بررسی اثر ضدقارچی اسانس مورت (Myrtus communis)بر پنی‌سیلیوم دیجیتاتوم و پنی‌سیلیوم ایتالیکوم (کپک‌های سبز و آبی میوه پرتقال)

نویسندگان
1 1- استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده
قارچ کش­های شیمیایی زیادی برای کنترل عفونت­های قارچی استفاده می­شوند. با این وجود، تعدادی از این قارچ کش­ها برای سلامتی انسان مضر هستند. امروزه، استفاده از مواد طبیعی مانند اسانس­های روغنی و عصاره­های گیاهی با کمترین عوارض جانبی مورد توجه پژوهشگران قرار گرفته است. مورت سبز (Hyssopus officinalis L)، متعلق به خانواده Lamiaceae است که به طور وسیعی در ایران رشد می­کند. هدف از این مطالعه، بررسی فعالیت اسانس مورت سبز برای کنترل سویه­های قارچی پنی­سیلیوم دیجیتاتوم و پنی­سیلیوم ایتالیکوم در شرایط آزمایشگاهی بود. ارزیابی فعالیت ضدقارچی اسانس مورت سبز به روش­های کربی-بوئر، چاهک آگار، رقت لوله­ای (ماکرودایلوشن براث) و حداقل غلظت کشندگی بررسی شد. نتایج نشان داد که قطر هاله عدم رشد (روش کربی-بوئر) برای سویه­های قارچی پنی­سیلیوم دیجیتاتوم و پنی­سیلیوم ایتالیکوم به ترتیب 60/10 و 70/9 میلی­متر بود. قطر هاله عدم رشد (روش چاهک آگار) برای سویه­های قارچی پنی­سیلیوم دیجیتاتوم و پنی­سیلیوم ایتالیکوم به ترتیب 30/12 و 50/10 میلی­متر بود. حداقل غلظت مهارکنندگی برای سویه­های قارچی پنی­سیلیوم دیجیتاتوم و پنی­سیلیوم ایتالیکوم به ترتیب 64 و 128 میلی­گرم بر میلی­لیتر بود. حداقل غلظت کشندگی اسانس مورت سبز نیز به ترتیب 512 و 512 میلی­گرم بر میلی­لیتر بود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigation of the antifungal effect of Myrtus communis essential oil on Penicillium digitatum and Penicillium italicum (the green and blue fungi of orange)

نویسندگان English

Mostafa Rahmati-Joneidabad 1
Behrooz Alizadeh behbahani 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

Much chemical fungicides are used to control fungal infections. However, several of these fungicides are detrimental for human health. Today, the use of natural ingredients such as essential oils and extracts plant with the least side effects has been of great interest to researchers. Myrtle (Myrtus communis L.) is a belongs to Myrtaceae family and a herb widely growing in Iran. The aim of this study was to examine the Myrtle essential oil to control the fungi (Penicillium digitatum and Penicillium italicum) “in vitro”. Evaluation of the antifungal activity of Myrtus communis essential oil was investigated by Kirby-Bauer, well agar, macro broth dilution and minimum fungicidal concentration methods. The results showed that the inhibition zone diameter (Kirby-Bauer method) for the fungal strains of Penicillium digitatum and Penicillium italicum was 10.60 and 9.70 mm, respectively. The results showed that the inhibition zone diameter (well agar method) for the fungal strains of Penicillium digitatum and Penicillium italicum was 12.30 and 10.50 mm, respectively. The minimum inhibitory concentration for Penicillium digitatum and Penicillium italicum was 64 and 128 mg/mL, respectively. The minimum fungicidal concentration was 512 and 512 mg/mL, respectively.

