بررسی ویژگی های شیمیایی و ضدمیکروبی اسانس بابونه بهاری بر باکتری‌های سالمونلا تیفی‌موریوم، انتروباکتر ائروژنز، استافیلوکوکوس اورئوس، شیگلا دیسانتری، باسیلوس سرئوس و استرپتوکوکوس پیوژنز

نویسندگان
محمدامین مهرنیا
بهروز علیزاده بهبهانی
محمد نوشاد
1. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.
10.22034/FSCT.22.159.43
چکیده
این مطالعه با هدف استخراج اسانس از گیاه دارویی بابونه بهاری (Anthemis cotula) و بررسی میزان فنول کل، فلاونوئید کل، اثر آنتی‌اکسیدانی و فعالیت ضدمیکروبی آن صورت پذیرفت. بررسی محتوای فنول کل اسانس با کمک روش فولین سیوکالتو نشان داد که اسانس دارای mg GAE/g 80/29 فنول کل می‌باشد. محتوای فلاونوئید کل اسانس برابر با mg QE/g 47/13 بود. بررسی اثر آنتی‌اکسیدانی اسانس با کمک روش‌های مهار رادیکال آزاد DPPH و ABTS نشان داد که اسانس دارای فعالیت آنتی‌اکسیدانی قابل‌توجهی می‌باشد. اثر آنتی‌اکسیدانی در برابر رادیکال‌های آزاد DPPH و ABTS به ترتیب برابر با 80/53 و 60/55 درصد به دست آمد. ارزیابی فعالیت ضدمیکروبی اسانس در برابر باکتری‌های سالمونلا تیفی­موریوم، انتروباکتر ائروژنز، استافیلوکوکوس اورئوس، شیگلا دیسانتری، باسیلوس سرئوس و استرپتوکوکوس پیوژنز بر اساس روش‌های دیسک دیفیوژن آگار، چاهک آگار و حداقل غلظت مهارکنندگی و کشندگی نشان داد که فعالیت ضد باکتریایی اسانس قابل‌توجه است. باکتری‌های­ استافیلوکوکوس اورئوس و شیگلا دیسانتری به ترتیب با بالاترین و کمترین قطر هاله عدم رشد، حساس‌ترین و مقاوم‌ترین سویه‌ها در برابر اسانس بودند. نتایج نشان داد که اسانس بابونه بهاری منبع طبیعی ترکیبات فعال با اثرات آنتی‌اکسیدانی و ضد باکتریایی در برابر باکتری‌های پاتوژن می‌باشد.
کلیدواژه‌ها
اسانس
اثر آنتی‌اکسیدانی
فعالیت ضدمیکروبی
بابونه بهاری
ترکیبات زیست فعال

موضوعات

عنوان مقاله English

Investigating the chemical and antimicrobial properties of Anthemis cotula essential oil against Salmonella typhimurium, Enterobacter aerogenes, Staphylococcus aureus, Shigella dysentery, Bacillus cereus and Streptococcus pyogenes

نویسندگان English

Mohammad Amin Mehrnia
Behrooz Alizadeh Behbahani
Mohammad Noshad
1Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
چکیده English

This study aimed to extract essential oil from the medicinal plant Anthemis cotula (Mayweed) and evaluate its total phenolic content, total flavonoids, antioxidant properties, and antimicrobial activity. The analysis of total phenolic content in the essential oil using the Folin-Ciocalteu method revealed a value of 29.80 mg GAE/g. Additionally, the total flavonoid content was determined to be 13.47 mg QE/g. The essential oil exhibited significant antioxidant activity based on its ability to scavenge free radicals using the DPPH and ABTS methods. The antioxidant activity against DPPH and ABTS radicals was found to be 53% and 55%, respectively. Furthermore, the antimicrobial activity of the essential oil was assessed against various pathogenic bacteria, including Salmonella typhimurium, Enterobacter aerogenes, Staphylococcus aureus, Shigella dysenteriae, Bacillus cereus, and Streptococcus pyogenes. The disk diffusion agar, well diffusion agar, and minimum inhibitory concentration methods demonstrated that the essential oil exhibited remarkable antibacterial activity. Among the tested strains, Staphylococcus aureus and Shigella dysenteriae were the most sensitive and resistant, respectively, to the essential oil. These findings highlight that Mayweed essential oil serves as a natural source of bioactive compounds with antioxidant and antibacterial effects against pathogenic bacteria.

