بهینه سازی آبکافت آنزیمی ضایعات حاصل ازکشتار مرغ به روش سطح پاسخ

In this study Response Surface Methodology (RSM) was employed to investigate the effects of different operating conditions on the hydrolysation process of poultry by-products protein by the alcalase. Central Composite Design (CCD) by 5-level and 3-factor was used for the optimization of the hydrolysate production and to evaluate the effects and interactions of process variables: time (minute), temperature (C^◦) and enzyme to substrate ratio on the response. The response was included degree of hydrolysation (%). The mathematical model showed good fit with the experimental data, since the R² of 0.96 indicated that 96% of the variability within the range of values studied could be explained by the model. Furthermore lake of fit of the model was not significant (p<0.05). The optimum conditions for production of poultry by-products protein hydrolysate by 15.24% degree of hydrolysis were established as an enzyme activity to substrate ratio 0.07 AU/g protein; time 127.69 minute, and temperature 52.51°C. According to amino acid analysis and chemical score, methionine and histidine was the limiting amino acids in the hydrolysate. The poultry by-products protein hydrolysate tends to be a potential source in balanced fish diets and functional additive in food industries.