ترکیبات شیمیایی و خاصیت ضدقارچی اسانس زردچوبه (L. Curcuma longa)

نویسندگان
1 دانشجوی دکتری
2 عضو هیات علمی
چکیده
با افزایش تقاضای مصرف‌کنندگان جهت استفاده از محصولات غذایی فاقد نگهدارنده‌های شیمیایی استفاده از گیاهان دارویی و معطر با پتانسیل ضدمیکروبی و آنتی‌اکسیدانی بالا با تاثیر بر خواص شیمیایی محصولات غذایی رونق فزاینده‌ای را دارا بوده‌اند؛ بنابراین، در این پژوهش ترکیبات شیمیایی، میزان فنل و فلاونوئید کل اسانس زردچوبه مورد بررسی قرار گرفت، همچنین، خاصیت آنتی‌اکسیدانی با استفاده از آزمون‌های ABTS و DPPH و خاصیت ضدقارچی اسانس با استفاده از آزمون‌های دیسک دیفیوژن، چاهک آگار و تعیین حداقل غلظت مهار کنندگی و حداقل غلظت کشندگی بررسی شد. نتایج حاصل از آزمون کروماتوگرافی گازی متصل به طیف‌سنج جرمی نشان داد که β. Tumerone با میزان 43/43 % ترکیب شاخص این اسانس بود. میزان ترکیبات فنول کل این اسانس 053/130 میلی‌گرم گالیک اسید در هرگرم و میزان ترکیبات فلاونوئیدی کل آن برابر با 5/624 میلی‌گرم گالیک اسید در هرگرم کوئرستین مشاهده شد. میزان قابلیت ضدقارچی اسانس زردچوبه بر پنج گونه‌ی قارچی آسپرژیلوس نایجر، آسپرژیلوس فامیگاتوس، پنسیلیوم ایتالیکوم، پنیسیلیوم دیجیتاتوم و کاندیدا آلبیکنز مورد بررسی قرار گرفت که نتایج نشان دهنده‌ی توانایی خوب اسانس در مهارگونه‌ی کاندیدا آلبیکنز بوده است. باتوجه به نتایج به‌دست آمده، اسانس زردچوبه را می‌توان به عنوان یک ماده‌ی نگهدارنده‌ی طبیعی جایگزین مناسب برای نگهدارنده‌های شیمیایی دانست.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigation of chemical composition and antifungal properties of turmeric essential oil (Curcuma longa L.)

نویسندگان English

Noshin Jafarinejad 1
Hassan Barzegar 2
Mohammad Amin Mehrnia 2
Hossein Jooyandeh 2
Mohammad Hojjati 2
1 PhD student
2 Member of faculty
چکیده English

With the increasing consumer demand for chemical-free food products, the use of medicinal and aromatic plants with high antimicrobial and antioxidant potential has gained considerable attention, influencing the chemical properties of food products. In this study, the chemical compounds, total phenol, and flavonoid content of Curcuma longa essential oil were examined. Additionally, the antioxidant properties were assessed using ABTS and DPPH tests, and the antifungal properties of the essential oil were evaluated through disk diffusion, agar well diffusion, MIC and MBC methods. The results from the GC-MS analysis indicated that β-Turmerone was the major compound in the essential oil, accounting for 43.43%. The total phenolic content of the oil was 130.053 mg GAE/g, and the total flavonoid content was 624.5 5 mg QUE/g. The antifungal ability of Curcuma longa essential oil was tested against five fungal species: Aspergillus niger, Aspergillus fumigatus, Penicillium italicum, Penicillium digitatum, and Candida albicans. The results showed a strong inhibitory effect on Candida albicans. Based on the obtained results, Curcuma longa essential oil can be considered a promising natural preservative as an alternative to chemical preservatives.


