Assessing Ozonated Water's Ability to Reduce Malondialdehyde in Red Meat Samples from Iraq's Wasit Markets

نویسنده
گروه پرستاری / موسسه فنی سواره، دانشگاه فنی میانه، بغداد، عراق
چکیده
Malondialdehyde (MDA), which is a secondary result of oxidation, is the major well-studied detrimental by-product of the peroxidation of polyunsaturated fatty acids. It has been used as a measurement of oxidative rancidity. The sensitive thiobarbituric acid reactive substance (TBARS) test makes it possible to determine the amount of MDA present in animal tissues. This study aimed to assess ozonated water's ability to reduce MDA in red meat samples retailed in Iraq's Wasit markets. There was a determination of MDA concentrations using the use of high-performance liquid chromatography (HPLC). An ozone generator (A2Z/AQUA-6, USA) was used to create ozone (O3), and a CHE-Mets®-Kit (USA) was used to determine the concentration of O3 in water in terms of parts per million (ppm). Before treatment (control), the findings showed that all of the samples had higher MDA concentrations, with levels in frozen sheep meat (1.53-2.51 ppm) and cow meat (0.89-1.71) ppm. After being treated with ozonated water (0.5 ppm for thirty minutes), all of the samples exhibited a decrease in the measured levels of MDA (ppm). It was observed that the average levels of MDA in frozen sheep and cow meat were 1.27± 0.28 and 1.99± 0.32, respectively, before the application of treatment. However, after treatment, the average levels of MDA were found to be 1.13±0.323 for frozen sheep meat and 0.39±0.20 for frozen cow meat. There was a statistically significant difference between these two groups (P≤ 0.05). This decrease in MDA is very important from the point of view of public health
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Assessing Ozonated Water's Ability to Reduce Malondialdehyde in Red Meat Samples from Iraq's Wasit Markets

نویسنده English

Manal Hadi Ghaffoori Kanaan
Department of Nursing / Technical Institute of Suwaria, Middle Technical University, Baghdad, Iraq
چکیده English

Malondialdehyde (MDA), which is a secondary result of oxidation, is the major well-studied detrimental by-product of the peroxidation of polyunsaturated fatty acids. It has been used as a measurement of oxidative rancidity. The sensitive thiobarbituric acid reactive substance (TBARS) test makes it possible to determine the amount of MDA present in animal tissues. This study aimed to assess ozonated water's ability to reduce MDA in red meat samples retailed in Iraq's Wasit markets. There was a determination of MDA concentrations using the use of high-performance liquid chromatography (HPLC). An ozone generator (A2Z/AQUA-6, USA) was used to create ozone (O3), and a CHE-Mets®-Kit (USA) was used to determine the concentration of O3 in water in terms of parts per million (ppm). Before treatment (control), the findings showed that all of the samples had higher MDA concentrations, with levels in frozen sheep meat (1.53-2.51 ppm) and cow meat (0.89-1.71) ppm. After being treated with ozonated water (0.5 ppm for thirty minutes), all of the samples exhibited a decrease in the measured levels of MDA (ppm). It was observed that the average levels of MDA in frozen sheep and cow meat were 1.27± 0.28 and 1.99± 0.32, respectively, before the application of treatment. However, after treatment, the average levels of MDA were found to be 1.13±0.323 for frozen sheep meat and 0.39±0.20 for frozen cow meat. There was a statistically significant difference between these two groups (P≤ 0.05). This decrease in MDA is very important from the point of view of public health

کلیدواژه‌ها English

: ozonated water
sheep meat
MDA
Al- Suwaria markets
1. Ruban SW. Lipid peroxidation in muscle foods-an overview. 2009: 509-513.
2. Kanaan MH, Mohammed FA, Abdullah SS. The Effectiveness of Aqueous Ozone on Residual Chlorination by Products in Treated Chicken Meat. InIOP Conference Series: Earth and Environmental Science 2024 Jul 1 (Vol. 1371, No. 6, p. 062036). IOP Publishing.
3. Khadre MA, Yousef AE, Kim JG. Microbiological aspects of ozone applications in food: a review. Journal of food science. 2001 Nov;66(9):1242-52.
