بررسی ویژگی‌های بیوشیمیایی و خاصیت ضدقارچی عصاره پوست انار با استفاده از حلال‌های مختلف

نویسندگان
الهام آذرپژوه 1
شهین زمردی 2
پروین شرایعی 3
1 1دانشیار پژوهشی بخش تحقیقات فنی و مهندسی مرکز تحقیقات، آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران.
2 دانشیار پژوهشی بخش تحقیقات فنی و مهندسی مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی آذربایجان غربی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه
3 دانشیار پژوهشی بخش تحقیقات فنی و مهندسی مرکز تحقیقات، آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران
10.22034/FSCT.22.162.223
چکیده
به ‌منظور بررسی ویژگی‌های بیوشیمیایی ترکیبات عصاره پوست انار، آزمایشی در قالب فاکتوریل بر پایه طرح کاملاً تصادفی انجام شد. فاکتورهای مورد بررسی شامل نوع حلال (آب، متانول 70 درصد و اتانول 70 درصد) و زمان استخراج (1، 12 و 24 ساعت) بود. نتایج نشان داد که قدرت احیا‌کنندگی آهن و فعالیت مهارکنندگی رادیکال آزاد در عصاره‌های استخراج شده با متانول 70 درصد بالاتر از سایر حلال‌ها بود. همچنین با افزایش زمان استخراج در تمام حلال‌های مورد استفاده، مقدار ترکیبات پلی‌فنلی، قدرت احیاکنندگی (FRAP) و قدرت مهارکنندگی رادیکال آزاد (DPPH) به طور معنی‌داری افزایش یافت که افزایش مقدار ترکیبات پلی‌فنلی و FRAP در استفاده از حلال آب بیشترین مقدار اما در حلال متانول کمترین مقدار بود. اما افزایش DPPH در استفاده از حلال اتانل بیشترین مقدار و در حلال متانول کمترین مقدار بود. نتایج حاصل از بررسی اثرات ضد قارچی عصاره متانولی پوست انار نشان داد که عصاره متانولی پوست انار با غلظت 3/0 درصد، دارای خاصیت ضدقارچی تقریباً معادل با سوربات پتاسیم با غلظت 1/0 درصد بود، به طوری که میانگین قطر هاله عدم رشد کپک آسپرژیلوس نایجر تحت تاثیر عصاره پوست انار 7/17 میلی‌متر و تحت تاثیر سوربات پتاسیم 3/18 میلی‌متر بود. لذا عصاره پوست انار می‌تواند به عنوان ترکیبات آنتی‌اکسیدنی و نگهدارنده طبیعی، جایگزین مناسبی برای نگهدارنده‌های شیمیایی مانند سوربات پتاسیم در محصولات غذایی استفاده شود.
کلیدواژه‌ها
: انار
استخراج
حلال
ترکیبات موثره
خواص ضد قارچ

موضوعات

عنوان مقاله English

Investigation of Biochemical Properties and Antifungal Activity of Pomegranate Peel Extract Using Different Solvents

نویسندگان English

Elham Azarpazhooh 1
Shahin Zomorodi 2
Parvin Sharayei 3
1 Agricultural Engineering Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran
2 Agricultural Engineering Research Department, West Azerbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Urmia
3 Agricultural Engineering Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran
چکیده English

The results showed that the Ferric reducing activity of plasma (RRAP) and free radical scavenging activity (DPPH) in extracts obtained with 70% methanol were higher than those with other solvents. Also, in water and methanol solvents, with increasing extraction time, the amount of polyphenol compounds and FRAP were the highest and the lowest, respectively, but the increase of DPPH was the highest in ethanol solvent and the lowest in methanol solvent. The results of the investigation of the antifungal effects of the methanol extract of pomegranate peel (MEPP) showed that this extract with a concentration of 0.3% had an antifungal effect nearly equivalent to that of potassium sorbate at a concentration of 0.1%, so that the mean diameter on the diameter of Aspergillus niger growth inhibition zone was 17.7 mm for pomegranate peel extract and 18.3 mm for potassium sorbate. Therefore, pomegranate peel extract can be used in food products as antioxidant compounds and preservatives as a natural and effective alternative to chemical preservatives like potassium sorbate.

کلیدواژه‌ها English

Pomegranate
Extraction
solvent
Effective Compounds
Antifungal properties
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