تولید بیوپلیمر زیست‌تخریب‌پذیر بر پایه کیتوزان- نشاسته سیب زمینی حاوی عصاره دانه کرفس برای بسته‌بندی فیله مرغ

نویسندگان
1 علوم و صنایع غذایی، موسسه آموزش عالی خزر محمودآباد، ایران
2 بخش تحقیقات بیوتکنولوژی، موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران
3 دانشیار گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
چکیده
تمایل مردم به استفاده از نگهدارنده­های طبیعی در فرآورده­های غذایی به جای مواد سنتزی و ایجاد فرهنگ استفاده نکردن از مواد پلاستیکی رو به افزایش است. در این تحقیق، فیلم کیتوزان- نشاسته سیب زمینی حاوی عصاره دانه کرفس در سطوح غلظتی مختلف (5/0، 2 و 8 درصد) تهیه و خصوصیات فیزیکی فیلم از جمله ضخامت، میزان رطوبت و قابلیت انحلال آن، خصوصیات مکانیکی و آنتی اکسیدانی تعیین ­شد. سپس فیله مرغ پوشش دهی شده با فیلم مورد نظر در مدت 15 روز در دمای یخچال نگهداری و میزان رشد میکروبی و تولید ترکیبات اولیه و ثانویه اکسایش با شاخص پراکسید و اسید تیوباربیتوریک و همچنین ارزیابی حسی طی زمان مورد پایش قرار گرفت. نتایج حاصل نشان داد که محتوی فنل و فلاونوئید کل عصاره دانه کرفس به ترتیب 8/7±67/70 میلی گرم اسید گالیک بر گرم عصاره و 65/5 ±81/62 میلی­گرم کوئرستین در هر گرم از عصاره بود. افزودن عصاره دانه کرفس (2 و 8 درصد) میزان رطوبت و حلالیت فیلم را کاهش داد. علت آن افزایش خاصیت آب­گریزی فیلم بود. همچنین با افزودن عصاره دانه کرفس، میزان افزایش طول تا پارگی فیلم افزایش در حالی که مقاومت در برابر کشش و مدول الاستیک آن­ کاهش یافت. با افزایش غلظت عصاره دانه کرفس در فیلم، میزان مهار رادیکال آزاد DPPH از 69/14 به 94/73 درصد افزایش می­یابد. براساس آزمون اکسایشی، میکروبی و ارزیابی حسی، در صورت پوشش­دهی شده با فیلم کیتوزان-نشاسته سیب زمینی حاوی 8 درصد عصاره دانه کرفس می­توان زمان ماندگاری فیله مرغ را تا 15 روز در یخچال افزایش داد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Biodegradable packaging based on chitosan-potato starch biopolymer containing Apium graveolens seed extract for chicken fillets

نویسندگان English

Farzaneh Karamali 1
Maryam Asnaashari 2
Reza Farahmandfara 3
1 Department of Food Science and Technology, Khazar Institute of Higher Education, Mahmoudabad, Iran
2 Department of Biotechnology, Animal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 Associate Professor, Department of Food Science and Technology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
چکیده English

The consumer demand to use natural preservatives in food products instead of synthetic ones and awareness about not using plastic is increasing. In this research, chitosan-potato starch film containing celery seed extract was prepared at different concentration levels (0.5, 2 and 8%) and the physical properties of the film (thickness, moisture content and solubility), mechanical properties and antioxidant activity also determined. Then, the chicken fillet wrapped by the film was kept in the refrigerator for 15 days and the microbial growth and primary and secondary oxidation compounds formation with peroxide value and thiobarbituric acid and sensory evaluation was also monitored during the storage time. The results showed that the total phenol and flavonoid content of celery seed extract was 70.67±7.8 mgGA/g of extract and 62.81±5.65 mg Quercetin/g of extract, respectively. Addition of celery seed extract (2 and 8%) decreased the moisture content and solubility of the film due to the increase of the film hydrophobicity. Also, by adding the celey seed extract, the elongation at break of the film increased, while the tensile strength and elastic modulus decreased. With the increase in the concentration of the celery seed extract in the film, the DPPH free radical inhibition increases from 14.69 to 73.94%. According to the results of microbial and oxidation tests as well as sensory evaluation, coating a chicken fillet with a chitosan-potato starch film containing 8% celery seed extract would increase its shelf life by up to 15 days.

