ارزیابی ویژگی‌های آنتی‌اکسیدانی و ضدمیکروبی عصاره‌های اتانولی استخراجی از میوه گیاه کمرگل به روش فراصوت با استفاده از مدل سطح پاسخ

نویسندگان
عبدالواحد صفرزایی 1
رضا اسماعیل زاده کناری 1
رضا فرهمندفر 1
علیرضا خلیقی مهر 2
1 دانشگاه علوم کشاورزی و منابع طبیعی ساری
2 دانشگاه تهران
10.22034/FSCT.20.145.1
چکیده
ویژگی­های بالقوه آنتی­اکسیدانی و ضدمیکروبی چشمگیر می­باشند. انتخاب روش استخراج مناسب بر کمیت و کیفیت ترکیبات آنتی­اکسیدانی و ضدمیکروبی اثرگذار است. هدف از این مطالعه، بررسی کارایی امواج فراصوت در استخراج ترکیبات فنلی، آنتی‌اکسیدانی و ضدمیکروبی از میوه گیاه کمرگل بود. بهینه‌سازی با استفاده از روش آماری سطح پاسخ و طرح باکس بنکن با انتخاب فاکتور زمان فراصوت­دهی در سه سطح (10، 25 و 40 دقیقه) و شدت امواج فراصوت در سه سطح (40، 70 و 100 درصد) و کاربرد حلال اتانول 70 درصد انجام گردید. از نتایج آزمون‌های انجام شده با روش آماری سطح پاسخ، شدت فراصوت به عنوان موثرترین فاکتور استخراج ترکیبات فنلی، آنتی‌اکسیدانی و ضدمیکروبی از میوه گیاه کمرگل شناخته شد و با افزایش زمان و شدت امواج فراصوت میزان استخراج این ترکیبات افزایش یافت. شرایط بهینه استخراج ترکیبات آنتی‌اکسیدانی و ضدمیکروبی از میوه گیاه کمرگل، زمان 36 دقیقه و شدت فراصوت 91 درصد تعیین گردید و در این شرایط بهینه، میزان ترکیبات فنلی کل و شاخص IC50 عصاره­های اتانولی استخراجی از میوه گیاه به ترتیب 63/23 میلی­گرم بر گرم و 30/45 میکروگرم بر میلی‌گرم، میزان بهینه حداقل غلظت بازدارندگی از رشد باکتری­های استافیلوکوکوس اورئوس کوآگولاز مثبت و باسیلوس سرئوس به ترتیب 19/0 و 07/6 میلی‌گرم بر میلی‌لیتر و میزان بهینه حداقل غلظت باکتری­کشی استافیلوکوکوس اورئوس کوآگولاز مثبت 81/11 میلی‌گرم بر میلی‌لیتر حاصل گردید. همچنین عصاره­های استخراجی در شدت فراصوت 91 درصد و زمان 36 دقیقه دارای قابلیت مهار فعالیت باکتری­های اشریشیاکلی: H7 O157 و سودوموناس آئروژینوزا در غلظت­های به ترتیب 25 و 25 میلی‌گرم بر میلی‌لیتر و قابلیت کشندگی باکتری­های باسیلوس سرئوس، اشریشیاکلی: H7 O157 و سودوموناس آئروژینوزا در غلظت­های به ترتیب 25، 50 و 50 میلی‌گرم بر میلی‌لیتر بودند.
کلیدواژه‌ها
گیاه کمرگل
آنتی‌اکسیدانی
ضدمیکروبی
حمام فراصوت

موضوعات

عنوان مقاله English

Evaluation of antioxidant and antimicrobial properties of ethanolic extracts extracted from Caper (Capparis spinosa) fruit by ultrasound method using the RSM

نویسندگان English

Abdolvahed Safarzaei1 Abdolvahed Safarzaei 1
reza esmaeilzadeh kenari 1
Reza Farahmandfar 1
alireza khalighimehr 2
1 Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Tehran University
چکیده English

Extracts and essential oils of medicinal plants have many bioactive agents, including phenolic compounds with significant antioxidant and antimicrobial properties. Choosing the suitable extraction method affects the quantity and quality of antioxidant and antimicrobial compounds. The aim of this study was to evaluate the efficiency of ultrasound waves in extracting phenolic, antioxidant and antimicrobial compounds of Caper fruit. Response surface methodology (RSM) and Box–Behnken design were used to optimize the extraction factors, including extraction time (10, 25, 40 min) and ultrasound intensity (40, 70, 100 %) with ethanolic solvent (70 %). From the results of the tests performed with the response surface methodology, ultrasound intensity was recognized as the most effective factor in extracting phenolic, antioxidant and antimicrobial compounds of Caper fruit and by increasing time and ultrasound intensity, the extraction of these compounds increased. The optimum conditions for extraction of antioxidant and antimicrobial compounds of Caper fruit were determined extraction time 36 minutes and ultrasound intensity 91 percent. In these optimum conditions, the amount of total phenolic contents and IC50 index of ethanolic extracts extracted from Caper fruit were obtained 23.63 mg/g and 45.30 μg/mg, respectively, the optimum amount of minimum inhibitory concentration of coagulase positive Staphylococcus aureus and Bacillus cereus were obtained 0.19 and 6.07 mg/ml, respectively, and the optimum amount of minimum batericidal concentration of coagulase positive Staphylococcus aureus was obtained 11.81 mg/ml. Also, the extracts extracted at time 36 minutes and ultrasound intensity 91 percent had the ability to inhibit the activity of Escherichia coli O157: H7 and Pseudomonas aeruginosa at concentrations of 25 and 25 mg/ml, respectively, and had the batericidal ability of Bacillus cereus, Escherichia coli O157: H7 and Pseudomonas aeruginosa at concentrations of 25, 50 and 50 mg/ml, respectively.

کلیدواژه‌ها English

Antioxidant
Antimicrobial
Ultrasonic bath
caper
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