درون پوشانی لاکتوباسیلوس اسیدوفیلوس PTCC 1643 با دیواره کامپوزیتی صمغ زانتان، ایزوله پروتئین سویا و فروکتوالیگوساکارید: بررسی ویژگی‌های میکروکپسول ها

نویسندگان
1 دانشجوی دکترای تخصصی زیست فناوری مواد غذایی، گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران
2 استاد گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران
3 استادیار گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران
چکیده
درون پوشانی روشی موثر برای افزایش زنده مانی باکتری‌های پروبیوتیک می‌باشد. هدف از این پژوهش تعیین فرمولاسیون بهینه برای میکروکپسولاسیون لاکتوباسیلوس اسیدوفیلوس PTCC 1643 با ایزوله پروتئین سویا، صمغ زانتان و فروکتوالیگوساکارید به عنوان مواد دیواره با روش خشک کردن انجمادی می‌باشد. بدین منظور 19 تیمار با طرح Combine توسط نرم افزار Design expert تهیه شدند. آزمون‌های کارایی درون پوشانی، اندازه ذرات، پتانسیل زتا، و زنده مانی پروبیوتیک، FTIR و SEM بر روی نمونه‌ها انجام شد. نمونه بهینه بر اساس کمترین پتانسیل زتا و اندازه ذرات انتخاب گردید و ویژگی­های میکروکپسول، با آزمون­های فوق­الذکر تعیین گردید. نتایج نشان داد زنده مانی پروبیوتیک با افزایش غلظت ایزوله پروتئین سویا و صمغ زانتان به ترتیب افزایش و کاهش یافت. اندازه ذرات نمونه‌ها نیز با افزایش غلظت ایزوله پروتئین سویا افزایش یافت. به طور کلی با توجه به اثر محافظتی صمغ زانتان، ایزوله پروتئین سویا و فروکتوالیگوساکارید بر پروبیوتیک‌ها می‌توان نتیجه گرفت که این ترکیبات به عنوان دیواره برای درون پوشانی می‌توانند برای غنی سازی محصولات غذایی مورد استفاده قرار گیرند.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Encapsulation of Lactobacillus acidophilus PTCC 1643 with composite wall of xanthan gum, soy protein isolate and fructooligosaccharide: investigation microcapsule characteristics

نویسندگان English

Maryam Abbasi Ghaznaq 1
Mahmoud Rezazadeh Bari 2
Mohammad Alizadeh Khaledabad 2
Saber Amiri 3
1 PhD candidate of Food Biotechnology, Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Professor, Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
3 Assistant Professor, Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده English

Encapsulation is an effective method to increase the viability of probiotic bacteria. The aim of this research is to determine the optimal formulation for microencapsulation of Lactobacillus acidophilus PTCC 1643 with soy protein isolate, xanthan gum and fructooligosaccharide as wall materials by freeze drying method. For this purpose, 19 treatments with Combine design were prepared by Design expert software. Encapsulation efficiency, particle size, zeta potential, and probiotic viability, FTIR and SEM tests were performed on the samples. The optimal sample was selected based on the lowest zeta potential and particle size, and the characteristics of microcapsules were determined by the aforementioned tests. The results showed that probiotic viability increased and decreased with increasing concentration of soy protein isolate and xanthan gum, respectively. The particle size of the samples also increased with increasing the concentration of soy protein isolate. In general, according to the protective effect of xanthan gum, soy protein isolate and fructooligosaccharide on probiotics, it can be concluded that these compounds can be used as a wall for coating to enrich food products.

کلیدواژه‌ها English

Soy protein isolate
Fructooligosaccharide
Encapsulation
xanthan gum
Lactobacillus acidophilus PTCC 1643
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