غربالگری فاکتورهای موثر در رشد باکتری Paenibacillus polymyxa با استفاده از طرح آزمایشی پلاکت-برمن

نویسندگان
علیرضا وسیعی 1
محبوبه سرابی 2
فرشته فلاح 3
سیدعلی مرتضوی 3
1 گروه ایمنی و کنترل کیفیت مواد غذایی، مؤسسه پژوهشی علوم و صنایع غذایی، مشهد، ایران
2 گروه زیست فناوری مواد غذایی، مؤسسه پژوهشی علوم و صنایع غذایی، مشهد، ایران
3 گروه علوم ومهندسی صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
10.22034/FSCT.22.158.213
چکیده
باکتری Paenibacillus polymyxa از جمله میکروارگانیسم­های است که توانایی تولید اگزوپلی­ساکاریدهای خارج سلولی و نیز آنتی­بیوتیک­ها را دارا می­باشد. عوامل متعددی از جمله محتوای محیط کشت، منبع کربن و نیتروژن، pH، دما، سرعت هوا و شرایط کشت بر رشد و تولید توده سلولی بالاتر و نیز تولید متابولیت­های میکروبی تأثیر دارند. هدف از این مطالعه بررسی میزان رشد باکتری P. polymyxa در محیط کشت حاوی ملاس و همچنین غربالگری 5 جزء محیط کشت و 4 فاکتور شرایط تخمیر با استفاده از روش پلاکت-برمن جهت به حداکثر رساندن میزان تولید توده سلولی بود. نتایج نشان داد از بین متغیرهای مورد بررسی بریکس ملاس مورد استفاده، زمان، درصد تلقیح، میزان سولفات آمونیوم، سرعت هم­زدن، میزان گلوکز و اوره به‌صورت معادله درجه اول اثر مثبت معنی‌داری بر رشد باکتری و تولید زیست­توده دارد که از این میان اثر بریکس ملاس مورد استفاده بیش از سایر متغیرها است؛ این درحالی است که pH و میزان عناصر کم مصرف مورد استفاه اثر منفی بر رشد سلولی داشتند. نتایج این مطالعه نشان داد محیط کشت برپایه ملاس می­تواند به عنوان یک گزینه ارزان قیمت و مناسب برای رشد باکتری P. polymyxa مورد استفاده قرار گیرد.
کلیدواژه‌ها
: Paenibacillus polymyxa
رشد سلولی
پلاکت برمن

موضوعات

عنوان مقاله English

Screening of effective factors in the growth of Paenibacillus polymyxa using Plackett - Burman experimental desig

نویسندگان English

Alireza Vasiee 1
Mahboobe Sarabi-Jamab 2
Fereshteh Falah 3
Seyed Ali Mortazavi 3
1 Department of Food Safety and Quality Control, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran
2 Department of Food Biotechnology, Research Institute of Food Science and Technology, RIFST, Mashhad, Iran
3 Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده English

Paenibacillus polymyxa is one of the microorganisms that has the ability to produce extracellular exopolysaccharides and antibiotics. Several factors, including culture medium content, carbon and nitrogen sources, pH, temperature, air velocity, and culture conditions, have an effect on the growth and production of higher cell mass, as well as the production of microbial metabolites. The purpose of this study was to investigate the growth rate of P. polymyxa in a culture medium containing molasses and to screen five components of the culture medium along with four factors of the fermentation conditions using the Plackett -Burman method to maximize cell mass production. The results showed that among the investigated variables, molasses brix, time, percentage of inoculation, amount of ammonium sulfate, stirring speed, and the amount of glucose and urea, as a first-order equatino, had a significant positive effect on bacterial growth and biomass production. Molasses brix medium was found to be more effective than other variables; however, pH and the amount of low-use elements had a negative effect on cell growth. The findings of this study indicated that molasses-based culture medium can be used as a cost-effective and suitable option for the growth of P. polymyxa.


کلیدواژه‌ها English

Paenibacillus polymyxa
cell growth
Plackett -Burman
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