شناسایی ترکیبات شیمیایی و فعالیت ضدباکتریایی اسانس ریحان سبز و برهمکنش آن با آنتی بیوتیک های تتراسایکلین و کلرامفنیکل بر تعدادی از ریزاندامگان عامل عفونت و مسمومیت غذایی

نویسندگان
حسن برزگر 1
بهروز علیزاده بهبهانی 2
محمدامین مهرنیا 2
1 دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده
در این پژوهش، اثر ضدمیکروبی اسانس ریحان سبز به روش­های متنوع کیفی و کمی (دیسک دیفیوژن، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی) و همچنین برهمکنش (اینترکشن) آن با آنتی­بیوتیک­های تتراسایکلین و کلرامفنیکل بر باکتری­های لیستریا اینوکوا، استافیلوکوکوس اورئوس، باسیلوس سرئوس، سودوموناس ائروژینوزا و اشرشیا کلی مورد ارزیابی قرار گرفت. ترکیبات شیمیایی، قدرت آنتی­اکسیدانی و فنل کل اسانس ریحان سبز نیز تعیین شد. نتایج نشان داد که اسانس ریحان سبز حاوی 31 ترکیب بود. استراگول با 97/49 درصد بیشترین جزء اسانس بود. قدرت آنتی­اکسیدانی اسانس ریحان سبز براساس فعالیت مهار رادیکال پایدار 2و2- دی فنیل1- پیکریل هیدرازیل برابر با 70 درصد بود. میزان فنل کل اسانس ریحان سبز 50/0± 05/29 میلی­گرم گالیک اسید بر گرم بود. بیشترین و کم­ترین فعالیت ضدمیکروبی اسانس ریحان سبز با قطر 95/21 و 10 میلی­متر به ترتیب برای باکتری­های باسیلوس سرئوس و اشرشیا کلی مشاهده شد. در حالت ترکیبی (اینترکشن) اسانس ریحان سبز با آنتی­بیوتیک­های تتراسایکلین و کلرامفنیکل بر باکتری­های گرم منفی حالت سینرژیستی مشاهده شد. حداقل غلظت بازدارندگی اسانس برای باکتری­های لیستریا اینوکوا، استافیلوکوکوس اورئوس، باسیلوس سرئوس، سودوموناس ائروژینوزا و اشرشیا کلی به ترتیب 5/12، 25/6، 25/6، 200 و 200 میلی­گرم بر میلی­لیتر بود. حداقل غلظت کشندگی اسانس ریحان سبز برای باکتری­های مذکور نیز به ترتیب 50، 5/12، 5/12، 400 و 400 میلی­گرم بر میلی­لیتر بود.
کلیدواژه‌ها
ریحان سبز
فنل کل
رقیق‌سازی در مایع
تتراسایکلین
کلرامفنیکل

موضوعات

عنوان مقاله English

Identification of the chemical compounds and antibacterial activity of Ocimum basilicum essential oil and the effects of its interaction with tetracycline and chloramphenicol antibiotics on some pathogenic microorganisms causing infection and food poisoning

نویسندگان English

Hassan Barzegar 1
Behrooz Alizadeh behbahani 2
Mohammad Amin Mehrnia 2
1 Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

In this study, the antimicrobial effect of Ocimum basilicum essential oil with different qualitative and quantitative methods (disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration) as well as the effects of its interaction with tetracycline and chloramphenicol antibiotics on Listeria innocua, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli were evaluated. The chemical compounds, antioxidant activity and total phenolic content of Ocimum basilicum essential oil were measured. The results showed that Ocimum basilicum essential oil contained 31 compounds. Estragole was the major constituent of the essential oil with a percentage of 30.75. The antioxidant activity of Ocimum basilicum essential oil was 70% based on free radical scavenging capacity (DPPH). The total phenolic content of the essential oil was 29.05±0.50 mg GAE/g. The most sensitive and the most resistant bacteria with diameter inhibition zone 21.95 mm and 10.00 mm were Bacillus cereus and Escherichia coli respectively. The effects of interaction of Ocimum basilicum essential oil with tetracycline and chloramphenicol antibiotics on gram negative bacteria showed synergistic status. The minimum inhibitory concentration of the essential oil was equal to 12.5, 6.25, 6.25, 200 and 200 mg/ml for Listeria innocua, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli respectively. The minimum bactericidal concentration of Ocimum basilicum essential oil was equal to 50, 12. 5, 12.5, 400 and 400 mg/ml for bacteria respectively.

کلیدواژه‌ها English

Ocimum basilicum
Total phenolic content
Microdilution broth
Tetracycline
Chloramphenicol
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