The Inhibitory Effect of Prosopis juliflora Pods Protein Hydrolysate on Polyphenol Oxidase and Browning of Apple Slices During Refrigerated Storage

نویسنده
دانشگاه تهران
چکیده
The study was conducted exploit the proteins of the pods of some types of plants, such as Prosopis juliflora pods, that are considered by-products in many countries and considered a good source of protein in the preparation of protein hydrolysers and evaluating their effect on inhibiting brown discoloration of apple slices and compared with anti-browning agents (ascorbic acid, acetic acid, and sodium chloride) when stored in the refrigerator for 0, 5 and 8 days. The chemical composition of the moisture, protein, fat, ash, and carbohydrate of the P. juliflora pods was estimated, then the hydrolysis process was carried out using the enzymes trypsin and papain for 300 minutes. Amino acids and FTIR analysis of protein hydrolysates were determined. Significant changes (p≤0.05) in pH, total soluble solids, and non-significant changes in titration acidity of apple slices treated with protein hydrolysis and anti-browning agents were studied and significantly decreased (p≤0.05) in the activity of polyphenol oxidase until the end of the storage. The brown coloration decreased when treated with protein hydrolysates compared to other treatments, but non-significant changes. As a result, apple slices can be preserved with protein hydrolysers for several days in the refrigerator.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

The Inhibitory Effect of Prosopis juliflora Pods Protein Hydrolysate on Polyphenol Oxidase and Browning of Apple Slices During Refrigerated Storage

نویسنده English

Hadi Razavi
University of tehran
چکیده English

The study was conducted exploit the proteins of the pods of some types of plants, such as Prosopis juliflora pods, that are considered by-products in many countries and considered a good source of protein in the preparation of protein hydrolysers and evaluating their effect on inhibiting brown discoloration of apple slices and compared with anti-browning agents (ascorbic acid, acetic acid, and sodium chloride) when stored in the refrigerator for 0, 5 and 8 days. The chemical composition of the moisture, protein, fat, ash, and carbohydrate of the P. juliflora pods was estimated, then the hydrolysis process was carried out using the enzymes trypsin and papain for 300 minutes. Amino acids and FTIR analysis of protein hydrolysates were determined. Significant changes (p≤0.05) in pH, total soluble solids, and non-significant changes in titration acidity of apple slices treated with protein hydrolysis and anti-browning agents were studied and significantly decreased (p≤0.05) in the activity of polyphenol oxidase until the end of the storage. The brown coloration decreased when treated with protein hydrolysates compared to other treatments, but non-significant changes. As a result, apple slices can be preserved with protein hydrolysers for several days in the refrigerator.

