ارزیابی فعالیت ضدمیکروبی اسانس پونه بر تعدادی از پاتوژن‌های غذایی و برهمکنش آن با آنتی‌بیوتیک‌های جنتامایسین و کلرامفنیکل در شرایط محیط کشت

نویسندگان
هادی تناور 1
حسن برزگر 2
بهروز علیزاده بهبهانی 3
محمدامین مهرنیا 3
1 دانشجوی کارشناسی ارشد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
2 گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
3 استادیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
10.29252/fsct.16.97.77
چکیده
در طب سنتی از گیاه پونه جهت درمان سینوزیت، ناراحتی­های دستگاه گوارش، اختلالات سیستم تنفسی و رفع مسمومیت­های غذایی استفاده می­شود. در این پژوهش فعالیت ضدمیکروبی اسانس پونه بر تعدادی از پاتوژن­های غذایی مورد بررسی قرار گرفت. جهت بررسی برهمکنش اسانس پونه با آنتی­بیوتیک­ جنتامایسین و کلرامفنیکل از غلظت­های تحت مهاری استفاده شد. نتایج نشان داد که اسانس پونه توانایی مهار رشد میکروارگانیسم­های پاتوژن را بر سطح محیط کشت داشت. قطر هاله عدم رشد (هاله بازدارندگی) به روش انتشار در آگار با استفاده از دیسک برای باکتری­های لیستریا اینوکوا، استافیلوکوکوس اورئوس، اشرشیا کلی، سالمونلا تیفی و سودوموناس ائروژینوزا به ترتیب 50/16، 60/14، 10، 14 و 10 میلی­متر بود. میانگین قطر هاله بازدارندگی به روش انتشار در آگار با استفاده از چاهک برای باکتری­های لیستریا اینوکوا، استافیلوکوکوس اورئوس، اشرشیا کلی، سالمونلا تیفی و سودوموناس ائروژینوزا به ترتیب 40/14، 16، 12، 30/14 و 10/10 میلی­متر بود. نتایج نشان داد که در حالت ترکیب اسانس پونه با آنتی­بیوتیک جنتامایسین برای تمامی باکتری­ها حالت سینرژیستی مشاهده شد. در حالت ترکیبی اسانس پونه با آنتی­بیوتیک کلرامفنیکل برای باکتری­های استافیلوکوکوس اورئوس، اشرشیا کلی و سودوموناس ائروژینوزا حالت سینرژیستی مشاهده گردید. حداقل غلظت مهارکنندگی اسانس پونه برای تمامی باکتری­های 25/6 میلی­گرم بر میلی­لیتر بود. به طور کلی با توجه به نتایج به دست آمده می­توان از اسانس پونه جهت کنترل رشد میکروارگانیسم­های بیماری­زا در مواد غذایی استفاده کرد.
کلیدواژه‌ها
اسانس پونه
اثر سینرژیستی
پاتوژن‌های غذایی
برهمکنش

موضوعات

عنوان مقاله English

Evaluation of the antimicrobial activity of Mentha pulegium essential oil on some foodborne pathogens and its interaction with gentamicin and chloramphenicol in vitro

نویسندگان English

Hadi Tanavar 1
Hassan Barzegar 2
Behrooz Alizadeh behbahani 3
Mohammad Amin Mehrnia 3
1 M. Sc Student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
2 Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
3 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

In traditional medicine, Mentha pulegium was used for sinusitis treatment, gastrointestinal disorders, respiratory disorders and food detoxity. In this research, Mentha pulegium essential oil (MPEO) antibacterial activity on some foodborne pathogens was considered. For research on MPEO interaction with chloramphenicol and gentamicin antibiotic, sub-minimum inhibitory concentration was used. The results showed that in growth medium MPEO able to control pathogen microorganisms. The inhibition zone diameter (IZD), in disk diffusion method for Listeria innocua, Staphylococus aureus, Escherchia coli, Salmonella typhi, Pseudomonas aeruginosa bacteria was 16/50, 14/60, 10, 14, 10 mm respectively. The mean of IZD in well diffusion method for Listeria innocua, Staphylococus aureus, Escherchia coli, Salmonella typhi, Pseudomonas aeruginosa bacteria was 14/40, 16, 12, 14/30, 10, 10/10 mm respectively. The results showed that in combination of MPEO with gentamicin antibiotic, for all bacteria, synergistic was observed. In combination of MPEO, with chloramphenicol antibiotic in Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, synergistic state was observed. Minimum inhibitory concentration in MPEO for all bacteria was 6/25 mg/ml. In general, with regard to acquired results, one can use MPEO for pathogenic microorganisms growth control in foods.

