بررسی اثر ضد باکتریایی و آنتی اکسیدانی پوشش خوراکی دولایه ژلاتین-کیتوزان حاوی نانوامولسیون اسانس برازمبل روی کنترل رشد باکتری آئروموناس هیدروفیلا تلقیح شده به فیله ماهی قزل آلای رنگین کمان

نویسندگان
کتایون احمدی
محمد محسن زاده
دانشگاه فردوسی مشهد - گروه بهداشت مواد غذایی و آبزیان
10.22034/FSCT.19.133.29
چکیده
در این مطالعه اثر پوشش خوراکی دو لایه ی ژلاتین-کیتوزان حاوی نانوامولسیون اسانس برازمبل بر روی کنترل رشد باکتری آئروموناس هیدروفیلا تلقیح شده به فیله ماهی قزل آلای رنگین کمان طی یک دوره ی 12 روزه در دمای 4 درجه سلسیوس بررسی شده است. ترکیبات شیمیایی اسانس با دستگاه طیف سنج جرمی، خاصیت ضد باکتریایی به روش های انتشار در دیسک، چاهک پلیت و میکرو دایلوشن براث و خاصیت آنتی اکسیدانی با روش احیاء رادیکال آزاد مورد ارزیابی قرارگرفت. تیمارها شامل گروه های کنترل، پوشش ژلاتین، کیتوزان، ژلاتین+اسانس، کیتوزان+اسانس و ژلاتین-کیتوزان+اسانس بودند. نمونه ها پس از آماده سازی در کیسه های استریل پلی اتیلنی در شرایط آزمایشگاه بسته بندی و 12روز در4 درجه نگهداری و به فاصله 3 روز مورد ارزیابی میکروبی، شیمیایی و حسی قرار گرفتند. نتایج آنالیز اسانس نشان داد که بیشترین ترکیبات تشکیل دهنده اسانس شامل اکالیپتول(24/51 درصد) و کامفور(22/02 درصد) می باشند. حداقل غلظت مهارکنندگی و کشندگی نانوامولسیون اسانس برازمبل به ترتیب 0/0625 و 0/125 درصد گزارش گردید. میزان بازهای ازته ی فرار و اندیس پراکسید در طول دوره ی مطالعه روند افزایشی داشته است. طبق نتایج حاصل از اندازه گیری شاخص پراکسید و pH، بین تمام تیمار ها و تیمار کنترل اختلاف معنی داری وجود داشت(0/05>P). میانگین لگاریتم کاهش باکتری در بین گروه ها تفاوت معنی داری داشت و بیشترین اثر ضد باکتریایی در پوشش کیتوزان-ژلاتین حاوی نانوامولسیون اسانس برازمبل مشاهده شد. بر اساس نتایج بدست آمده مشخص گردید که پوشش خوراکی دولایه ژلاتین-کیتوزان حاوی نانوامولسیون اسانس برازمبل دارای اثر ضدمیکروبی مؤثری بر علیه باکتری آئروموناس هیدروفیلا داشته و سبب حفظ ویژگی های حسی و افزایش ماندگاری ماهی قزل آلای رنگین کمان شده و می تواند پوشش مناسبی جهت افزایش عمر ماندگاری محصولات غذایی باشد.
کلیدواژه‌ها
آئروموناس هیدروفیلا
کیتوزان
ژلاتین
نانوامولسیون
برازمبل
زمان ماندگاری
ماهی قزل آلا
پوشش
بسیه بندی

موضوعات

عنوان مقاله English

Evaluation of antibacterial and antioxidant effect of gelatin-chitosan bilayer edible coating containing nanoemulsion of Perovskia abrotanoides Kar. essential oil on growth control of Aeromonas hydrophila inoculated into rainbow trout fillet

نویسندگان English

Katayoun Ahmadi
Mohammad Mohsenzadeh
Department of food hygiene and Aquaculture, Ferdowsi university of Mashhad
چکیده English

In this study, the effect of bilayer edible coating of gelatin-chitosan containing nanoemulsion of Perovskia abrotanoides Kar. essential oil on growth control of Aeromonas hydrophila inoculated into rainbow trout fillets over a 12-day period at 4 °C was investigated. The chemical composition of the essential oil was evaluated by GC-MS, its antibacterial properties by disk diffusion, plate well and micro-dilution broth, and its antioxidant properties by DPPH.Treatments included control, gelatin, chitosan, gelatin+essential oil, chitosan+essential oil and gelatin-chitosan+essential oil. After preparation, the treatments were packed in sterile polyethylene bags in laboratory conditions and stored for 12 days at 4 °C and their microbial, chemical and sensorial properties were evaluated every 3 days. The results of GC-MS showed that eucalyptol (24.51%) and camphor (22.02%) are the main components. The MIC and MBC of Perovskia abrotanoides Kar. essential oil nanoemulsions were reported to be 0.0625 and 0.125%, respectively. The amount of TVB-N and POV increased during the study period. According to the results of POV and pH measurements, there was a significant difference between all treatments and control treatment (P<0.05). The mean reduction log of bacteria was significantly different between all treatments and the highest antibacterial effect was observed in the gelatin-chitosan coating containing nanoemulsion of Perovskia abrotanoides Kar. essential oil. According to the results, Perovskia abrotanoides Kar. essential oil has good antioxidant and antimicrobial properties and according to the test results, it was found that chitosan-gelatin coating containing nanoemulsion of Perovskia abrotanoides Kar. essential oil has an effective antimicrobial effect against Aeromonas hydrophila and can protect sensory properties and increase shelf life of rainbow trout fillets as well as useful coating to increase the shelf life of food products.

کلیدواژه‌ها English

Aeromonas hydrophila
Chitosan
Gelatin
Rainbow trout
Nanoemulsion
Perovskia abrotanoides
shelf life
packaging
Coating
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