تولید گاما آمینو بوتریک اسید توسط باکتری Lactococcus lactis NZ1330 در محیط کشت حاوی لجن لبنی و مونوسدیم گلوتامات

نویسندگان
بهروز علیزاده بهبهانی 1
فرشته فلاح 2
علیرضا وسیعی 2
farideh tabatabaei yazdi 3
سیدعلی مرتضوی 3
1 استادیارگروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 دانشجوی دکتری گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
3 استاد گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
10.29252/fsct.16.97.89
چکیده
گاما آمینو­بوتریک­اسید (گابا)، مولکول زیست فعال با نقش­های فیزیولوژیکی مختلف در بدن است که با مهار تحریکات نورون و ممانعت از رسیدن پیام های حاوی ترکیبات استرس­زا، دارای خواص آرامش بخشی بوده و در درمان بیماری­های مختلف نقش موثری دارد. در پژوهش حاضر، امکان تولید این اسید آمینه توسط باکتری Lactococcus lactis NZ1330 بررسی شد. به منظور بهینه­سازی فرآیند تخمیر سه سطح از لجن لبنی(5،10،15 درصد)، مونو سدیم گلوتامات (0، 5/0 و 1 درصد) در زمان­های 24، 48 و 72 ساعت انتخاب شد و پس از تخمیر، وجود گابا در محیط کشت به وسیله کروماتوگرافی لایه نازک بررسی شد. برای کمی سازی باندهای موجود در کروماتوگرافی لایه نازک از روش اسپکتروفتومتری استفاده شد. نتایج بهینه­سازی در سطح معنی داری 95 درصد نشان داد تیمار بهینه شامل محیط کشت حاوی 2/11درصد لجن لبنی،7/0 درصد مونوسدیم گلوتامات و زمان 70 ساعت تخمیر در دمای °C 32 بوده و تحت این شرایط تولید گابا به میزان ppm 400 می­باشد. بنابراین از این ترکیب محیط کشت می­توان به عنوان سوبسترای مناسب جهت تولید ترکیب دارویی و زیست فعال ارزشمند گابا استفاده کرد.
کلیدواژه‌ها
گابا
لاکتوکوکوس
لجن سپراتور
مونوسدیم گلوتامات

موضوعات

عنوان مقاله English

Gamma-aminobutyric acid Synthesis by Lactococcus lactis strain Nz1330 in Dairy sludge medium with Monosodium glutamate

نویسندگان English

Behrooz Alizadeh behbahani 1
Fereshteh Falah 2
Alireza Vasiee 2
farideh tabatabaei yazdi 3
Seyed Ali Mortazavi 3
1 Assistant professor, Department of Food Science and Technology, Faculty of Animal Science and Food
2 Ph.D. Student, Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Professor, Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده English

Gamma Aminobutyric Acid (GABA) is a bioactive molecule with different physiological roles in the body that inhibits neuronal stimulation and inhibits the delivery of stress-containing messages, has a calming effect and is used to treat diseases. Different has an effective role. In the present study, the possibility of producing this amino acid by Lactococcus lactis NZ1330 was investigated. In order to optimize the fermentation process three levels of dairy sludge (5,10,15%), monosodium glutamate (0, 0.5 and 1%) were selected at 24, 48 and 72 hours after fermentation. The presence of GABA in the culture medium was investigated by thin layer chromatography. Spectrophotometric method was used to quantify the bands present in thin-layer chromatography. Optimization results at 95% significance level showed that the optimum treatment consisted of medium containing 11.2% dairy sludge, 0.7% monosodium glutamate and 70 hours fermentation at 32 ° C and under these conditions, GABA production was ppm. It's 400. Therefore, this combination of media can be used as a suitable substrate for the production of valuable GABA drug and bioactive compounds

کلیدواژه‌ها English

GABA
Lactococcus
Dairy sludge
Monosodium glutamate
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