PHYSICO-CHEMICAL CHARACTERIZATION OF ALPHA AMYLASE FROM BACILLUS SUBTILIS IMD34 USING FOOD WASTE AS SUBSTRATE

نویسنده
Effiom Henshaw
گروه میکروبیولوژی دانشگاه کالابار
10.22034/FSCT.21.155.121
چکیده
Amylase are the most important group of industrial enzymes that hydrolyze starch into amylopectin. The industrial demand of amylase predominantly from the microbial origin has been recently increased and enhances the research for amylase with high stability at extreme industrial conditions. Thus, this study is aimed to characterize amylase from Bacillus subtilis IMD34 obtained from previous using agro waste. Extracellular amylase production was carried out in basal medium by submerged fermentation technique. The production medium with 10% potato peels at pH 6.8 and 35 °C enhanced amylase activity (720 U/mL) than other wastes such as banana peels and yam peels. This optimized media increased the bacterial growth rate and activity of amylase compared to the non-supplemented basal medium. Further, amylase was purified and assessed their molecular weight (~39 k Da) in 12 % SDS -PAGE. The enzyme activity was observed at pH 6.8 and 35°C and stable over a wide range of pH (4-10), and temperatures (20-50°C). In addition, enzyme activity was stimulated in the presence of Mg2+, Ca2+ and Mn2+ and was unpretentious after treating with surfactants (SDS, Triton X-100, Tween 80), organic solvents (ethanol, methanol, chloroform and acetone) and amylase inhibitors (EDTA and β-mercaptoethanol). These enzymic characteristics makes the amylase obtained in this study a promising candidate for various industrial sector application.
کلیدواژه‌ها
Bacillus subtilis IMD34
Agro-waste and physico-chemical characteristics

موضوعات

عنوان مقاله English

PHYSICO-CHEMICAL CHARACTERIZATION OF ALPHA AMYLASE FROM BACILLUS SUBTILIS IMD34 USING FOOD WASTE AS SUBSTRATE

نویسنده English

Effiom Henshaw
Microbiology Department University of Calabar
چکیده English

Amylase are the most important group of industrial enzymes that hydrolyze starch into amylopectin. The industrial demand of amylase predominantly from the microbial origin has been recently increased and enhances the research for amylase with high stability at extreme industrial conditions. Thus, this study is aimed to characterize amylase from Bacillus subtilis IMD34 obtained from previous using agro waste. Extracellular amylase production was carried out in basal medium by submerged fermentation technique. The production medium with 10% potato peels at pH 6.8 and 35 °C enhanced amylase activity (720 U/mL) than other wastes such as banana peels and yam peels. This optimized media increased the bacterial growth rate and activity of amylase compared to the non-supplemented basal medium. Further, amylase was purified and assessed their molecular weight (~39 k Da) in 12 % SDS -PAGE. The enzyme activity was observed at pH 6.8 and 35°C and stable over a wide range of pH (4-10), and temperatures (20-50°C). In addition, enzyme activity was stimulated in the presence of Mg2+, Ca2+ and Mn2+ and was unpretentious after treating with surfactants (SDS, Triton X-100, Tween 80), organic solvents (ethanol, methanol, chloroform and acetone) and amylase inhibitors (EDTA and β-mercaptoethanol). These enzymic characteristics makes the amylase obtained in this study a promising candidate for various industrial sector application.

کلیدواژه‌ها English

Bacillus subtilis IMD34
Agro-waste and physico-chemical characteristics
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