بهبود دسترسی زیستی پلی‌فنل‌های پوست انار با تشکیل کمپلکس الحاقی با بتاسیکلودکسترین (β-CD)

نویسندگان
1 دانشجوی دکتری علوم و صنایع غذایی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
2 استادیار گروه علوم و صنایع غذایی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
3 دانشیار و عضو هیئت علمی گروه علوم و صنایع غذایی دانشگاه ارومیه
چکیده
با توجه به مسائل زیست محیطی، ایمنی، سمیت و ویژگی­های ارگانولپتیکی نامطلوب عصاره­های استخراجی با حلال­های آلی جهت استفاده در سیستم­های غذایی، جایگزینی این روش­ها با روش­های استخراج آبی با راندمان بالای استخراج ترکیبات زیست فعال بیش از پیش مورد نیاز است. در مطالعه حاضر کارایی کاربرد همزمان محلول آبی بتاسیکلودکسترین (β-CD) و روش اولتراسوند جهت استخراج ترکیبات پلی­فنلی از پوست انار در مقایسه با روش استخراج آبی مورد بررسی قرار گرفت. روش سطح پاسخ جهت بهینه­سازی شرایط استخراج انجام گرفت. آزمون­ها بر مبنای طرح Box-Behnken با سه متغیر مستقل شامل دما (۷۰-۳۰ درجه سانتی­گراد)، زمان (۴۰-۱۰ دقیقه) و غلظت محلول β-CD (۸/۱-۰ درصد) طراحی شدند. شرایط بهینه استخراج، دمای ۷۶/۵۵ درجه سانتی­گراد، زمان ۳۸/۱۵ دقیقه و غلظت ۸/۱ درصد از β-CD تعیین شد که تحت این شرایط بیشترین میزان ترکیبات پلی­فنلی غیرفلاونونیدی ۷۷/۷۵ (mgGAE/gDW) و بازده استخراج ۸۱/۴۲ درصد بود. همچنین نتیجه نتایج آزمون­های DSC، FTIR و SEM نیز صحت تشکیل کمپلکس الحاقی (Inclusion complex) مابین ترکیبات پلی­فنلی پوست انار و بتاسیکلودکسنرین را تایید نمود. نتایج تحقیق حاضر نشان داد استفاده از محلول β-CD به همراه فرایند اولتراسوند به عنوان یک روش ایمن و کارا می­تواند جایگزین روش­های دیگر استخراج جهت استخراج ترکیباتی با ارزش تغذیه­ای بالا شود.
کلیدواژه‌ها

عنوان مقاله English

Improving bioavailability of pomegranate peel polyphenols by forming an inclusion complex with β-cyclodextrin (β-CD)

نویسندگان English

Sara Kalantari 1
Leila Roufegarinejad 2
sajad pirsa 3
Mehdi Gharekhani 2
1 Department of Food Sciences, Tabriz branch, Islamic Azad University, Tabriz, Iran
2 Department of Food Sciences, Tabriz branch, Islamic Azad University, Tabriz, Iran
3 Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده English

Due to the use of organic solvents is associated with environmental pollution, toxicological and safety concerns, alternative extraction methods have been investigated. In this study, the efficacy of application of β-Cyclodextrin (β-CD) and ultrasound-assisted extraction (UAE) as an eco-friendly, cost-effective and safe method for extraction of bioactive compounds from pomegranate peel compared to the aqueous extraction method was studied. The response surface method (RSM) was used to optimize extraction conditions. Designed experiments were done based on the Box-Behnken design with three independent variables, including temperature (30, 50 and 70 °C), time (10, 25 and 40 min) and concentration of β-CD solution (0, 0.9 and 1.8 %). The optimum extracting temperature and time and β-CD concentration were 55.7°C, 15.38 min, 1.8% respectively. In the optimum condition, the following characteristics of extracted bioactive compounds were obtained: Non-flavonoid phenols 75.77 (mgGAE/gDW( and extraction efficiency was 42.81%. Inclusion complex formation was confirmed by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR) and SEM. As a result of this study, aqueous β-CD and UAE as a safe method can be replaced with other extraction methods to extract compounds with high nutritional value.

کلیدواژه‌ها English

β-Cyclodextrine
Ultrasound
pomegranate peel
Bioactive compounds
Non-flavonoid phenols
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