کلیدواژه‌ها English

Mentha pulegium essential oil
Synergistic
Foodborne pathogens
Interaction
[1] Gholami Pornaki, P., Aghazadeh, M., & Sadeghi, M.R. 2017. Evaluation of chemical composition and in-vitro antibacterial activity of oregano (Mentha pulegium) growing wild in maku and its inhibitory effect on Staphylococcus aureus in sausage. Veterinary Researches & Biological Products. 117, 69-77. [full text in Persian]
[2] Pajohi, M.R., Tajik, H., Akhondzade, A., Gandomi, H., Ehsani, A., Shokohi Sabet Jalali, F. 2010. Evaluation of chemical composition and antimicrobial efficacy of Cuminum cyminum L. and Mentha longifolia L. alone and combined with nisin. Urmia Medical Journal. 21, 324-331. [full text in Persian]
[3] Mahmodi, R., Tajik, H., Farshid, A.A., Ehsani, A, Zaree, P, & Moradi, M. 2011. Phytochemical properties of Mentha longifolia L. essential oil and its antimicrobial effects on Staphylococcus aureus. Medical Sciences Journal. 16(5), 400-412. [full text in Persian]
[4] Azizi Tabrizzad, N., Seyedin Ardebili, S. M., & Hojjati, M. 2019. Investigation of chemical compounds and antibacterial activity of pennyroyal, mint and thyme essential oils. Journal of Fisheries Science and Technology.15(85), 447-457. [full text in Persian]
[5] Teimoori, P., Yahyaabady, S., Doudi, M. 2018. Antimicrobial effect of Mentha Longifulia on different bacteria causing diarrhea and its phytochemical evaluation. New Cellularand Molecular Biotechnology Journal. 8, 65-72. [full text in Persian]
[6] Rahmani, F., Rezaeian-Doloei, R., & Ali Moradi, L. 2018. Evaluation of phytochemical composition of Mentha pulegium L. essential oil and its antibacterial activity against several pathogenic bacteria .Iranian Journal of Medical Microbiology. 11(6), 167-177. [full text in Persian]
[7] Farahmandfar, R., & Kordjazi, A. 2019. Antimicrobial effect of cardin (Biarum bovei) extract against
Escherichia coli and Staphylococcus aureus: studies in vitro and hamburger. Journal of Fisheries Science and Technology.16(86), 1-13. [full text in Persian]
[8] Mohammadpour Kanzaq, H., Noroozi, M., Mahmoudi, R., Mohammadpoorasl, A., Zavoshy, R., & AsadiNadari, M. 2015. Antimicrobial effect of stachys lavandulifolia yachl essential oil on Listeria Monocytogenes. Medical Laboratory Journal. 9(2), 47-53. [full text in Persian]
[9] Valadbeigi, T., & Chalabzardi, M. 2015. Antimicrobial effect of scrophularia striata extract on
Escherichia coli strains isolated from patients with urinary tract infection in ilam. Scientific Journal of Ilam University of Medical Sciences. 24(2), 158-166. [full text in Persian]
[10] Zeinali, T., Mohsenzadeh, M., RezaeianDoloei, R., & Nabipour, R. 2016. In vitro assessment of antimicrobial effect of methanolic extract of peganum harmala against some important foodborne pathogens. bacterial. Journal of Food Hygiene. 5(4), 27-36. [full text in Persian]
[11] Ghotaslou, R., Saghati, H., Dehnad, A., Salahi Eshlaghi, B. 2016. Antibacterial effects of azerbaijan honey on Pseudomonas aeruginosa biofilm. Iranian Journal of Medical Microbiology. 9(4), 40-46. [full text in Persian]
[12] Behdani, M., Ghazvini, K., Mohammadzadeh, A.R., & Sadeghian, A. 2009. Antibacterial activity of henna extracts against Staphylococcus aureus and Pseudomonas aeruginosa. GMUHS Journal. 15(3), 46-52. [full text in Persian]