کلیدواژه‌ها English

Myrtus communis
Penicillium digitatum
Penicillium italicum
Antifungal activity
[1] Sinclair, W. B. (1984). Biochemistry and physiology of the lemon and other citrus fruits: University of California, Division of Agriculture and Natural Resources. pp: 946.
[2] Aboutalebi Jahromi, A. (2005). Study on the effect of heat treatment on controlling decay of citrus fruits. National symposium on losser of agricultural product‬. [Full text in Persian].
[3] Pourmir, S.Y., Sadeghi Mahoonak, A.R., Fattahi Moghaddam, J., Maghsoudlo, Y., Ghorbani, M. (2015). Physical characteristics, quantitative-qualitive properties and anti-oxidant materials of local orange (Citrus sinensis cv. Siavaraz) at harvesting times, during processing and storage. Iranian Food Science and Technology Research Journal, 11(5), 665-676. [Full text in Persian].
[4] Ayazpour, K., Hasanzadeh, H., Arabzadegan, M. S., & African, S. (2010). Evaluation of the control of citrus nematode (Tylenchulus semipenetrans) by leaf extracts of many plants and their effects on plant growth. African journal of agricultural research, 5(14), 1876-1880.
[5] Ghaemmaghami, S. S., Balali, G. R. (2013). Using pectic zymogram technique to identify interspecies variation of some Penicillium species. Journal of Molecular and Cellular Research, 26(4), 524-531. [Full text in Persian].
[6] Hadian, S., Hasanzadeh, N., aghajani, M., Pordeli, h., & Cirvillari, G. (2019). Evaluation and comparison of the antifungal effect of essential oil of four medicinal plants and molecular identification antagonistic bacteria against fungi decay cause of orange post-harvest by Penicillium digitatum. Eco-phytochemical Journal of Medicinal Plants, 7(3), 64-76.
[7] Swamy, M. K., & Sinniah, U. R. (2015). A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.: an aromatic medicinal plant of industrial importance. Molecules, 20(5), 8521-8547.
[8] Yadegarinia, D., Gachkar, L., Rezaei, M. B., Taghizadeh, M., Astaneh, S. A., & Rasooli, I. (2006). Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry, 67(12), 1249-1255.
[9] Cherrat, L., Espina, L., Bakkali, M., García‐Gonzalo, D., Pagán, R., & Laglaoui, A. (2014). Chemical composition and antioxidant properties of Laurus nobilis L. and Myrtus communis L. essential oils from Morocco and evaluation of their antimicrobial activity acting alone or in combined processes for food preservation. Journal of the Science of Food and Agriculture, 94(6), 1197-1204.
[10] Alizadeh Behbahani, B., Yazdi, F. T., Noorbakhsh, H., Riazi, F., Jajarmi, A., & Yazdi, F. T. (2016). Study of the antibacterial activity of methanolic and aqueous extracts of Myrtus communis on pathogenic strains causing infection. Zahedan Journal of Research in Medical Sciences, 18(2), 1-7.
[11] Alizadeh Behbahani, B., & Imani Fooladi, A. A. (2018). Development of a novel edible coating made by Balangu seed mucilage and Feverfew essential oil and investigation of its effect on the shelf life of beef slices during refrigerated storage through intelligent modeling. Journal of Food Safety, 38(3), e12443.
[12] Alizadeh behbahani, B., Tabatabaei Yazdi, F., Shahidi, F., & Mohebbi, M. (2014). Antifungal effect of aqueous and methanolic Avicennia marina leaves extracts on Alternaria alternata and Penicillium citrinum. Journal of Rafsanjan University of Medical Sciences, 12(12), 1015-1024.
[13] Barzegar, H., Mehrnia, M.A., Alizadeh Behbahani, B. (2018). Determination of the chemical composition, antioxidant activity and the antimicrobial effect of Heracleum Lasiopetalum on infection and food poisoning microorganisms. Journal of Applied Microbiology in Food Industry, 4(4), 15-28. [Full text in Persian].
[14] Noshad, M., Hojjati, M., & Alizadeh Behbahani, B. (2018). Black Zira essential oil: Chemical compositions and antimicrobial activity against the growth of some pathogenic strain causing infection. Microbial Pathogenesis, 116, 153-157.
[15] Alizadeh behbahani, B., Noshad, M., Falah, F. (2019). Investigation of antimicrobial activity of Fennel essential oil on some pathogenic microorganisms causing infection and food poisoning and its interaction with kanamycin antibiotic. Food Science and Technology, 16 (91), 233-241. [Full text in Persian].
[16] Amensour, M., Sendra, E., Abrini, J., Bouhdid, S., Pérez-Alvarez, J. A., & Fernández-López, J. (2009). Total phenolic content and antioxidant activity of myrtle (Myrtus communis) extracts. Natural product communications, 4(6), 819-924.
[17] Farzaneh, M., Kiani, H., Sharifi, R., Reisi, M., & Hadian, J. (2015). Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest Biology and Technology, 109, 145-151.
[18] Alizadeh Behbahani, B., Falah, F., Lavi Arab, F., Vasiee, M., & Tabatabaee Yazdi, F. (2020). Chemical composition and antioxidant, antimicrobial, and antiproliferative activities of Cinnamomum zeylanicum bark essential oil. Evidence-Based Complementary and Alternative Medicine, 2020, 1-8.
[19] Alizadeh Behbahani, B., & Shahidi, F. (2019). Melissa officinalis essential oil: Chemical compositions, antioxidant potential, total phenolic content and antimicrobial activity. Nutrition and Food Sciences Research, 6(1), 17-25.
[20] Alizadeh Behbahani, B., Shahidi, F., Yazdi, F. T., Mortazavi, S. A., & Mohebbi, M. (2017). Antioxidant activity and antimicrobial effect of tarragon (Artemisia dracunculus) extract and chemical composition of its essential oil. Journal of Food Measurement and Characterization, 11(2), 847-863.
[21] Golshan Tafti, A., & Baradaran, G. (2019). Study of the effect of essential oils in controlling of blue and green molds rot in orange fruit. Journal of Food Microbiology, 6(3), 12-21. [Full text in Persian].
[22] Shirzadi, H., & Abotalebi Jahromi, A. (2018). Inhibitory effect of medicinal plant essential oils on the growth of Penicillium sp. isolated from sweet orange. Journal of Microbial World, 11(3), 288-293. [Full text in Persian].
[23] Rasooli, I., Moosavi, M., Rezaee, M., Jaimand, K. (2010). Susceptibility of microorganisms to Myrtus communis L. essential oil and its chemical composition. Journal of Agricultural Science and Technology, 4,127–33.