4. Cárdenas FC, Andrés S, Giannuzzi L, Zaritzky N. Antimicrobial action and effects on beef quality attributes of a gaseous ozone treatment at refrigeration temperatures. Food Control. 2011 Aug 1;22(8):1442-7.
5. Muhlisin M, Utama DT, Lee JH, Choi JH, Lee SK. Effects of gaseous ozone exposure on bacterial counts and oxidative properties in chicken and duck breast meat. Korean Journal for Food Science of Animal Resources. 2016;36(3):405.
6. KIM JG, Yousef AE, Chism GW. Use of ozone to inactivate microorganisms on lettuce. Journal of Food Safety. 1999 Apr;19(1):17-34.
7. Mohammed FA, Kanaan MH, Tarek AM. Assessment of the effect of aqueous ozone treatment on the sensory attributes of retail meat in the Iraqi Wasit governorate. Revista Electronica de Veterinaria. 2022 May 17:28-38.
8. Kanaan MH, Khashan HT. Molecular typing, virulence traits and risk factors of pandrug-resistant Acinetobacter baumannii spread in intensive care unit centers of Baghdad city, Iraq. Reviews and Research in Medical Microbiology. 2022 Jan 1;33(1):51-5.
9. Kanaan MH, Al-Shadeedi SM, Al-Massody AJ, Ghasemian A. Drug resistance and virulence traits of Acinetobacter baumannii from Turkey and chicken raw meat. Comparative immunology, microbiology and infectious diseases. 2020 Jun 1;70:101451.
10. Kanaan MH. Prevalence, resistance to antimicrobials, and antibiotypes of Arcobacter species recovered from retail meat in Wasit marketplaces in Iraq. Int J One Health. 2021 7: 1 142- 150.
11. Kanaan MH, Anah SA, Jasim GA, Ghasemian A. In-vitro protoscolicidal and immunomodulatory effects of Cinnamomum camphora and Ziziphora tenuior against Echinococcus granulosus protoscolices. Reviews and Research in Medical Microbiology. 2021 Jan 1;32(1):45-50.
12. Kanaan MH, Tarek AM. Clostridium botulinum, a foodborne pathogen and its impact on public health. Ann. Trop. Med. Publ. Health. 2020;23(5):49-62.
13. Kanaan MH, Khalil ZK, Khashan HT, Ghasemian A. Occurrence of virulence factors and carbapenemase genes in Salmonella enterica serovar Enteritidis isolated from chicken meat and egg samples in Iraq. BMC microbiology. 2022 Nov 22;22(1):279.
14. Kanaan MH. Prevalence and antimicrobial resistance of Salmonella enterica serovars Enteritidis and Typhimurium isolated from retail chicken meat in Wasit markets, Iraq. Veterinary World. 2023 Mar;16(3):455.
15. Kanaan MH, Al-Isawi AJ. Prevalence of methicillin or multiple drug-resistant Staphylococcus aureus in cattle meat marketed in Wasit province. Biochemical & Cellular Archives. 2019 Apr 1;19(1).
16. Kanaan M, Abdullah S. Evaluation of aqueous Ozone as a method to combat multidrug-resistant Staphylococcus aureus tainting cattle meat sold in Wasit marketplaces. Mansoura Veterinary Medical Journal. 2021 Sep 30;22(3):117-23.
17. Kanaan MH, Tarek AM, Abdullah SS. Prevalence and antimicrobial resistance of Staphylococcus aureus in some types of dairy products in Baghdad Markets. J Dis Global Health. 2023 Jan 5;16(1):1-7.
18. Kanaan MH, Tarek AM. Innovative modern bio-preservation module of meat by lytic bacteriophages against emergent contaminants. Open Veterinary Journal. 2022;12(6):1018-26.
19. Hadi Ghaffoori Kanaan, M Jebur Obayes Al-Isawi A, Ahmad Mohamme F. Antimicrobial Resistance and Antibiogram of Thermotolerant Campylobacter Recovered from Poultry Meat in Baghdad Markets, Iraq. Arch Razi Inst. 2022; 77 (1): 231- 237.