[13]. Jalali Nadoushan, M.R., Ghosian Moghadam, M.H., jafari, H., & Fallah, N. 2008. The effect of aqueous garlic extract on clinical manifestations in Salmonella typhimorium infected rabbits. Iranian Journal of Medicinal and Aromatic Plants. 24(1), 74-81. [full text in Persian]
[14] Ghadimipour, R., Sedigh-Eteghad, S., Chalangar, R., Alipoor-yeganeh, M., khadiri, B., & Afkari, G.h. 2015. In Vitro antibacterial properties of aqueous extract of garlic against on common diarrhea causing bacteria. Journal of North Khorasan University. 7(1), 357-367. [full text in Persian]
[15] Tabatabaei Yazdi, F., Alizade Behbahani, B., & Heidari Sureshjani, M. 2014. The comparison of antimicrobial effects of Chevil (Ferulago angulata) extract with a variety of common therapeutic antibiotics in vitro. Journal of Arak University of Medical Sciences. 17(3), 35-46.
[16] Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Fakhri, S., Mortazavi, S. A., & Mohebbi, M. 2017. Investigation of chemical compounds and antibacterial activity of Tarragon (Artemisia dracunculus) essential oil on some pathogenic bacteria in vitro. Qom University of Medical Sciences Journal. 11(9), 42-51. [full text in Persian]
[17] Bauer, A., Kirby, W., Sherris, J.C., & Turck, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 45(4), 493-496.
[18] Hamonnavard, S., Bahrami, AM., Razmjou, M., Asadisamani, M., & Hatamilak, M. 2013. Evaluation of nerium oleander aqueous extract effect on Staphylococcus aureus and Staphylococcus epidermis. Journal of Shahrekord University of Medical Sciences. 15, 46-54. [full text in Persian]
[19] Alizadeh Behbahani B, Shahidi F, Tabatabaei Yazdi F, Mortazavi S.A, & Mohebbi, M. 2017. Antioxidant activity and antimicrobial effect of tarragon (Artemisia dracunculus) extract and chemical composition of its essential oil. Journal of Food Measurement and Characterization. 11, 847-863.
[20] Alizadeh Behbahani, B., & Fooladi, AAI. 2018. Evaluation of phytochemical analysis and antimicrobial activities allium essential oil against the growth of some microbial pathogens. Microbial Pathogensis. 114, 299-303.
[21] Daneshmandi, S., Soleimani, N., Pourfathollah, A.A., & Sattari, M. 2010. Evaluation of the drug synergistic and antibacterial effects of cuminum cyminum essential oil. Journal of Arak University of Medical Sciences. 13(2), 75-82.
[22] Kermanshah, H., Hashemi Kamangar, S. S., Arami, S., Mirsalehian, A., Kamalinejad, M., Karimi, M. 2011. In vitro evaluation of antibacterial activity of hydroalcoholic extract of Salvia officinalis and Menta longifolia against three cariogenic bacteria. Journal of Dental School Shahid Beheshti University of Medical Sciences. 28, 232-237. [full text in Persian]
[23] Ataie Kachouei, M. 2016. Study the antimicrobial effects of the essential oils of Origanum vulgare, Mentha piperita and Carum carvi on the bacteria isolates from food stuffs. Journal of Food Microbiology. 3(1), 1-10. [full text in Persian]
[24] Nozohour, Y., Rasoulifard, M., & Ghahramanigermi, N. 2016. Evaluation of antibacterial properties of oregano essence on pathogenic bacteria isolated from hospital infections. Scientific Journal of Ilam University of Medical Sciences. 25(5), 154-160. [full text in Persian]
