ارزیابی فعالیت ضدقارچی اسانس اناریجه بر آسپرژیلوس نایجر (کپک سیاه) و بوتریتیس سینه‌را (کپک خاکستری) عامل فساد میوه انگور: مطالعه در شرایط آزمایشگاهی

نویسندگان
مصطفی رحمتی جنیدآباد 1
بهروز علیزاده بهبهانی 2
1 استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.52547/fsct.17.108.75
چکیده
در حال حاضر افزایش آگاهی مردم نسبت به سلامت و خواص تغذیه­ای محصولات کشاورزی و فراورده­های آن­ها، و از سویی دیگر عوارض ناشی از سموم شیمیایی و نگهدارنده­های سنتزی تقاضا برای مصرف مواد غذایی تازه و عاری از مواد نگهدارنده شیمیایی و سنتزی را دوچندان نموده است. اناریجه گیاهی از خانواده چتریان است و بومی بخش­هایی از مناطق مرکزی و شمالی ایران می­باشد. برای ارزیابی فعالیت ضدقارچی اسانس اناریجه از روش­های دیسک دیفیوژن آگار، چاهک آگار، حداقل غلظت مهارکنندگی (ماکرودایلوشن براث و رقت آگار) و حداقل غلظت کشندگی استفاده شد. نتایج نشان داد که اسانس اناریجه به خوبی توانست از رشد سویه­های قارچی عامل فساد سیاه و خاکستری انگور در شرایط آزمایشگاهی جلوگیری کند. نتایج نشان داد قطر هاله بازدارندگی در روش دیسک دیفیوژن آگار برای سویه­های قارچی آسپرژیلوس نایجر و بوتریتیس سینه­را به ترتیب 50/15 و 30/13 میلی­متر بود. نتایج اثر ضدقارچی در روش چاهک آگار به مراتب هاله بازدارندگی بزرگتری نسبت به روش دیسک دیفیوژن از خود نشان داد. حداقل غلظت کشندگی برای سویه­های قارچی آسپرژیلوس نایجر و بوتریتیس سینه­را به ترتیب 64 و 256 میلی­گرم بر میلی­لیتر بود. حداقل غلظت کشندگی اسانس اناریجه برای هر دو سویه آسپرژیلوس نایجر و بوتریتیس سینه­را نسبت به حداقل غلظت مهارکنندگی بیشتر بود.
کلیدواژه‌ها
اناریجه
آسپرژیلوس نایجر
بوتریتیس سینه‌را
قطر هاله بازدارندگی

موضوعات

عنوان مقاله English

Evaluation of the antifungal effect of Froriepia subpinnata essential oil on Aspergillus niger (black mold) and Botrytis cinerea (gray mold) grape poisoning agent: A study "in vitro"

نویسندگان English

Mostafa Rahmati-Joneidabad 1
Behrooz Alizadeh behbahani 2
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
چکیده English

At present, increasing public awareness of the health and nutritional properties of agricultural products and their products, as well as the effects of chemical toxins and synthetic preservatives, doubles the demand for fresh and chemical-free food preservatives. Froriepia subpinnata is a member of the family Umbelliferae. This plant is native central and northern parts of Iran. Disk diffusion agar, well diffusion agar, minimum inhibitory concentration (broth microdilution and agar dilution) and minimum fungicidal concentration were used to evaluated the antifungal effect of Froriepia subpinnata essential oil. The results showed that Froriepia subpinnata essential oil was well able to prevent the growth of fungal strains that cause black and gray spoilage in vitro. The results showed that the inhibition zone diameter (disc diffusion method) for the fungal strains of Aspergillus niger and Botrytis cinerea was 15.50 and 13.30 mm, respectively. The results of the antifungal effect in the well agar method showed a much greater inhibition zone diameter than the disc diffusion method. The minimum fungicidal concentration for Aspergillus niger and Botrytis cinerea was 64 and 256 mg/mL, respectively. The minimum fungicidal concentration of Froriepia subpinnata essential oil was higher for Aspergillus niger and Botrytis cinerea strains than the minimum inhibitory concentration.

کلیدواژه‌ها English

Froriepia subpinnata
Aspergillus niger
Botrytis cinerea
Inhibition zone diameter
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