تثبیت کووالانت بتا گالاکتوزیداز آسپرژیلوس اوریزه و پروتئاز باسیلوس لیکنی فورمیس بر آمینو- نانولوله های کربنی چند دیواره ای

نویسندگان
آلان یاسین طاهر 1
محمد علیزاده خالدآباد 2
یعقوب اصلان 3
1 داﻧﺸﺠﻮی دکتری ﻋﻠﻮم و ﺻﻨﺎیﻊ غذایی، گروه ﻋﻠﻮم و مهندسی ﺻﻨﺎیﻊ غذایی، دانشکده کشاورزی ، دانشگاه اروﻣﯿﻪ، اروﻣﯿﻪ، ایﺮان.
2 اﺳﺘﺎد گروه ﻋﻠﻮم و مهندسی ﺻﻨﺎیﻊ غذایی ، دانشکده کشاورزی، دانشگاه اروﻣﯿﻪ، اروﻣﯿﻪ، ایﺮان
3 استاد گروه علوم و مهندسی صنایع غذایی، دانشکده فنی مهندسی، دانشگاه سیرت، ترکیه
10.22034/FSCT.20.145.208
چکیده
این مطالعه با هدف تثبیت کووالانت بتا گالاکتوزیداز آسپرژیلوس اوریزه و پروتئاز باسیلوس لیکنی فورمیس برروی آمینو- نانولوله­های کربنی چند دیواره­ای انجام شد. در این روش ازطرح 2k کسری برای مطالعه اثر هفت فاکتور پیوسته (pH فعال سازی، مولاریته گلوتارآلدئید، زمان فعال سازی، pHمحلول بافر، مولاریته محلول بافر، مقدار MWCNT-NH3-گلوتارآلدئید و زمان تثبیت) بر روی راندمان تثبیت و فعالیت آنزیمی استفاده شد. از نرم افزارDesign-expert برای آنالیز داده ها و رسم نمودار ها استفاده شد. نتایج نشان داد که فاکتور های مذکور میزان فعالیت آنزیمی پروتئاز باسیلوس لیکنی فورمیس و بتا گالاکتوزیداز آسپرژیلوس اوریزه به ترتیب با ضریب همبستگی 80/0 و 92/0 به میزان 77 و 88 درصد پیش بینی می کنند. همچنین ضریب همبستگی مدل راندمان تثبیت کووالانت بتا گالاکتوزیداز آسپرژیلوس اوریزه و پروتئاز باسیلوس لیکنی فورمیس برروی آمینو- نانولوله­های کربنی چند دیواره­ای به ترتیب 89/0 و 82/0 بدست آمد و فاکتورهای مورد مطالعه توانستند به ترتیب میزان راندمان تثبیت کووالانت بتا گالاکتوزیداز آسپرژیلوس اوریزه و پروتئاز باسیلوس لیکنی فورمیس برروی آمینو- نانولوله­های کربنی چند دیواره­ای به ترتیب 83 و 77 درصد پیش بینی کنند.
کلیدواژه‌ها
پروتئاز قلیایی
نانو لوله های‌ کربن
تثبیت کووالانس
بتا گالاکتوزیداز

موضوعات

عنوان مقاله English

Covalent immobilization of Aspergillus oryzae β-galactosidase and Bacillus licheniformis protease with Amino-Multi Walled Carbon Nanotubes

نویسندگان English

Alan Yaseen Taher 1
Mohammad Alizadeh Khaledabad 2
Yakup Aslan 3
1 Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
3 Siirt University Faculty of Engineering Department of Food Engineering
چکیده English

This study was carried out with the aim of covalent immobilization of Aspergillus oryzae beta-galactosidase and Bacillus licheniformis protease on multi-walled amino-carbon nanotubes. In this method, fractional 2k design was used to study the effect of seven continuous factors (activation pH, glutaraldehyde molarity, activation time, buffer solution pH, buffer solution molarity, MWCNT-NH3-glutaraldehyde amount and stabilization time) on the stabilization efficiency and enzyme activity. . Design-expert software was used to analyze data and draw graphs. The results showed that the aforementioned factors predict the level of enzyme activity of Bacillus licheniformis protease and Aspergillus oryzae beta-galactosidase with correlation coefficients of 0.80 and 0.92 at the rate of 77 and 88%, respectively. Also, the correlation coefficient of the covalent fixation efficiency model of Aspergillus oryzae beta-galactosidase and Bacillus licheniformis protease on multi-walled carbon nanotubes was 0.89 and 0.82, respectively, and the studied factors were able to determine the covalent fixation beta efficiency, respectively. Aspergillus oryzae galactosidase and Bacillus licheniformis protease on multi-walled amino-carbon nanotubes predict 83 and 77%, respectively.

کلیدواژه‌ها English

Alkaline Protease
Carbon nanotubes
covalent immobilization
β-galactosidase
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