کلیدواژه‌ها English

packaging
Natural biopolymer
Celery seed
Chitosan
chicken fillet
Zhao, Y., et al., Development of antimicrobial films based on cassava starch, chitosan and gallic acid using subcritical water technology. The Journal of Supercritical Fluids, 2018. 137: p. 101-110.
2. Pineros-Hernandez, D., et al., Edible cassava starch films carrying rosemary antioxidant extracts for potential use as active food packaging. Food Hydrocolloids, 2017. 63: p. 488-495.
3. Hauser, C., et al., Development of an Active Packaging Film Based on a Methylcellulose Coating Containing Murta (Ugni molinae Turcz) Leaf Extract. Food and bioprocess technology, 2016. 9(2): p. 298-307.
4. Medina-Jaramillo, C., et al., Active and smart biodegradable packaging based on starch and natural extracts. Carbohydrate polymers, 2017. 176: p. 187-194.
5. Barbosa-Pereira, L., et al., Development of new active packaging films coated with natural phenolic compounds to improve the oxidative stability of beef. Meat science, 2014. 97(2): p. 249-254.
6. Talón, E., et al., Antioxidant edible films based on chitosan and starch containing polyphenols from thyme extracts. Carbohydrate polymers, 2017. 157: p. 1153-1161.
7. Turhan, K.N. and F. Şahbaz, Water vapor permeability, tensile properties and solubility of methylcellulose-based edible films. Journal of Food Engineering, 2004. 61(3): p. 459-466.
8. Rodríguez, M., et al., Combined effect of plasticizers and surfactants on the physical properties of starch based edible films. Food Research International, 2006. 39(8): p. 840-846.
9. Ahmadi, R., et al., Development and characterization of a novel biodegradable edible film obtained from psyllium seed (Plantago ovata Forsk). Journal of Food Engineering, 2012. 109(4): p. 745-751.
10. Pelissari, F.M., et al., Antimicrobial, mechanical, and barrier properties of cassava starch− chitosan films incorporated with oregano essential oil. Journal of agricultural and food chemistry, 2009. 57(16): p. 7499-7504.
11. Vásconez, M.B., et al., Antimicrobial activity and physical properties of chitosan–tapioca starch based edible films and coatings. Food Research International, 2009. 42(7): p. 762-769.
12. Perez, C.D., et al., Development of a high methoxyl pectin edible film for retention of l-(+)-ascorbic acid. Journal of agricultural and food chemistry, 2009. 57(15): p. 6844-6855.
13. Sánchez-González, L., et al., Characterization of edible films based on hydroxypropylmethylcellulose and tea tree essential oil. Food Hydrocolloids, 2009. 23(8): p. 2102-2109.
14. Ghasemlou, M., F. Khodaiyan, and A. Oromiehie, Physical, mechanical, barrier, and thermal properties of polyol-plasticized biodegradable edible film made from kefiran. Carbohydrate Polymers, 2011. 84(1): p. 477-483.
15. Shojaee-Aliabadi, S., et al., Characterization of antioxidant-antimicrobial κ-carrageenan films containing Satureja hortensis essential oil. International journal of biological macromolecules, 2013. 52: p. 116-124.
16. Jouki, M., et al., Effect of glycerol concentration on edible film production from cress seed carbohydrate gum. Carbohydrate polymers, 2013. 96(1): p. 39-46.
17. Khazaei, N., et al., Characterization of new biodegradable edible film made from basil seed (Ocimum basilicum L.) gum. Carbohydrate Polymers, 2014. 102: p. 199-206.
18. Kooti, W., et al., A review on medicinal plant of Apium graveolens. Advanced Herbal Medicine, 2015. 1(1): p. 48-59.
19. Urabe, A.A. and W.J. Aziz, Biosynthesis of cobalt oxide (Co3O4) nanoparticles using plant extract of Camellia sinensis (L.) Kuntze and Apium graveolens L. as the antibacterial application. World News of Natural Sciences, 2019. 24: p. 357-365.
20. Jerman, T., P. Trebše, and B.M. Vodopivec, Ultrasound-assisted solid liquid extraction (USLE) of olive fruit (Olea europaea) phenolic compounds. Food Chemistry, 2010. 123(1): p. 175-182.
21. Muntana, N. and S. Prasong, Study on total phenolic contents and their antioxidant activities of Thai white, red and black rice bran extracts. Pakistan Journal of Biological Sciences: PJBS, 2010. 13(4): p. 170-174.
22. Shen, Y., et al., Total phenolics, flavonoids, antioxidant capacity in rice grain and their relations to grain color, size and weight. Journal of Cereal Science, 2009. 49(1): p. 106-111.
23. Silva-Pereira, M.C., et al., Chitosan/corn starch blend films with extract from Brassica oleraceae (red cabbage) as a visual indicator of fish deterioration. LWT-Food Science and Technology, 2015. 61(1): p. 258-262.
24. Ojagh, S.M., et al., Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water. Food Chemistry, 2010. 122(1): p. 161-166.
25. Hosseini, M., S. Razavi, and M. Mousavi, Antimicrobial, physical and mechanical properties of chitosan‐based films incorporated with thyme, clove and cinnamon essential oils. Journal of food processing and preservation, 2009. 33(6): p. 727-743.
26. Sánchez-González, L., J.I.Q. Saavedra, and A. Chiralt, Antilisterial and physical properties of biopolymer films containing lactic acid bacteria. Food control, 2014. 35(1): p. 200-206.