کلیدواژه‌ها English

Protein hydrolysates
Polyphenol oxidase
Browning
Inhibitory
Apple slices
Ali, M. T., Mehraj, S., Mir, M. S., Shah, I. A., Shah, Z. A., El-Serehy, H. A. Shafik, H. M. 2023. Deciphering the response of thirteen apple cultivars for growth, fruit morphology and fruit physico-chemical attributes during different years. Heliyon. 9(8). https://doi.org/10.1016/j.heliyon.2023.e17260.
Arteagaa, V.G.; Guardiab ,M. A.; Muranyi, I.; Eisnerb,P. and Schweiggert-Weiszb,U. 2020. Effect of enzymatic hydrolysis on molecular weight distribution, technofunctional properties and sensory perception of pea protein isolates. Innovative Food Science and Emerging Technologies.65:102449. https://doi.org/10.1016/j.ifset.2020.102449.
Brosnan, J. T. and Brosnan, M. E. 2006. The sulfur-containing amino acids: an overview. Journal of Nutrition 136: 1636S-1640S.
De Lemos, A. B. S., Chaves, G., Ribeiro, P. P. C., da Silva Chaves Damasceno, K. S. F. 2023. Prosopis juliflora: nutritional value, bioactive activity, and potential application in human nutrition. Journal of the Science of Food and Agriculture, 103(12), 5659-5666. https://doi.org/10.1002/jsfa.12620.
De Meutter, J., Goormaghtigh, E. 2021. Evaluation of protein secondary structure from FTIR spectra improved after partial deuteration. European Biophysics Journal, 50, 613-628. https://doi.org/10.1007/s00249-021-01502-y.
Garg, D., Chakraborty, S., Gokhale, J. S. 2020. Optimizing the extraction of protein from Prosopis cineraria seeds using response surface methodology and characterization of seed protein concentrate. LWT, 117, 108630. https://doi.org/10.1016/j.lwt.2019.108630.
Gayathri, G., Uppuluri, K. B. 2022. The comprehensive characterization of Prosopis juliflora pods as a potential bioenergy feedstock. Scientific Reports, 12(1), 18586. https://doi.org/10.1038/s41598-022-22482-9.
Girelli, A. M., Mattei, E., Messina, A., Tarola, A. M. 2004. Inhibition of polyphenol oxidases activity by various dipeptides. Journal of agricultural and food chemistry, 52(10), 2741-2745. https://doi.org/10.1021/jf0305276.
Gonzales-Barron, U. ,. Dijkshoorn, R., Maloncy M. 2020. “Nutritive and bioactive properties of mesquite (Prosopis pallida) flour and its technological performance in breadmaking,” Foods, vol. 9, no. 5, p. 597. https://doi.org/10.3390/foods9050597.
Hoyle, N.T. and Merrit, J.H. 1994. Quality of fish protein hydrolysate from Herring (Clupea harengus). J. Food Sci 59:76–79. https://doi.org/10.1111/j.1365-2621.1994.tb06901.x.
Hsu, B. L., Weng, Y. M., Liao, Y. H., Chen, W. 2005. Structural investigation of edible zein films/coatings and directly determining their thickness by FT-Raman spectroscopy. Journal of Agricultural and Food Chemistry, 53(13), 5089-5095. https://doi.org/10.1021/jf0501490.
Kathirvel, P., Kumudha, P. 2011. Chemical composition of prosopis juliflora (SW.) DC (mosquito bean). Int. J. Appl. Biol. Pharmaceut. Technol, 2(4), 5-14.
Levin, S. and Grushka, E. 1985. Reversed-phase liquid chromatographic separation of amino acids with aqueous mobile phases containing copper ions and alkylsulfonates. Anal. Chem., 57(9): 1830-1835. https://doi.org/10.1021/ac00286a011.
Moon, K. M., Kwon, E. B., Lee, B., & Kim, C. Y. 2020. Recent trends in controlling the enzymatic browning of fruit and vegetable products. Molecules, 25(12), 2754. https://doi.org/10.3390/molecules25122754.
Morais, H.A.; Silvestre, M.P.C.; Silva, V.D.M.; Silva, M.R.; Silva, A.C.S. and Silvera, J.A. 2013. Correlation between the degree of hydrolysis and the peptide profile of whey protein concentrate hydrolysates: Effect of the enzyme type and reaction time. American Journal of Food Technology, 8(1): 315-324. https://doi.org/10.3923/ajft.2013.1.16.
Sai, K. P., & Babu, M. (2001). Studies on Rana tigerina skin collagen. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 128(1), 81-90. https://doi.org/10.1016/S1096-4959(00)00301-8.
Schurink, M.; van Berkel, W. J. H.; Wichers, H. J. and Boeriu, C. G. 2007. Novel peptides with tyrosinase inhibitory activity. Peptides 28: 485-495. https://doi.org/10.1016/j.peptides.2006.11.023.
Sciammaro, L., Ferrero, C., & Puppo, M. C. 2016. Chemical and nutritional properties of different fractions of Prosopis alba pods and seeds. Journal of Food Measurement and Characterization, 10, 103-112. https://doi.org/10.1007/s11694-015-9282-z.
Shrestha, L., Kulig, B., Moscetti, R., Massantini, R., Pawelzik, E., Hensel, O., Sturm, B. (2020). Optimisation of physical and chemical treatments to control browning development and enzymatic activity on fresh-cut apple slices. Foods, 9(1), 76. https://doi.org/10.3390%2Ffoods9010076.
Silva, J.D.F.; Correa, A.P.F ; Kechinski,C.P. and Brandell,A. 2018. Buffalo cheese whey hydrolyzed with Alcalase as an anti-browning agent in minimally processed apple. J Food Sci Technol, 55(9):3731–3738. https://doi.org/10.1007/s13197-018-3303-y.
Singh, B., Suri, K., Shevkani, K., Kaur, A., Kaur, A., & Singh, N. 2018. Enzymatic browning of fruit and vegetables: A review. Enzymes in food technology: Improvements and innovations, 63-78. https://doi.org/10.1007/978-981-13-1933-4_4.
Singh, G. 2016. Manual of Methods of Analysis of Foods (Meat and Meat Products & Fish and Fish Products). Food Safety and Standards Authority of India, Ministry of Health and Family Welfare, Government of India New Delhi.p:88.
Starowicz, M., Achrem–Achremowicz, B., Piskuła, M. K., Zieliński, H. 2020. Phenolic compounds from apples: reviewing their occurrence, absorption, bioavailability, processing, and antioxidant activity–a review. Polish Journal of Food and Nutrition Sciences, 70(4), 321-336. https://doi.org/10.31883/pjfns/127635.
Sun,C.; Shan,Y.; Tang, X. ; Han,D.; Wu,X.; Wu,H.and Hosseininezhad,M. 2021. Effects of enzymatic hydrolysis on physicochemical property and antioxidant activity of mulberry (Morus atropurpurea Roxb.) leaf protein. Food Sci Nutr. :9:5379–5390. https://doi.org/10.1002/fsn3.2474.
Supapvanich, S.; Pimsaga, J. and Srisujan, P. 2011. Physiochemical changes in fresh-cut wax apple (Syzygium samarangenese Blume Merrill and L.M. Perry) during storage. Food Chemistry 127: 912-917. https://doi.org/10.1016/j.foodchem.2011.01.058.
Zamora-Sillero, J., Gharsallaoui, A., & Prentice, C. 2018. Peptides from fish by-product protein hydrolysates and its functional properties: An overview. Marine Biotechnology, 20, 118-130. https://doi.org/10.1007/s10126-018-9799-3.
Zhong, J., Lu, P., Wu, H., Liu, Z., Sharifi-Rad, J., Setzer, W. N., Suleria, H. A. 2022. Current insights into phytochemistry, nutritional, and pharmacological properties of Prosopis plants. Evidence-Based Complementary and Alternative Medicine, Vol: 2022, Article ID 2218029, 18 pages. https://doi.org/10.1155/2022/2218029.