کلیدواژه‌ها English

Antifungal agent
Chemical composition
Curcuma longa essential oil
1. Hu, Y., Zhang, J., Kong, W., Zhao, G., & Yang, M. (2017). Mechanisms of antifungal and anti-aflatoxigenic properties of essential oil derived from turmeric (Curcuma longa L.) on Aspergillus flavus. Food chemistry, 220, 1-8.‏
2. Ferreira, F. D., Mossini, S. A. G., Ferreira, F. M. D., Arrotéia, C. C., da Costa, C. L., Nakamura, C. V., & Machinski Junior, M. (2013). The inhibitory effects of Curcuma longa L. essential oil and curcumin on Aspergillus flavus link growth and morphology. The Scientific World Journal, 2013(1), 343804.‏
3. Novais, C., Molina, A. K., Abreu, R. M. V., Santo-Buelga, C., Ferreira, I. C. F. R., Pereira, C., & Barros, L. (2022). Natural Food Colorants and Preservatives: A Review, a Demand, and a Challenge. Journal of Agricultural and Food Chemistry, 70(9), 2789-2805.
4. Jokar, A., Barzegar, H., Maftoon Azad, N. & Shahamirian, M. (2021). Effects of cinnamon essential oil and Persian gum on preservation of pomegranate arils. Food Science and Nutrition. 9 (15): 2585-2596.
5. Ahmad, R. S., Hussain, M. B., Sultan, M. T., Arshad, M. S., Waheed, M., Shariati, M. A., ... & Hashempur, M. H. (2020). Biochemistry, safety, pharmacological activities, and clinical applications of turmeric: a mechanistic review. Evidence-based complementary and alternative medicine, 2020.‏
6. Nelson K, Dahlin J, Bisson J, et al. )2017(. The essential medicinal chemistry of curcumin. J Med Chem.; 60:1620–1637.
7. Abd El‐Hack, M. E., El‐Saadony, M. T., Swelum, A. A., Arif, M., Abo Ghanima, M. M., Shukry, M., ... & El‐Tarabily, K. A. (2021). Curcumin, the active substance of turmeric: its effects on health and ways to improve its bioavailability. Journal of the Science of Food and Agriculture, 101(14), 5747.‏
8. Singletary, K. (2020). Turmeric: potential health benefits. Nutrition Today, 55(1), 45-46.‏
9. Majdi, B., Mehrnia, M. A., Barzegar, H., & Alizadeh Behbahani, B. (2021). Determination of the structure, chemical composition, antioxidant activity and the cytotoxic effect of Turmeric essential oil. Iranian Food Science and Technology Research Journal, 17(2), 261-271
10. Mehrnia, M. A., Alizadeh Behbahani, B., Barzegar, H. & Tanavar, H. (2021). Sclerorhachis platyrachis essential oil: Antioxidant power, total phenolic and flavonoid content and its antimicrobial activity on some Gram-positive and Gram-negative bacteria “in vitro”. Journal of Food Science and Technology (Iran).112 (18): 189-198.
11. Majdi, B., Mehrnia, M. A., & Barzegar, H. (2022). Effect of turmeric (Curcuma longa L) essential oil on the oxidative stability of soybean oil. Journal of Food Science and Technology (Iran), 19(122), 335-348.
12. Namazi, P., Barzegar, H., Alizadeh Behbahani, B. & Mehrnia, M. A. (2021). Evaluation of functional groups of bioactive compounds, antioxidant potential, total phenolic and total flavonoid content of red bell pepper extracts. Journal of Food Science and Technology (Iran), 18(113):301-311.
13. Dehghan, N., Barzegar, H., Mehrnia, M. A., & Jooyandeh, H. (2018). Investigation on the effect of Methanolic Bene (pistachia atlantica) hull extract on oxidative stability of soybean oil. Innovative Food Technologies, 5(3), 499-507
14. Barzegar, H., Alizadeh Behbahani, B. & Noshad, M. (2021). Evaluation of total phenol and flavonoid contents, antioxidant and antimicrobial activity of Lawsonia inermis aqueous extract against some gram- positive and gram- negative bacteria. Journal of Food Science and Technology (Iran). 18(116): 327-335.