[25] Ghorbani, M., Ahmady-Asbchin, S., & Rezaei, H. 2018. Evaluation of antibacterial properties of Coriander, Oregano, Fennel, Thyme and parsley extracts, on pathogenic bacteria Staphylococcus aureus (ATCC 33591), Escherichia coli (ATCC 23591), Klebsiella (ATCC 10031) and Salmonella Typhimurium. Journal of Sabzevar University of Medical Sciences. 25(4), 591-598. [full text in Persian]
[26] Misaghi, A., Saeedi, M., Noori, N., & Rezaeigolestani, M.R. 2018. Study of effect of oregano essential oil and ethanolic extract of propolis on antibacterial properties and some physical characteristics of biodegradable poly-lactic acid films. Iranian Journal of Health and Environment. 11(1), 111-122. [full text in Persian]
[27] Alizadeh Behbahani, B., Noshad, M., & Falah, F. 2019. Cumin essential oil: Phytochemical analysis, antimicrobial activity and investigation of its mechanism of action through scanning electron microscopy. Microbial Pathogenesis. 136, 1-5.
[28] Ghani, S., Barzegar, H., Noshad, M., & Hojjati, M. 2018. The preparation, characterization and in vitro application evaluation of soluble soybean polysaccharide films incorporated with cinnamon essential oil nanoemulsions. International Journal of Biological Macromolecules. 112, 197-202.
[29] Barzegar, H., Alizadeh, V. Characterization of physical, antioxidant and antimicrobial properties of biodegradable soluble soybean polysaccharide films containing Satureja hortensis essential oil. Food Science and Technology. 14(71), 35-45. [full text in Persian]
[30] Kouravand, F., Jooyandeh, H., Barzegar, H., & Hojjati, M. 2018. Characterization of cross‐linked whey protein isolate‐based films containing Satureja Khuzistanica Jamzad essential oil. Journal of Food Processing and Preservation. 42(3). 1-10.
[31] Alizadeh, V., Barzegar, H., Nasehi, B., Samavati, V. Characterization of physical and antimicrobial properties of chitosan edible films containing Pistacia atlantica gum essence. Iranian Food Science and Technology Research Journal. 13(4), 584-593. [full text in Persian]
[32] Cherrat, L., Espina, L., Bakkali, M., Pag´an, R., & Laglaoui, A. 2013. Chemical composition, antioxidant and antimicrobial properties of Mentha pulegium, lavandula stoechas and satureja calamintha scheele essential oils and an evaluation of their bactericidal effect in combined processes. Innovative Food Science and Emerging Technologies. 22, 221-229.
[33] Sbayou, H., Ababou, B., Boukachabine, K., Manresa, A., Zerouali,K., & Amghar, S. 2014. Chemical composition and antibacterial activity of artemisia herba-alba and Mentha pulegium essential oils. Journal of Life Sciences. 8(1), 35-41.
[34] Chraibi, M., Farah, A., Lebrazi, S., Elamin, O., Iraqui Houssaini, M., & Fikri-Benbrahim, K. 2016. Antimycobacterial natural products from moroccan medicinal plants: chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils. Asian Pacific Journal of Tropical Biomedicine. 6(10), 836-840.
[35] Khosravi Zanjani, M.A., Mohammadi, N., Zojaji, M., & Bakhoda, H. 2015. Chemical composition of the essential oil of Mentha pulegium L. and its antimicrobial activity on proteus mirabilis, Bacillus subtilis and Zygosaccharomyces rouxii. Journal of Food Biosciences and Technology.5(2), 31-40.
[36] Bouyahya, A., Et-Touys, A., Bakri, Y., Talbaui, A., Fellah, H., Abrini, J., & Dakka, N. 2017. Chemical composition of Mentha pulegium and rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities. Microbial Pathogenesis. 111, 41-49.