27. Moradi, M., et al., Characterization of antioxidant chitosan film incorporated with Zataria multiflora Boiss essential oil and grape seed extract. LWT-Food Science and Technology, 2012. 46(2): p. 477-484.
28. Sogut, E. and A.C. Seydim, The effects of Chitosan and grape seed extract-based edible films on the quality of vacuum packaged chicken breast fillets. Food Packaging and Shelf Life, 2018. 18: p. 13-20.
29. Serrano-León, J.S., et al., Chitosan active films containing agro-industrial residue extracts for shelf life extension of chicken restructured product. Food Research International, 2018. 108: p. 93-100.
30. Sharafati Chaleshtori, F., et al., Effect of chitosan incorporated with cumin and eucalyptus essential oils as antimicrobial agents on fresh chicken meat. Journal of food processing and preservation, 2016. 40(3): p. 396-404.
31. Adebo, O.A. and I. Gabriela Medina-Meza, Impact of fermentation on the phenolic compounds and antioxidant activity of whole cereal grains: A mini review. Molecules, 2020. 25(4): p. 927.
32. Afshari, K., et al., Natural flavonoids for the prevention of colon cancer: A comprehensive review of preclinical and clinical studies. Journal of Cellular Physiology, 2019. 234(12): p. 21519-21546.
33. Dellal, A., et al., Optimization of the extraction conditions of phenolic compounds from (Apium Graveolens) seeds by response surface methodology. Advances in Environmental Biology, 2016. 10(10): p. 155-164.
34. Mahdizade, T., Tajik, H., Razavi rouhani, S.M., Oromiehie A R. Antibacterial, antioxidant and optical properties of edible starch-chitosan composite film containing pomegranate peel extract. Studies in Medical Sciences 2012; 23 (3) :315-323 (In Persian)
35. Hoque, M.S., S. Benjakul, and T. Prodpran, Properties of film from cuttlefish (Sepia pharaonis) skin gelatin incorporated with cinnamon, clove and star anise extracts. Food Hydrocolloids, 2011. 25(5): p. 1085-1097.
36. Zinoviadou, K.G., K.P. Koutsoumanis, and C.G. Biliaderis, Physico-chemical properties of whey protein isolate films containing oregano oil and their antimicrobial action against spoilage flora of fresh beef. Meat Science, 2009. 82(3): p. 338-345.
37. Mahdavi, V., S.E. Hosseini, and A. Sharifan, Effect of edible chitosan film enriched with anise (Pimpinella anisum L.) essential oil on shelf life and quality of the chicken burger. Food science & nutrition, 2018. 6(2): p. 269-279.
38. Jutaporn, C., C. Suphitchaya, and W. Thawien, Antimicrobial activity and characteristics of edible films incorporated with Phayom wood (Shorea tolura) extract. International Food Research Journal, 2011. 18(1).
39. Pranoto, Y., V.M. Salokhe, and S.K. Rakshit, Physical and antibacte rial properties of alginate-based edible film incorporated with garlic oil. Food research international, 2005. 38(3): p. 267-272.
40. López-Caballero, M., et al., A chitosan–gelatin blend as a coating for fish patties. Food hydrocolloids, 2005. 19(2): p. 303-311.
41. Pires, C., et al., Characterization of biodegradable films prepared with hake proteins and thyme oil. Journal of Food Engineering, 2011. 105(3): p. 422-428.
42. Molaee Aghaee, E., Kamkar, A., Akhondzadeh Basti, A., Khanjari, A., Kontominas, M. Effect of packaging with Chitosan biodegradable films formulated with Garlic essential oil (Allium sativum L.) on the chemical properties of chicken fillet. Iranian Journal of Health and Invironment, 2015; 8 (3) :379-390 (In Persian).
43. Vital, A.C.P., et al., Effect of edible and active coating (with rosemary and oregano essential oils) on beef characteristics and consumer acceptability. PloS one, 2016. 11(8): p. e0160535.
44. Jridi, M., et al., Physical, structural, antioxidant and antimicrobial properties of gelatin–chitosan composite edible films. International journal of biological macromolecules, 2014. 67: p. 373-379.
45. Deus, D., et al., Effect of a nano-silver coating on the quality of fresh turkey meat during storage after modified atmosphere or vacuum packaging. Poultry science, 2017. 96(2): p. 449-457.
46. Gómez-Estaca, J., et al., Antimicrobial activity of composite edible films based on fish gelatin and chitosan incorporated with clove essential oil. Journal of Aquatic Food Product Technology, 2009. 18(1-2): p. 46-52.
47. Ruíz-Cruz, S., et al., Effects of chitosan-tomato plant extract edible coatings on the quality and shelf life of chicken fillets during refrigerated storage. Food Science and Technology, 2018. 39: p. 103-111.
48. Zheng, W.-L., et al., ROS-mediated cell cycle arrest and apoptosis induced by zearalenone in mouse sertoli cells via ER stress and the ATP/AMPK pathway. Toxins, 2018. 10(1): p. 24.
49. Hasanzadeh, P., Tajik, H., Razavi rouhani, S.M., Application of chitosan edible coating containing grape seed extract on the quality and shelf life of refrigerated chicken meat, Journal of Food Research, 1390. 21(4): P. 467-482 (In Persian).
50. Baananou, S., et al., Antiulcerogenic and antibacterial activities of Apium graveolens essential oil and extract. Natural product research, 2013. 27(12): p. 1075-1083.