بررسی اثر لاکتوفرین آزاد و نانولیپوزومی بر بیوفیلم پلی میکروبی مشتق شده از بزاق در مدل Active Attachment biofilm

نویسندگان
پریسا حبیبی 1
فریده طباطبایی یزدی 2
سید علی مرتضوی 2
محمد مراد فرج الهی 3
1 دانشجو دکتری صنایع غذایی دانشگاه فردوسی
2 استاد دانشگاه فردوسی
3 استاد دانشگاه علوم پزشکی ایران
10.22034/FSCT.20.136.26
چکیده
پوسیدگی دندان از مشکلات رایج در دنیا می‌باشد که ناشی از رشد بیوفیلم پوسیدگی‌زا وتولید اسید توسط آنان است. برای حل این مشکل راهکارهای متعددی به کار گرفته شده‌است. اما به دلیل افزایش مقاومت آنتی‌بیوتیکی میکروارگانیسم‌ها و نیاز روز افزون به مواد ضد میکروبی تلاش برای استفاده از مواد مواد ضدمیکروبی طبیعی ادامه دارد. لاکتوفرین یک پروتئین در شیر و بزاق است که خواص ضد میکروبی و ضد بیوفیلمی نشان داده‌است. در این تحقیق ابتدا برای افزایش ویژگی‌ها ضد میکروبی، لاکتوفرین به وسیله نانولیپوزوم ریزپوشانی ‌شد. سپس جهت سنجش تأثیر آن بر تعداد باکتری در بیوفیلم پلی میکروبی و تولید اسید، هریک از مواد آزاد یا نانولیپوزومی در4 غلظت (0, 1.5, 3, 6 میلی گرم بر میلی لیتر) در مدل Active Attachment biofilm به همراه بزاق و محیط کشت گرمخانه گذاری شد. نتایج نشان داد که نانوریزپوشانی لاکتوفرین به دلیل رهایش آهسته لاکتوفرین از لیپوزوم‌ها توانایی مهار بیوفیلم و تولید اسید توسط این باکتری را افزایش داد. تبا افزایش غلظت لاکتوفرین آزاد و نانولیپوزومی تا غلظت 3 میلی گرم بر میلی لیتر کاهش قابل توجهی در تعداد باکتری ها در بیوفیلم نسبت به نمونه شاهد مشاهده شد (01/0< P). اما افزایش بیشتر غلظت لاکتوفرین آزاد موجب افزایش مجدد تعداد باکتری ها در بیوفیلم شد. این درحالیست که لاکتوفرین نانولیپوزومی در غلظت 6 میلی گرم بر میلی لیتر همچنان باعث کاهش باکتری در بیوفیلم شد که این کاهش نسبت به غلظت 3میلی گرم بر میلی لیتر معنی دار نبود (01/0> P). از نتایج بدست آمده می‌توان نتیجه گرفت که از لاکتوفرین نانولیپوزومی می توان جهت طراحی محصولات مرتبط با سلامت دهان و دندان استفاده کرد.
کلیدواژه‌ها
لاکتوفرین
نانولیپوزوم
بیوفیلم پلی میکروبی
پوسیدگی دندان

موضوعات

عنوان مقاله English

Investigating the effect of free and nanoliposomal lactoferrin on polymicrobial biofilms derived from saliva in an Active Attachment biofilm model

نویسندگان English

Parisa Habibi 1
farideh tabatabaei yazdi 2
Seyyed Ali Mortazavi 2
Mohammad Morad Farajollahi 3
1 Ph.D. student of Ferdowsi University
2 Professor of Ferdowsi university
3 Professor of Iran University of Medical Sciences
چکیده English

Tooth decay is one of the most common problems in the world, which is caused by the growth of biofilm and acid production by them. Many solutions have been used to solve this problem. However, due to the increase in antibiotic resistance of microorganisms and the increasing need for antimicrobial substances, efforts are being made to use natural antimicrobial substances. Lactoferrin is a protein in milk and saliva with antimicrobial and anti-biofilm properties. In this research, lactoferrin was encapsulated by nanoliposomes to increase its antimicrobial properties. In order to measure the effect of lactoferrin on the number of bacteria in the polymicrobial biofilm and acid production, each of the free substances or nanoliposomes in 4 concentrations (0, 1.5, 3, 6 mg/ml) in the Active Attachment biofilm model with saliva and culture medium was incubated.

The results showed that lactoferrin nanocoating increased the ability to inhibit biofilm and acid production by this bacterium due to the slow release of lactoferrin from liposomes. When increasing the concentration of free and nanoliposomal lactoferrin to a concentration of 3 mg/ml, a significant decrease in the number of bacteria in the biofilm was observed compared to the control sample (P<0.01). However, increasing the concentration of free lactoferrin again increased the number of bacteria in the biofilm. Meanwhile, nanoliposomal lactoferrin at a concentration of 6 mg/ml still caused a decrease in bacteria in the biofilm, which was insignificant compared to the concentration of 3 mg/ml (P>0.01). From the obtained results, it can be concluded that nanoliposomal lactoferrin can be used to design products related to oral and dental health.

کلیدواژه‌ها English

Lactoferrin
Nanoliposome
Polymicrobial biofilm
Tooth decay
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