جداسازی و شناسایی باکتری های تولید کننده آمیلاز مقاوم به حرارت از فاضلاب کارخانه کنسروسازی

نویسندگان
سوده یوسفی فخر 1
نفیسه دعوتی 2
داریوش مینایی تهرانی 3
1 دانشجوی کارشناسی ارشد، گروه زیست شناسی سلولی – مولکولی، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران
2 استادیار، گروه علوم و صنایع غذایی، دانشکده صنایع غذایی بهار، دانشگاه بوعلی سینا، همدان، ایران
3 دانشیار، گروه زیست شناسی سلولی - مولکولی، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران
10.22034/FSCT.20.139.138
چکیده
هدف این مطالعه جداسازی سویه­های میکروبی مولد آمیلاز مقاوم به حرارت از فاضلاب غنی از نشاسته یکی از کارخانه های کنسروسازی و سپس شناسایی مولکولی این جدایه­ها بود. به­علاوه آمیلاز مقاوم به حرارت استخراج شده از باکتری به لحاظ دما و pH بهینه فعالیت آنزیمی مورد بررسی قرار گرفت. در این مطالعه 14 جدایه میکروبی مقاوم به حرارت از پساب جدا گردید، که تنها دو جدایه دارای فعالیت آمیلازی بود. شناسایی مولکولی این دو جدایه براساس تکثیر ژن 16S rDNA توسط پرایمرهای B27F و U1492R و سپس توالی­یابی محصول PCR ، حضور Bacillus pumilus وBacillus safensis را تایید کرد. نتایج نشان داد که دما و pH بهینه فعالیت آمیلازی جدایه ­ها به ترتیب60 درجه سانتی­گراد و 7 بود و با عملکرد Bacillus safensis (U/ml) 7.67 دارای فعالیت آمیلاز بیشتری نسبت به Bacillus pumilus با عملکرد (U/ml) 6.33 بود.
کلیدواژه‌ها
آمیلاز مقاوم به حرارت
باسیلوس
دما
پساب کارخانه

موضوعات

عنوان مقاله English

Isolation and identification of thermostable amylase producing bacteria from the wastewater of the canning factory

نویسندگان English

Soudeh Yousefi fakhr 1
nafiseh davati 2
Dariush Minai-Tehrani3 3
1 MSc Student, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2 Assistant professor, Department of Food Science and Technology, Faculty of Food Industry, Bu-Ali SinaUniversity, Hamedan, Iran.
3 Associate professor, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
چکیده English

The aim of this study was to isolate thermostable amylase producing bacteria from starch-rich wastewater of one of ­the canning factories and then molecular identification of these isolates. In addition, the thermostable amylase extracted from the bacterium was investigated for the optimum temperature and pH of enzyme activity. In this study, 14 heat-resistant microbial isolates were isolated from wastewater and only two isolates had amylase activity. Molecular identification of isolates based on amplification of 16S rDNA gene by B27F and U1492R primers and then sequencing of PCR product confirmed the presence of Bacillus pumilus and Bacillus safensis. The results showed that the optimum temperature and pH of amylase activity were 60°C and 7, ­respectively, and Bacillus safensis 7.67 (U/ml) had more amylase activity than of Bacillus pumilus 6.33 (U/ml).

کلیدواژه‌ها English

Thermostable amylase
Bacillus
Temperature
Factory wastewater
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