albicans) were used disk diffusion agar (Kirby-Bauer), well diffusion agar, minimum inhibitory concentration and minimum bactericidal/fungicidal concentration methods. The interaction of fennel essential oil (FEO) with kanamycin antibiotic was investigated. The results disc diffusion agar and well diffusion agar antimicrobial assays showed that S. aureus was the most sensitive bacteria with the highest inhibition zone compared to the other microorganisms. The effects of interaction of FEO with kanamycin antibiotic on Escherichia coli, Staphylococcus aureus showed synergistic status. In general, FEO was able to prevent the growth of Escherichia coli, Staphylococcus aureus and Candida albicans “in vitro”.

کلیدواژه‌ها English

Fennel essential oil
Kanamycin antibiotic
In vitro
Synthetic preservatives
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