بررسی گروه‌های عاملی، ترکیبات فنولی و فلاوونوئیدی، فعالیت آنتی‌اکسیدانی و ضد‌میکروبی اسانس اسطوخودوس لارستانی: یک مطالعه آزمایشگاهی

نویسندگان
مینا علادی 1
محمدامین مهرنیا 2
حسن برزگر 3
بهروز علیزاده بهبهانی 1
1 علوم کشاورزی و منابع طبیعی خوزستان
2 دانشگاه علوم کشاورزی و منابع طبیعی خوزستان
3 علوم کشاورزی و منایع طبیعی خوزستان
10.52547/fsct.18.119.61
چکیده
هدف از این پژوهش تعیین گروه­های عاملی و شناسایی کیفی، میزان ترکیبات فنولی و فلاوونوئیدی، فعالیت آنتی­اکسیدانی و ضد­میکروبی اسانس اسطوخودوس لارستانی بود. گروه­های عاملی اسانس به وسیله طیف‌ سنجی تبدیل فوریه فروسرخ (FTIR) در محدوده طول موج cm-1 4000–500 تعیین گردید. میزان فنول و فلاوونوئید کل به ترتیب به روش­های فولین سیوکالچو و رنگ سنجی آلومینیوم تری کلراید اندازه گیری شد. فعالیت آنتی­اکسیدانی نیز به روش­های مهار رادیکال آزاد DPPH و ABTS تعیین گردید. پیک­های مشاهده شده در اسانس اسطوخودوس لارستانی مؤید حضور گروه­های عاملیO-H ،C-H ،C=O ،C=C ،C-C و C-O ترکیبات زیست فعال بود. مقدار فنول و فلاوونوئید کل اسانس به ترتیب برابر باmg GAE/g 68/0±60/68 و mg QE/g 52/0±10/19 تعیین شد. فعالیت آنتی­اکسیدانی نیز بر اساس درصد مهارکنندگی رادیکال آزاد DPPH و ABTS به ترتیب برابر با 63/0±50/59 و 53/0±68/67 به دست آمد. فعالیت ضد­میکروبی اسانس نیز به چهار روش دیسک دیفیوژن، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی مورد ارزیابی قرار گرفت. نتایج حاصل از ازریابی فعالیت ضد­میکروبی به دو روش دیسک دیفیوژن و چاهک آگار نشان داد که باکتری گرم مثبت استافیلوکوکوس اورئوس حساس­ترین سویه به اسانس اسطوخودوس لارستانی بود. حداقل غلظت مهارکنندگی اسانس برای باکتری­های سالمونلا تیفی، سودوموناس آئروژینوزا، لیستریا اینوکوآ، استافیلوکوکوس اورئوس و اشرشیاکلی برابر با mg/ml 125/3 و برای باسیلوس سرئوس برابر باmg/ml 25/6 بود. حداقل غلظت کشندگی برای باکتری­های لیستریا اینوکوا، سودوموناس آئروژینوزا و سالمونلا تیفی به ترتیب برابر با mg/ml 100، 200 و 400 و برای سایر باکتری­ها (استافیلوکوکوس اورئوس، باسیلوس سرئوس و اشرشیاکلی) بیشتر از mg/ml400 بود.
کلیدواژه‌ها
اسانس روغنی
اسطوخودوس لارستانی
فعالیت ضد‌میکروبی
گروه‌های عاملی

موضوعات

عنوان مقاله English

Investigation of functional groups, phenolic and flavonoid compounds, antioxidant and antimicrobial activity of Lavandula stricta essential oil: An “in vitro” study

نویسندگان English

mina aladi 1
Mohammad Amin Mehrnia 2
Hassan Barzegar 3
behrooz Alizadeh behbahani 1
1 Agricultural Sciences and Natural Resources University of Khuzestan
2 Agricultural Sciences and Natural Resources University of Khuzestan
3 Agricultural Sciences and Natural Resources University of Khuzestan
چکیده English

The aim of this study was to determine the functional groups and qualitative identification, the amount of phenolic and flavonoid compounds, antioxidant and antimicrobial activity of Lavandula stricta essential oil. Essential factor groups were determined by Fourier transform infrared (FTIR) spectroscopy in the wavelength range of 500–4000 cm-1. Total phenols and flavonoids were measured by Folin-Ciocalteu and aluminum trichloride colorimetry, respectively. Antioxidant activity was also determined by two methods of free radical scavenging DPPH and ABTS. The peaks observed in Lavandula stricta essential oil confirmed the presence of O-H, C-H, C=O, C=C, C-C and C-O functional groups of bioactive compounds. The amount of phenol and flavonoids in total essential oil was 68.60±0.68 mg GAE/g and 19.10±0.52 mg QE/g, respectively. Antioxidant activity was also obtained based on the percentage of free radical scavenging DPPH and ABTS equal to 59.50±0.63 and 67.68±0.53, respectively. The antimicrobial activity of essential oil was evaluated by four methods: disk diffusion, agar well, minimum inhibitory concentration and minimum bactericidal concentration. The results of measuring the antimicrobial activity of essential oil by disk diffusion and agar well showed that Gram-positive bacterium Staphylococcus aureus is the most sensitive strain to Lavandula stricta essential oil. The minimum inhibitory concentration of essential oil for Salmonella typhi, Pseudomonas aeruginosa, Listeria innocua, Staphylococcus aureus and Escherichia coli was equal to 3.125 mg/ml and for Bacillus cereus it was equal to 6.25 mg/ml. The minimum bactericidal concentrations for Listeria innocua, Pseudomonas aeruginosa and Salmonella typhi was equal to 100, 200 and 400 mg/ml, respectively, and for other bacteria (Staphylococcus aureus, Bacillus cereus and Escherichia coli) it was more than 400 mg/ml.

کلیدواژه‌ها English

Essential oil
Lavandula stricta
Antimicrobial activity
Functional groups
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