ارزیابی ویژگی‌های پروبیوتیکی سویه‌های تجاری لاکتوباسیلوس پلانتاروم و بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس در شرایط برون‌تنی

نویسندگان
نازیلا دردمه 1
مسعود یاورمنش 1
علی عطا معظمی 2
مریم مقدم متین 3
حمید نوربخش 4
1 گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد
2 گروه علوم مولکولی، دانشگاه علوم کشاورزی سوئد، اوپسالا، سوئد
3 گروه زیست‌شناسی، دانشکده علوم، دانشگاه فردوسی مشهد
4 دکتری تخصصی، گروه زیست‌شناسی سلولی و مولکولی، دانشکده علوم و فناوری‌های زیستی، دانشگاه اصفهان
10.22034/FSCT.19.133.91
چکیده
پروبیوتیک‌ها میکروارگانیسم‌های زنده‌ای هستند که در صورت مصرف به میزان کافی، تأثیرات سودمندی بر سلامت میزبان خواهند داشت. در پژوهش حاضر خصوصیات پروبیوتیکی دو سویه تجاری بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس BB-12 و لاکتوباسیلوس پلانتاروم ATCC 14917 در شرایط برون‌تنی مورد بررسی قرار گرفت. نتایج نشان داد که هر دو سویه مقاومت بالایی در برابر اسید، صفرا و لیزوزیم داشتند. به‌طور کلی میزان زنده‌مانی هر دو سویه در شرایط شبیه‌سازی‌شده معده-روده‌ای بالاتر از 85% بود که امکان زنده‌مانی این دو سویه در دستگاه گوارش را فراهم می‌سازد. به‌علاوه بالاترین میزان آب‌گریزی (75/59%) و خودانبوهش (42/51%) و همچنین کمترین چسبندگی (35/8%) به رده سلولی HT-29 روده‌ی انسان مربوط به بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس BB-12 بود؛ هر دو سویه دارای فعالیت بتا گالاکتوزیدازی بودند و نسبت به آنتی‌بیوتیک‌های پنی‌سیلین، ونکومایسین و تتراسایکلین مقاومت نشان دادند. در پژوهش حاضر مشخص گردید که لاکتوباسیلوس پلانتاروم ATCC 14917نسبت به بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس BB-12 خصوصیات پروبیوتیکی بهتری دارد.
کلیدواژه‌ها
ویژگی‌های پروبیوتیکی
لاکتوباسیلوس پلانتاروم 14917 ATCC
بیفیدوباکتریوم انیمالیس زیرگونه لاکتیس BB-12
دستگاه گوارش

موضوعات

عنوان مقاله English

In vitro evaluation of probiotic properties of commercial strains Lactobacillus plantarum and Bifidobacterium animalis subsp. lactis

نویسندگان English

Nazila Dardmeh 1
masoud yavarmanesh 1
Ali ata Moazzami 2
maryam moghaddam matin 3
Hamid Noorbakhsh 4
1 Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad
2 Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
3 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad,
4 Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan
چکیده English

Probiotics are recognized as live microorganisms that confer a health benefit to the host when administered in adequate amounts. The present study aimed to evaluate in vitro probiotic properties of two commercial probiotic strains, Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus plantarum ATCC 14917. Our results indicated that the selected strains showed high resistance to acid, bile salts, and lysozyme. In general they showed good adaptation to simulated gastric and intestinal juices (more than 85% could survive) which guarantees their survival in the gastrointestinal tract. Moreover, Bifidobacterium animalis subsp. lactis BB-12 showed the highest hydrophobicity (59.75%) and auto-aggregation (51.42%) but the lowest adhesion to the human intestinal HT-29 cell line (8.35%). Furthermore, they both had β-galactosidase activity and were resistant to penicillin, vancomycin, and tetracycline. Our results indicated that Lactobacillus plantarum ATCC 14917 had better characteristics of a probiotic compared to Bifidobacterium animalis subsp. lactis BB-12.

کلیدواژه‌ها English

probiotic properties
Lactobacillus plantarum ATCC 14917
Bifidobacterium animalis subsp. lactis BB-12
gastrointestinal tract
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