20. Kanaan MH, Abdulwahid MT. Prevalence rate, antibiotic resistance and biotyping of thermotolerant Campylobacter isolated from poultry products vended in Wasit markets. Current Research in Nutrition and Food Science Journal. 2019 Dec 25;7(3):905-17.
21. Kanaan MH, Mohammed FA. Antimicrobial resistance of Campylobacter jejuni from poultry meat in local markets of Iraq. Plant Arch. 2020;20(1):410-5.
22. Kanaan MH, Salim ID, Tarek AM, Abdullah SS. Knowledge, attitude, and hygiene practices of food handlers related to food safety in Al-Suwaira City, Wasit Province in Iraq. International Journal of One Health. 2023;9(2):150-8.
23. Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Analytical biochemistry. 2017 May 1;524:13-30.
24. Bertolín JR, Joy M, Blanco M. Malondialdehyde determination in raw and processed meat products by UPLC-DAD and UPLC-FLD. Food chemistry. 2019 Nov 15;298:125009.
25. Cunha LC, Monteiro ML, Lorenzo JM, Munekata PE, Muchenje V, De Carvalho FA, Conte-Junior CA. Natural antioxidants in processing and storage stability of sheep and goat meat products. Food Research International. 2018 Sep 1;111:379-90.
26. Kronn TG. Nonthermal plasma treatment of packaged broiler breast fillets to reduce natural microflora and Campylobacter jejuni (Doctoral dissertation, University of Georgia). 2013.
27. Reitznerová A, Šuleková M, Nagy J, Marcinčák S, Semjon B, Čertík M, Klempová T. Lipid peroxidation process in meat and meat products: A comparison study of malondialdehyde determination between modified 2-thiobarbituric acid spectrophotometric method and reverse-phase high-performance liquid chromatography. Molecules. 2017 Nov 16;22(11):1988.
28. Kanner J. Dietary advanced lipid oxidation endproducts are risk factors to human health. Molecular nutrition & food research. 2007 Sep;51(9):1094-101.
29. Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free radical biology and medicine. 1990 Jan 1;9(6):515-40.
30. Paulinus ON, Tinuade O. A comparative study of malondialdehyde contents of some meat and fish samples processed by different methods. Journal of Pharmaceutical and Scientific Innovation. 2013;2(4):26-9.
31. Kanaan MH. Antibacterial effect of ozonated water against methicillin-resistant Staphylococcus aureus contaminating chicken meat in Wasit Province, Iraq. Veterinary world. 2018 Nov;11(10):1445.
32. Pilz J, Meineke I, Gleiter CH. Measurement of free and bound malondialdehyde in plasma by high-performance liquid chromatography as the 2, 4-dinitrophenylhydrazine derivative. Journal of Chromatography B: Biomedical Sciences and Applications. 2000 Jun 9;742(2):315-25.
33. Marcinčák S, Sokol J, Bystrický P, Popelka P, Turek P, Bhide M, Máté D. Determination of lipid oxidation level in broiler meat by liquid chromatography. Journal of AOAC International. 2004 Dec 1;87(5):1148-52.
34. MedCalc Software Ltd. https://www.medcalc.org/calc/ php Version 22 021 accessed March 23, 2024.
35. Pandey MC, Harilal PT, Radhakrishna K. Effect of processing conditions on physico-chemical and textural properties of shami kebab. International Food Research Journal. 2014;21(1):223.
36. Hejazy M, Khatibi SA, Shamsi Z. The effect of frying process on the level of malondialdehyde in different meat products. Journal of Nutrition and Food Security. 2020.
37. Trindade MA, Felício PE, Castillo CJ. Mechanically separated meat of broiler breeder and white layer spent hens. Scientia Agricola. 2004;61:234-9.38. Campo, M. M.; Nute, G. R.; Hughes, S. I.; Enser, M.; Wood, J. D.; and Richardson, R. I. (2006). Flavor perception of oxidation in beef. Meat Sci., 72: 303-311.