کلیدواژه‌ها English

Essential oil
antioxidant effects
Antimicrobial activity
Mayweed
Bioactive compounds
[1] Tabatabaei Yazdi, F., Alizadeh Behbahani, B., Vasiee, A., Mortazavi, S. A. , & Yazdi, F. T. (2015). An investigation on the effect of alcoholic and aqueous extracts of Dorema aucheri (Bilhar) on some pathogenic bacteria in vitro. Archives of Advances in Biosciences, 6(1), 58-64.
[2] Shirani, K., Falah, F., Vasiee, A., Yazdi, F. T., Behbahani, B. A. , & Zanganeh, H. (2022). Effects of incorporation of Echinops setifer extract on quality, functionality, and viability of strains in probiotic yogurt. Journal of Food Measurement and Characterization, 16(4), 2899-2907.
[3] Tabatabaei Yazdi, F., Falah, F., Alizadeh Behbahani, B., Vasiee, A. , & Mortazavi, A. (2019). Antimicrobial effect of Citrus aurantium essential oil on some food-borne pathogens and its determination of chemical compounds, total phenol content, total flavonoids content and antioxidant potential. Journal of Food Science and Technology, 16(87), 291-304.
[4] Alizadeh Behbahani, B., Noshad, M. , & Falah, F. (2020). The combined effect of the combined Fennel and Clove essential oils on Staphylococcus epidermidis, Bacillus cereus, Salmonella typhi and Enterobacter aerogenes using Checkerboard assay (fractional inhibitory concentration index). Journal of Food Science and Technology, 17(106), 75-83.
[5] Alizadeh Behbahani, B., Yazdi, F. T., Mortazavi, A., Gholian, M. M., Zendeboodi, F. , & Vasiee, A. (2014). Antimicrobial effect of Carboxy Methyl Cellulose (CMC) containing aqueous and ethanolic Eucalyptus camaldulensis L. leaves extract against Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis. Archives of Advances in Biosciences, 5(2), 59-69.
[6] Yazdi, F. T., Falah, F., Behbahani, B. A., Vasiee, A. , & Mortazavi, S. A. (2019). Identification of Chemical Compounds, Antioxidant Potential, Phenolic Content and Evaluation of Inhibitory and Bactericidal/Fungicidal Effects of Ginger Essential Oil on Some Pathogenic Microorganisms in Vitro. Qom University of Medical Sciences Journal 13(3), 50-62.
[7] Yazdi, F. T., Tanhaeian, A., Azghandi, M., Vasiee, A., Alizadeh Behbahani, B., Mortazavi, S. A. , & Roshanak, S. (2019). Heterologous expression of Thrombocidin-1 in Pichia pastoris: Evaluation of its antibacterial and antioxidant activity. Microbial Pathogenesis, 127, 91-96.
[8] Sureshjani, M. H., Yazdi, F. T., Mortazavi, S. A., Behbahani, B. A. , & Shahidi, F. (2014). Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria" in vitro. Journal of Paramedical Sciences, 5(2), 115-120.
[9] Behbahani, B. A., Shahidi, F., Yazdi, F. T. , & Mohebbi, M. (2013). Antifungal effect of aqueous and ethanolic mangrove plant extract on pathogenic fungus" in vitro". International Journal of Agronomy and Plant Production, 4(7), 1652-1658.
[10] Yazdi, F. T. , & Behbahani, B. A. (2013). Antimicrobial effect of the aqueous and ethanolic Teucrium polium L. extracts on gram positive and gram negative bacteria “in vitro”. Archives of Advances in Biosciences, 4(4), 56-62.
[11] Albayrak, S. , & Aksoy, A. (2013). Evaluation of Antioxidant and Antimicrobial Activities of Two Endemic Anthemis Species in Turkey. Journal of Food Biochemistry, 37(6), 639-645.
[12] Falah, F., Shirani, K., Vasiee, A., Yazdi, F. T. , & Behbahani, B. A. (2021). In vitro screening of phytochemicals, antioxidant, antimicrobial, and cytotoxic activity of Echinops setifer extract. Biocatalysis and Agricultural Biotechnology, 35, 102102.
[13] Alizadeh Behbahani, B., Falah, F., Vasiee, A. , & Tabatabaee Yazdi, F. (2021). Control of microbial growth and lipid oxidation in beef using a Lepidium perfoliatum seed mucilage edible coating incorporated with chicory essential oil. Food science & nutrition, 9(5), 2458-2467.
[14] Heydari, S., Jooyandeh, H., Alizadeh Behbahani, B. , & Noshad, M. (2020). The impact of Qodume Shirazi seed mucilage‐based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study. Food Science & Nutrition, 8(12), 6497-6512.
[15] Tanavar, H., Barzegar, H., Alizadeh Behbahani, B. , & Mehrnia, M. A. (2021). Investigation of the chemical properties of Mentha pulegium essential oil and its application in Ocimum basilicum seed mucilage edible coating for extending the quality and shelf life of veal stored in refrigerator (4°C). Food Science & Nutrition, 9(10), 5600-5615.
