استخراج اسانس از بذر رازیانه با استفاده از آب فوق داغ و مقایسه آن با تقطیر آبی

نویسندگان
سینا صادق موسوی 1
آرزو دستپاک 2
1 فارغ التحصیل مهندسی شیمی
2 استادیار گروه زیست شناسی دانشگاه آزاد اسلامی واحد تهران مرکزی
چکیده
چکیده
سابقه و هدف: استخراج به کمک آب فوق داغ به دلیل داشتن مزایایی از قبیل سرعت و بازدهی بالا، کاهش مقدار حلال­های آلی مورد استفاده نسبت به روش­های سنتی و همچنین به دلیل سازگار بودن با محیط زیست مورد توجه قرار گرفته است.

مواد و روش­ها: در این مطالعه استخراج اسانس از بذر گیاه رازیانه (Foeniculum vulgare Mill.) با استفاده از روش استخراج با آب فوق داغ انجام شد و با روش تقطیر با آب مقایسه قرار گردید. ترانس آنتول به عنوان ترکیب اصلی، هدف انجام آزمایش­های بعدی قرار گرفت. جداسازی و تشخیص ترانس آنتول استخراج شده به وسیله GC-FID و GC/MS انجام شد. تاثیر پارامترهای دما، متوسط اندازه ذره و شدت جریان بر روی میزان استخراج در روش آب فوق داغ بررسی شدند. به منظور تسهیل روند آزمایش­ها و مقایسه پارامترهای موثر از روش طراحی آزمایش تاگوچی استفاده شد. نسبت مساحت ترانس آنتول به استاندارد داخلی حاصل از کروماتوگرافی گازی به عنوان معیار ارزیابی تاثیر پارامترها و روش­های استخراج اسانس از دانه رازیانه محاسبه شد.

یافته­ها: نتایج آنالیز واریانس نشان داد که دما و متوسط اندازه ذره تاثیر قابل توجهی بر میزان استخراج ترانس آنتول دارند. بیشترین نسبت سطح ترانس آنتول به استاندارد داخلی در دمای 175 درجه سانتی­گراد، متوسط اندازه 398/0 میلی­متر و شدت جریان 3 میلی­لیتر در دقیقه به دست آمد.

نتیجه­گیری: نتایج این مطالعه نشان داد که استخراج با آب فوق داغ در مقایسه با روش تقطیر با آب یک شیوه سریع با بازدهی بالا به منظور استخراج ترانس آنتول به عنوان یک ترکیب اکسیژن­دار با ارزش می­باشد.
کلیدواژه‌ها
واژه های کلیدی: استخراج با آب فوق داغ
تقطیر با آب
ترانس آنتول
رازیانه

موضوعات

عنوان مقاله English

Subcritical water extraction of fennel seeds essential oil and comparison with hydrodistillation

نویسندگان English

Sina Sadeghmousavi 1
arezoo dastpak 2
1 graduated of chemical engineering
2 Assistant professor of Biology
چکیده English

Abstract

Background and purpose: Subcritical water extraction has been considered due to advantages such as high speed, high efficiency and reduced amount of organic solvents used in the traditional way as well as due to its compatibility with the environment.

Materials and Methods: In this study, the essences extraction from fennel seeds was performed by using subcritical water extraction and was compared with hydrodistillation method. Trans-anethole as the main combination has been carried out as a main goal of next experiments. Separating and diagnosis of the extracted trans-anethole were performed by GC-FID and GC / MS. The effect of temperature parameters, Average of particle size and flow intensity was studied on the extraction rate in subcritical water method. Taguchi experimental design was used to facilitate the process of testing and comparing the effective parameters. The ratio of the area of trans-anethole to the obtained internal standard from gas chromatography was calculated as evaluated criteria of parameters and extraction methods of essence from fennel seeds.

Results: The results of Analysis of variance showed that the temperature and, Average of particle size have a significant effect on the amount of trans-anethole extraction. The highest ratio of trans-anethole to internal standard was obtained at 175 ° C, an average value of 0.398 mm, and a flow rate of 3 ml /min.

Conclusion: The results of this study showed that the extraction by subcritical water in compare with hydro distillation method is a fast-high-efficiency method for extraction of trans-anethole as a valuable oxygen-containing compound.

کلیدواژه‌ها English

Keywords: Extraction by Sub-Critical water
Hydro distillation
Trans -Anethole
Fennel
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