Volume 20, Issue 142 (2023)                   FSCT 2023, 20(142): 161-170 | Back to browse issues page


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Noshad M, Alizadeh behbahani B. Antimicrobial activity of Datura stramonium ethanolic extract on some pathogenic bacteria causing infection and food poisoning in vitro. FSCT 2023; 20 (142) :161-170
URL: http://fsct.modares.ac.ir/article-7-70512-en.html
1- Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran. , mo.noshad@gmail.com
2- Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
Abstract:   (728 Views)
Datura stramonium is well known as a valuable drug in many diseases including inflammation, wounds, fever and toothache, and its extract has significant antimicrobial activity. Therefore, this study was performed to isolate the ethanolic extract of D. stramonium leaves and to investigate its antimicrobial activity. In this study, D. stramonium extract was obtained using the maceration method. Antimicrobial methods of disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration were used to determine the antibacterial activity of ethanolic extract of D. stramonium leaves against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Bacillus cereus. The antimicrobial activity of the extract was concentration dependent and the highest diameter of growth inhibition zone was observed at a concentration of 60 mg/ml of the extract. According to disk diffusion agar and well diffusion agar tests, S. aureus and S. typhimurium had the highest and lowest diameter of growth inhibition zones, respectively. The minimum inhibitory concentrations for E. coli, S. typhimurium, S. aureus and B. cereus were 16, 16, 8 and 8 mg/ml, respectively, and the minimum bactericidal concentrations for these bacteria were 256, 256, 64, and 128 mg/ml, respectively. In general, Gram-positive bacteria were more sensitive to the extract than Gram-negative types. Ethanolic extract of D. stramonium leaves can be used as a natural antimicrobial agent to prevent the growth of pathogenic bacteria and treat related diseases.
 
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Article Type: Original Research | Subject: Essences and extracts
Received: 2023/07/15 | Accepted: 2023/08/22 | Published: 2023/10/2

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