کاربرد روش رسوب ضدحلال در ریزپوشانی عصاره‌های هسته رقم خرمای ربی در بیوپلیمر پروتئینی زئین

نویسندگان
عبدالواحد صفرزائی 1
رضا اسماعیل زاده کناری 1
رضا فرهمندفر 1
احمد مهرآوران 2
حامد فنایی 3
1 گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران
2 گروه انگل و قارچ شناسی، دانشگاه علوم پزشکی زاهدان، ایران
3 گروه فیزیولوژی، دانشگاه علوم پزشکی زاهدان، ایران
10.22034/FSCT.20.145.188
چکیده
هدف از این مطالعه، بررسی خصوصیات آنتی ­اکسیدانی عصاره­ های متانولی استخراجی از هسته رقم خرمای ربی به روش فراصوت و ارزیابی ویژگی­ های فیزیکوشیمیایی نانوذرات زئین بارگذاری شده با این عصاره ­ها بود. تیمار فراصوت جهت استخراج عصاره متانولی از پودر هسته خرما و روش رسوب ضدحلال جهت ریزپوشانی عصاره­ های متانولی هسته خرما به میزان 05/0، 1/0، 15/0 و 2/0 گرم در حامل بیوپلیمری زئین مورد استفاده قرار گرفت. در این مطالعه، میزان ترکیبات فنولی کل و IC50 عصاره­ های استخراجی از هسته خرمای رقم ربی در زمان 45 دقیقه، دمای50 درجه سانتیگراد حمام فراصوت و غلظت حلال متانول 70 درصد به ترتیب 74/369 میلی­ گرم اکی ­والان اسید گالیک بر گرم وزن خشک و 08/5 میکروگرم بر میلی­ لیتر بدست آمد. با افزایش نسبت عصاره ریزپوشانی شده در حامل زئین از 1/0 تا 4/0، میزان کارایی ریزپوشانی از 90/85 به 19/95درصد و اندازه ذرات زئین بارگذاری شده با عصاره­های متانولی از 95/129 به 30/183 نانومتر افزایش و میزان پتانسیل زتا نانوذرات از 15/19+ به 48/14+ میلی­ولت کاهش یافت. میزان اندازه و پتانسیل زتا ذرات زئین فاقد عصاره به ترتیب 30/109 نانومتر و 96/21+ میلی ­ولت تعیین گردید. نتایج آنالیز ATR-FTIR بیانگر آن بود که با افزایش نسبت عصاره متانولی ریزپوشانی شده در حامل زئین، پیک کشش پیوند O‒H⋯O از 85/3292 به 85/3294 cm-1 تغییر و افزایش یافته است. در بررسی تصاویر FE-SEM، نانوذرات زئین فاقد عصاره و بارگذاری شده با عصاره ­های هسته خرما دارای مرفولوژی نیمه کروی بودند. به طور کلی، ماهیت آبگریز حامل زئین باعث اتصال آن با ترکیبات فنولی عصاره هسته خرمای ربی از طریق ایجاد اینتراکشن­های غیرکوالانس، واندروالس، هیدروژنی و هیدروفوب گردیده است. از این رو، زئین می­ تواند به عنوان یک حامل قدرتمند در ریزپوشانی عصاره­ های هسته خرما مورد استفاده قرار گیرد.
کلیدواژه‌ها
فراصوت
هسته خرمای ربی
حامل زئین
نانوریزپوشانی
رسوب ضدحلال

موضوعات

عنوان مقاله English

Application of antisolvent precipitation method for encapsulation of date seed extracts of Rabbi variety in zein ptotein biopolymer

نویسندگان English

Abdolvahed Safarzaei 1
Reza Esmaeilzadeh kenari 1
Reza Farahmandfar 1
Ahmad Mehravaran 2
Hamed Fanaei 3
1 Department of Food Science and Technology, Sari Agricultural Sciences and Natural Resources University, Iran
2 Department of Parasitology and Mycology, School of Medicine, Zahedan University of Medical Sciences, Iran
3 Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Iran
چکیده English

The aim of this study was to investigate the antioxidant properties of date seed methanolic extracts of Rabbi variety and evaluating the physicochemical properties of zein nanoparticles loaded with these extracts. Ultrasonic treatment was used to extract methanolic extract from date seed powder and antisolvent precipitation method was used to encapsulate date seed methanolic extracts in amounts of 0.05, 0.1, 0.15 and 0.2 g in zein biopolymer carrier. In this study, the total phenolic content (TPC) and half maximal inhibitory concentration (IC50) value of date seed extracts of Rabbi variety in 45 min, the temperature of 500C of ultrasonic bath and 70% methanol solvent concentration were obtained respectively 369.47 mg gallic acid equivalents (GAE)/g dry weight and 5.08 µg/m1. By increasing the ratio of encapsulated extract in zein carrier from 0.1 to 0.4, the encapsulation efficiency from 85.90 to 95.19% and the size of zein particles loaded with methanolic extracts from 129.95 to 183.30 nm increased and the zeta potential of nanoparticles decreased from +19.15 to +14.48 mV. The size and zeta potential of extract-free zein particles were determined 109.30 nm and +21.96 mV, respectively. The results of ATR-FTIR analysis indicated that with increase the ratio of methanolic extract encapsulated in the zein carrier, the stretching peak of O‒H⋯O bond changed and increased from 3292.85 to 3294.85 cm-1. In Investigating the FE-SEM images, extract-free zein nanoparticles and zein nanoparticles loaded with date seed extracts had semi-spherical morphology. Overall, the hydrophobic nature of zein carrier caused it to bind with the phenolics of Rabbi date seed extract through non-covalent, van der Waals, hydrogen, and hydrophobic interactions. Therefore, it can be used as a strong carrier for the encapsulation of date seed extracts.


کلیدواژه‌ها English

Ultrasound
Rabbi date seed
Zein carrier
Nanoencapsulation
Antisolvent precipitation
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