بررسی اثر مهاری عصاره پولک (Stachys schtschegleevii) بر استرپتوکوکوس تولیدکننده بیوفیلم در شرایط آزمایشگاهی

نویسندگان
دانشگاه تبریز
چکیده
بیوفیلم‌ها به‌عنوان شکل طبیعی زندگی برای برخی از باکتری‌ها عمل می‌کنند، که در گونه‌های بیماری‌زا، عوامل حیاتی در ایجاد بیماری‌های عفونی می‌باشند. هدف از این تحقیق بررسی اثرات ضدمیکروبی عصاره Stachys schtschegleevii بر روی باکتری استرپتوکوکوس تولیدکننده بیوفیلم جداشده از سیستم آبرسانی مزارع صنعتی پرورش پولت تخمگذار بود. نتایج تولید بیوفیلم با روش صفحه کشت بافت (TCP) تعیین شد و نشان داد که هر دو گونه استرپتوکوکوس جداسازی شده توانایی تولید بیوفیلم را داشتند. به منظور بررسی فعالیت ضدمیکروبی، عصاره پولک در غلظت mg mL-1 200 و کمتر (با تهیه رقت سریالی) تهیه شد. ترکیبات زیست‌فعال عصاره که شامل محتوای فنولی کل، محتوای فلاونوئید کل و ظرفیت آنتی‌اکسیدانی بودند، به ترتیب mg GAE mL-1 83، mg QE mL-1 24 و 65% بدست آمدند. فعالیت ضدمیکروبی با بررسی حداقل غلظت مهاری (MIC) و حداقل غلظت باکتری‌کشی (MBC) عصاره اندازه‌گیری شد و با توجه به نتایج بدست آمده حداقل غلظت مهاری و حداقل غلظت باکتری‌کشی عصاره پولک برای استرپتوکوکوس مشتق شده از منبع ذخیره آب (B4) به ترتیب در غلظت 5/12و mg mL-1 25 و برای استرپتوکوکوس حاصل از سیستم آبرسانی سالن طیور (S2-6) به ترتیب در غلظت 25 و mg mL-1 50 مشاهده گردید. در این تحقیق تأثیر mg L-1 3 هیپوکلریت سدیم بر بقاء و رشد باکتری‌های تحت مطالعه به روش انتشار در چاهک آگار بررسی شد که نتایج حاکی از آن بود که استرپتوکوکوس جدا شده از سیستم آبرسانی سالن طیور (S2-6) در مقابل آن مقاوم بود در حالی که جدایه B4 نسبت به آن حساس بود.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigating the inhibitory effect of poulk extract (Stachys schtschegleevii) on biofilm forming Streptococcus in vitro

نویسندگان English

Hamed Jafarzadeh
Boukaga Farmani
University of Tabriz
چکیده English

Biofilms act as a natural form of life for some bacteria, which in pathogenic species are critical factors in causing infectious diseases. The aim of this research was to investigate the antimicrobial effects of Stachys schtschegleevii extract on biofilm forming Streptococcus isolated from the water supply system of industrial layer pullet farms. The results of biofilm production were determined by the tissue culture plate method and showed that both isolated Streptococcus species had the ability to produce biofilm. In order to investigate the antimicrobial activity of poulk extract, it was prepared at a concentration of 200 mg mL-1 and less (by preparing serial dilution). The bioactive compounds of the extract, which included total phenolic content, total flavonoid content, and antioxidant capacity, were obtained as 83 mg GAE mL-1, 24 mg QE mL-1, and 65%, respectively. Antimicrobial activity was measured by the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the extract for Streptococcus spp. that was obtained 12.5 and 25 mg mL-1 for the one derived from water storage source (B4) and 25 and 50 mg mL-1, respectively for Streptococcus obtained from water supply system of the poultry house (S2-6). In this research, the effect of 3 mg L-1 sodium hypochlorite on survival and growth of these bacteria was investigated by agar well diffusion method, and the results indicated that the Streptococcus isolated from water supply system of the poultry house (S2-6) was resistant to it while B4 isolate was sensitive.

کلیدواژه‌ها English

Biofilm
Bioactive compounds
Streptococci
Poulk extract
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