15. Kasta, G. (2020). Antimicrobial activity of ethanol extract of rhizome turmeric (Curcuma longa L.) for growth of Escherichia coli, Staphylococcus aureus and Candida albicans. Asian Journal of Pharmaceutical Research and Development, 8(3), 5-8.‏
16. Senouci, H., Benyelles, N. G., Dib, M. E., Costa, J., & Muselli, A. (2020). Chemical composition and combinatory antifungal activities of Ammoides verticillata, Allium sativum and Curcuma longa essential oils against four fungi responsible for tomato diseases. Combinatorial Chemistry & High Throughput Screening, 23(3), 196-204.‏
17. Murugesh, J., Annigeri, R. G., Mangala, G. K., Mythily, P. H., & Chandrakala, J. (2019). Evaluation of the antifungal efficacy of different concentrations of Curcuma longa on Candida albicans: An: in vitro: study. Journal of Oral and Maxillofacial Pathology, 23(2), 305.
18. Samiei, A., Tabatabaei- Yazdi, F‏. & Mazaheri, Tehrani, M. 2018. An investigation into the antioxidant activity, phenolic compounds, antimicrobial effect and interaction of the essential oils of Curcuma longa and Ocimum basilicum on some pathogenic bacteria. Journal of Food Science and Technology (Iran). 15(74): 99-107.
19. Ivanović, M., Makoter, K., & Islamčević Razboršek, M. (2021). Comparative study of chemical composition and antioxidant activity of essential oils and crude extracts of four characteristic Zingiberaceae herbs. Plants, 10(3), 501.‏
20. SARAÇ, H., & TÜZÜN, B. Antioxidant Activity Properties of Extract of Turmeric (Curcuma longa L.) Plant. Turkish Computational and Theoretical Chemistry, 8(2), 19-27.‏
21. Paw, M., Gogoi, R., Sarma, N., Pandey, S. K., Borah, A., Begum, T., & Lal, M. (2020). Study of anti-oxidant, anti-inflammatory, genotoxicity, and antimicrobial activities and analysis of different constituents found in rhizome essential oil of Curcuma caesia Roxb., collected from north east India. Current pharmaceutical biotechnology, 21(5), 403-413.‏
22. Samiei, A., Tabatabaei- Yazdi, F‏. & Mazaheri, Tehrani, M. 2018. Identification of Curcuma longa essential oil compounds and evaluation of its antibacterial effect on some food-poisoning bacteria “in vitro”. Journal of Food Science and Technology (Iran). 15(74): 321-329.
23. Akter, J., Hossain, M. A., Takara, K., Islam, M. Z., & Hou, D. X. (2019). Antioxidant activity of different species and varieties of turmeric (Curcuma spp): Isolation of active compounds. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 215, 9-17.‏
24. Khuntia, S., Lenka, J., Dash, M., Sahoo, B. C., Kar, B., & Sahoo, S. (2023). Bioactivity Screening of Thirty Black Turmeric (Curcuma caesia Roxb.) Essential Oils Against Free Radicals and MDR Isolates. Pharmacognosy Magazine, 19(3), 615-625.‏
25. De Carvalho, F. A. L., Munekata, P. E., de Oliveira, A. L., Pateiro, M., Dominguez, R., Terindade, M. A. and Lorenzo, J. M. (2020). Turmeric (curcuma longa L.) extract on oxidative stability, physicochemical and sensory properties of fresh lamb sausage with fat replace3ment by tiger nut (Cyperus esculentus L.) oil. Food Research International. 136: 109487.
26. Akinola, A. A., Ahmad, S., & Maziah, M. (2014). Total anti-oxidant capacity, flavonoid, phenolic acid and polyphenol content in ten selected species of Zingiberaceae rhizomes. African Journal of Traditional, Complementary and Alternative Medicines, 11(3), 7-13.‏
27. Chauhan, P., Keni, K., & Patel, R. (2017). Investigation of phytochemical screening and antimicrobial activity of Curcuma longa. Int J Adv Res Biol Sci, 4, 153-163.‏
28. Prakash, B., Singh, P., Kedia, A., Singh, A., & Dubey, N. K. (2012). Efficacy of essential oil combination of Curcuma longa L. and Zingiber officinale Rosc. as a postharvest fungitoxicant, aflatoxin inhibitor and antioxidant agent. Journal of Food Safety, 32(3), 279-288.‏