[16] Jalil Sarghaleh, S., Alizadeh Behbahani, B., Hojjati, M., Vasiee, A. , & Noshad, M. (2023). Evaluation of the constituent compounds, antioxidant, anticancer, and antimicrobial potential of Prangos ferulacea plant extract and its effect on Listeria monocytogenes virulence gene expression [Original Research]. Frontiers in Microbiology, 14.
[17] Oliveira, L. d. L. d., Carvalho, M. V. d. , & Melo, L. (2014). Health promoting and sensory properties of phenolic compounds in food. Revista Ceres, 61, 764-779.
[18] Cosme, P., Rodríguez, A. B., Espino, J. , & Garrido, M. (2020). Plant phenolics: Bioavailability as a key determinant of their potential health-promoting applications. Antioxidants, 9(12), 1263.
[19] Halliwell, B., Rafter, J. , & Jenner, A. (2005). Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not? The American journal of clinical nutrition, 81(1), 268S-276S.
[20] Lo'ay, A., Abu-Orabi, S. T., Hlail, H. M., Alkhatib, R. Q., Al-Dalahmeh, Y. , & Al-Qudah, M. A. (2023). Anthemis cotula L. from Jordan: Essential oil composition, LC-ESI-MS/MS profiling of phenolic acids-flavonoids and in vitro antioxidant activity. Arabian Journal of Chemistry, 16(2), 104470.
[21] Sabeghi, Y., Varidi, M. , & Nooshkam, M. (2024). Bioactive foamulsion gels: a unique structure prepared with gellan gum and Acanthophyllum glandulosum extract. Journal of the Science of Food and Agriculture, 10.1002/jsfa.13267.
[22] Panda, S. S., Sahoo, A., Das, K. T., Jena, S., Ray, A., Das, P. K., Nayak, S. , & Panda, P. C. (2023). Chemical composition, antioxidant, antimicrobial and antiproliferative activities of the leaf essential oil of Roxburgh’s Cherry (Eugenia roxburghii), an underutilized species. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 1-12.
[23] Noshad, M., Hojjati, M. , & Behbahani, B. A. (2018). Black Zira essential oil: Chemical compositions and antimicrobial activity against the growth of some pathogenic strain causing infection. Microbial pathogenesis, 116, 153-157.
[24] Barzegar, H., Alizadeh Behbahani, B. , & Mehrnia, M. A. (2020). Quality retention and shelf life extension of fresh beef using Lepidium sativum seed mucilage-based edible coating containing Heracleum lasiopetalum essential oil: an experimental and modeling study. Food Science and Biotechnology, 29(5), 717-728.
[25] Romanova, D., Vachalkova, A., Cipak, L., Ovesna, Z. , & Rauko, P. (2001). Study of antioxidant effect of apigenin, luteolin and quercetin by DNA protective method. Neoplasma, 48(2), 104-107.
[26] Babaei, F., Moafizad, A., Darvishvand, Z., Mirzababaei, M., Hosseinzadeh, H. , & Nassiri‐Asl, M. (2020). Review of the effects of vitexin in oxidative stress‐related diseases. Food science & nutrition, 8(6), 2569-2580.
[27] Victoria, F. N., Lenardão, E. J., Savegnago, L., Perin, G., Jacob, R. G., Alves, D., da Silva, W. P., da Motta, A. d. S. , & da Silva Nascente, P. (2012). Essential oil of the leaves of Eugenia uniflora L.: antioxidant and antimicrobial properties. Food and chemical toxicology, 50(8), 2668-2674.
[28] Yue, L., Wang, S., Xie, Y., Hou, Z., Liu, L. , & Zhang, Y. (2021). Study on Antioxidant Activity of Anthemis nobilis Extract in Liquid. IOP Conference Series: Earth and Environmental Science, 792(1), 012011.
[29] Huiwen, T., Bingting, C., Yilizere, A., Delong, L. , & Xiaoli, M. (2022). Monosaccharide composition and antioxidant activity of polysaccharides from Matricaria chamomilia and Anthemis nobilis. China Food Additives, 33(2).
[30] Djihane, B., Wafa, N., Elkhamssa, S., Maria, A. E. , & Mihoub, Z. M. (2017). Chemical constituents of Helichrysum italicum (Roth) G. Don essential oil and their antimicrobial activity against Gram-positive and Gram-negative bacteria, filamentous fungi and Candida albicans. Saudi Pharmaceutical Journal, 25(5), 780-787.
[31] Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Noorbakhsh, H., Riazi, F., Jajarmi, A. , & Tabatabaei Yazdi, F. (2016). Study of the antibacterial activity of methanolic and aqueous extracts of Myrtus communis on pathogenic strains causing infection. Zahedan Journal of Research in Medical Sciences, 18(2), e5989.
[32] Quarenghi, M., Tereschuk, M., Baigori, M. , & Abdala, L. (2000). Antimicrobial activity of flowers from Anthemis cotula. Fitoterapia, 71(6), 710-712.
[33] Bardaweel, S. K., Tawaha, K. A. , & Hudaib, M. M. (2014). Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil. BMC Complementary and Alternative Medicine, 14, 1-8.