39. Okolie NP, Akioyamen MO, Okpoba N, Okonkwo C. Malondialdehyde levels of frozen fish, chicken and turkey on sale in Benin City markets. African Journal of Biotechnology. 2009;8(23).
40. Guo X, Wang N, Wei Y, Liu P, Deng X, Lei Y, Zhang J. The Effects of Malonaldehyde on Quality Characteristics and Protein Oxidation of Coregonus peled (Coregonuspeled) during Storage. Foods. 2023 Feb 7;12(4):716.
41. Wójciak KM, Karwowska M, Dolatowski ZJ. Fatty acid profile, color and lipid oxidation of organic fermented sausage during chilling storage as influenced by acid whey and probiotic strains addition. Scientia Agricola. 2015 Feb;72(2):124-31.
42. Jongberg S, Carlsen CU, Skibsted LH. Peptides as antioxidants and carbonyl quenchers in biological model systems. Free Radical Research. 2009 Jan 1;43(10):932-42.
43. Sheldon, B.W.; Ball , H.; and Jr., R. (1984a) . Effects ozonated processing water on the organoleptic, microbial, physical and chemical characteristics of poultry products. Industry Synopsis for the Southeast Poultry & Egg Association , 1 : 84-88.
44. Graham DM, Strasser J, Mannapperuma JD. Applications of ozonation and membrane treatment in poultry processing. California Energy Com. Final Report (400-02-023F). Petaluma, California. 2002:1-29.
45. Trindade MA, Kushida MM, Villanueva ND, dos Santos Pereira DU, de Oliveira CE. Comparison of ozone and chlorine in low concentrations as sanitizing agents of chicken carcasses in the water immersion chiller. Journal of food protection. 2012 Jun 1;75(6):1139-43.
46. Jung S, Nam KC, Jo C. Detection of malondialdehyde in processed meat products without interference from the ingredients. Food Chemistry. 2016 Oct 15;209:90-4.
47. Muhlisin, Cho Y, Choi JH, Hahn TW, Lee SK. Bacterial Counts and Oxidative Properties of Chicken Breast Inoculated with S almonella Typhimurium Exposed to Gaseous Ozone. Journal of Food Safety. 2015 Feb;35(1):137-44.
48. Gertzou IN, Drosos PE, Karabagias IK, Riganakos KA. Combined effect of ozonation and packaging on shelf life extension of fresh chicken legs during storage under refrigeration. Journal of food science and technology. 2016 Dec;53:4270-7.
49. Yim DG, Ahn DU, Nam KC. Effect of packaging and antioxidant combinations on physicochemical properties of irradiated restructured chicken rolls. Korean journal for food science of animal resources. 2015 Apr 30;35(2):248.
50. Popova T, Marinova P. Lipid oxidation in M. longissimus dorsi and M. semimembranosus in lambs reared indoors and on pasture. Iranian Journal of Applied Animal Science. 2013 Sep 1;3(3):533-7.
51. Grotta L, Castellani F, Palazzo F, Haouet MN, Martino G. Treatment optimisation and sample preparation for the evaluation of lipid oxidation in various meats through TBARs assays before analysis. Food Anal Methods 2017; 10: 1870–80.
52. Wereńska M, Okruszek A, Haraf G, Wołoszyn J, Goluch Z. Impact of frozen storage on oxidation changes of some components in goose meat. Poultry Science. 2022 Jan 1;101(1):101517.
53. Wang YH, Chen KC. Removal of Disinfection By-Products from Contaminated Water Using a Synthetic Goethite Catalyst via Catalytic Ozonation and a Biofiltration System·. International journal of environmental research and public health. 2014 Sep;11(9):9325-44.
54. Kanaan MH. Effectiveness of gaseous ozone on Arcobacter butzleri and bacterial loads on retailed meat sold at Iraqi Wasit markets. Open Veterinary Journal. 2024 Nov 30;14(11):2794.
55. Ghaffoori Kanaan MH, Razak A, Samir F. Effect of ozone treatment on thermo tolerant Campylobacter contaminated poultry products retailed at Baghdad markets/Iraq. Egyptian Journal of Nutrition. 2024 Jun 1;39(2):1-0.