تأثیر پوشش‌ خوراکی بر پایه کنسانتره پروتئین آب‌پنیر حاوی ناتامایسین یا کونژوگه آنزیم لیزوزیم – گزانتان بر خصوصیات میکروبی پنیر سفید فراپالایشی

نویسندگان
1 دانشگاه آزاد اسلامی واحد ماکو
2 دانشگاه تبریز
3 شرکت پگاه تبریز
4 دانشگاه یوزونجوییل ترکیه
چکیده
در این تحقیق تأثیر پوشش‌های خوراکی بر پایه پروتئین آب‌پنیر حاوی غلظت‌های مختلف ناتامایسین و کونژوگه لیزوزیم- صمغ گزانتان بر خصوصیات میکروبی پنیر سفید فراپالایش موردبررسی قرار گرفت. برای این منظور اشریشیاکلی O157:H7 (به‌عنوان یک شاخص برای باکتری‌های گرم منفی و مقاوم در برابر پاستوریزاسیون تجاری)، استافیلوکوکوس اورئوس (به‌عنوان شاخص باکتری‌های گرم مثبت) و کپک پنی‌سیلیوم کریزوژنوم بر روی سطح پنیر سفید فراپالایشی (UF) تلقیح شد و خواص میکروبی پنیر در طول 28 روز دوره نگهداری ارزیابی شد. نتایج نشان داد که همه تیمارهای پوشش خوراکی به‌طور معنی‌داری رشد کپک پنی‌سیلیوم کریزوژنوم را کاهش داد. پوشش‌های حاوی ناتامایسین در کاهش جمعیت این کپک مؤثرتر از پوشش‌های حاوی لیزوزیم-گزانتان بودند. پوشش حاوی با غلظت 600 پی‌پی‌ام باکتری اشریشیا‌کلی O157:H7 به میزان 09/2 سیکل لگاریتمی در مقایسه با نمونه‌های بدون پوشش کاهش داد. همچنین رشد استافیلوکوکوس اورئوس در تمامی نمونه‌های تیمار شده با آنزیم لیزوزیم-گزانتان، کمتر از نمونه کنترل بود. کمترین میزان رشد باکتری استافیلوکوکوس اورئوس در نمونه پوشش داده‌شده حاوی 600 پی‌پی‌ام لیزوزم-گزانتان در روز 28 مشاهده شد که جمعیت میکروبی آن 60/2 لگاریتم بود. برخلاف سایر تیمارها، روند رشد باکتری استافیلوکوکوس اورئوس در نمونه پوشش‌ داده‌شده با پروتئین آب‌پنیر حاوی 600 پی‌پی‌ام آنزیم لیزوزیم-گزانتان تا روز 28 ام، نزولی بود به‌طوری‌که جمعیت آن از 41/5 لگاریتم در روز نخست، به 60/2 لگاریتم در روز 28 ام کاهش یافت. نتایج پژوهش حاضر نشان داد که استفاده از پوشش‌های خوراکی بر پایه پروتئین آب‌پنیر به‌عنوان حامل ناتامایسین و کونژوگه لیزوزیم گزانتان در غلظت بهینه می‌تواند جهت افزایش کیفیت میکروبی پنیر فراپالایشی مورداستفاده قرار گیرد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

The Effect of Whey Protein Concentrate Based Edible Coatings Containing Natamycin or Lysozyme-Xanthan conjugate on Microbial properties of ultrafiltrated white cheese

نویسندگان English

Abbas Jalilzadeh 1
Javad Hesari 2
Seyed Hadi Peighambardoust 2
Hosein Jodeiri 3
Issa Javidipoor 4
1 Islamic Azad Univrsity Maku Branch
2 University of Tabriz
3 Pegah Co.
4 Yüzüncü Yıl University
چکیده English

In this research, the effects of whey protein concentrate based edible coatings containing different concentrations of natamycin and lysozyme–xanthan gum conjugate were investigated. For this purpose, Escherichia coli O157:H7 (as an indicator for gram negative bacteria and also resistant to commercial pasteurization), Staphylococcus aureus (as an indicator of gram-positive bacteria), and Penicillium chrysogenum were inoculated to ultrafiltrated white cheese surface and the microbial properties of cheese samples were evaluated during 28 days storing. The results showed that all coated treatments significantly reduced the growth of Penicillium chrysogenum. Natamycin-containing coatings have been more effective in reducing the mold population than lysozyme-xanthan-containing coatings. Coated samples containing 600 ppm lysozyme-xanthan reduced E. coli O157: H7 growth 2.09 log compared to control samples. Also, the growth rates of Staphylococcus aureus were lower in all samples treated with lysozyme-xanthan than control sample. The lowest growth rate of Staphylococcus aureus was observed in the coated sample containing 600 ppm lysozyme-xanthan on 28th day, with a microbial population of 2.60 logarithms. Unlike other treatments, the growth rate of Staphylococcus aureus in the sample coated containing 600 ppm lysozyme-xanthan was descending over 28 days. The results of this study showed that whey protein based edible coating can be used as a carrier of natamycin and lysozyme-xanthan in optimal concentration, for increasing the microbial quality of UF cheese.

کلیدواژه‌ها English

Edible coating Escherichia coli O157: H7 Lysozyme-Xanthan conjugate Staphylococcus aureus
Ultrafiltraed white cheese
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