ارزیابی اثر حلال ها و روش های استخراج بر بازده ی استخراج، تعیین مقدار مواد معدنی و فعالیت آنتی باکتری عصاره ی گل گیاه اویار سلام(Cyperus Rotundus L.)

نویسندگان
1 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران
2 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایرا
3 فارغ التحصیل مهندسی شیمی دانشگاه آزاد ماهشهر
چکیده
گیاه اویارسلام (Cyperus Rotundus L.) از تیره ی جگن (Lyperaceae ) و یک علف هرز تک لپه ای می باشد. غده گیاه اویارسلام دارویی خانگی برای سوء هاضمه، استفراغ، اسهال، تلخ، قابض، آرام بخش، بادشکن، مدر، کرم کش، قاعده آور و مقوی اعصاب است. در این مطالعه برای اولین بار از گل گیاه اویار سلام به دو روش ماسراسیون و فراصوت با حلال های آب مقطر و اتانول و در زمان های 15 و 30 دقیقه عصاره گیری شد. هم چنین فعالیت آنتی باکتری با روش تعیین قطر هاله ی عدم رشد،حداقل غلظت بازدارندگی رشد(MIC) و حداقل غلظت باکتری کشی(MBC) و مقدار مواد معدنی موجود در عصاره ی گل مورد ارزیابی قرار گرفت. نتایج بدین صورت گزارش شد که تفاوت بین بازده ی استخرج هر دو روش ناچیز است ولی روش فراصوت به دلیل کاهش بازه ی زمانی عصاره گیری نسبت به ماسراسیون بهینه تر می باشد. در بین مقادیر مواد معدنی بیش ترین مقدار برای پتاسیم و کم ترین مقدار برای مس، مولیبدن و سلنیوم حاصل شد. نتایج حاصل از بررسی خاصیّت آنتی باکتری عصاره ی گل نسبت به آنتی بیوتیک نشان دهنده ی این است که اثر مهارکنندگی آنتی بیوتیک ها روی باکتری اشریشیاکلی و استافیلوکوکوس اورئوس بیش تر است.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Evaluating the effect of solvents and extraction methods on extraction yield, determining the amount of minerals and antibacterial activity of Cyperus Rotundus L flower extract

نویسندگان English

Asana Ahmadi 1
Reza Naghiha 2
Abolfazl Ahmadi 3
1 department of pathobiology، Faculty of Veterinary Medicine، shahid chamran university of ahvaz، ahvaz،iran
2 Former associate professor of Yasouj University
3 Graduated in Chemical Engineering from Mahshahr Azad University
چکیده English

Cyperus Rotundus L plant is a monocotyledonous weed from the Lyperaceae family. Cyperus Rotundus L of tuber is a home remedy for indigestion, vomiting, diarrhea, bitter, astringent, sedative, carminative, diuretic, anthelmintic, menstruate and nerve tonic. In this study, for the first time, the flower of Cyperus Rotundus L plant was extracted by two methods of maceration and ultrasound with distilled water and ethanol solvents and at times of 15 and 30 minutes. Also, the antibacterial activity was evaluated by determining the diameter of growth inhibition zone, the minimum growth inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) and the amount of minerals in the flower extract. The results were reported that the difference between the extraction yield of both methods is insignificant, but the ultrasonic method is more optimal than maceration due to the reduction of the extraction time. Among the amounts of minerals, the highest amount was obtained for potassium and the lowest amount for copper, molybdenum and selenium. The results of investigating the antibacterial property of flower extract compared to antibiotics show that the inhibitory effect of antibiotics on Escherichia coli and Staphylococcus aureus bacteria is more.

کلیدواژه‌ها English

Cyperus Rotundus L
Flower
Extract
Extraction yield
Minerals
Antibacterial activity
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