تولید نانوکپسول های حامل آستاگزانتین استخراج شده از میکروجلبک هماتوکوکوس (Haematococcus pluvialis) با پوشش ترکیبی مالتودکسترین-کازئینات سدیم و ارزیابی خواص فیزیکی، آنتی اکسیدانی و رنگی محصول

نویسندگان
سکینه یگانه 1
سهیل ریحانی پول 2
1 استاد، گروه شیلات، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
2 دانش آموخته دکتری تخصصی، گروه فراوری محصولات شیلاتی، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
10.22034/FSCT.19.131.303
چکیده
هدف تحقیق حاضر در مرحله اول استخراج آستاگزانتین از میکروجلبک هماتوکوکوس (Haematococcus pluvialis) با روش ترکیبی اسید-استون و ارزیابی بازده فرایند بود. سپس آستاگزانتین مستخرج با پوشش ترکیبی مالتودکسترین-کازئینات سدیم نانو ریزپوشانی و خواص فیزیکی، آنتی­اکسیدانی و رنگی نانوکپسول­ها در کنار فرم خالص رنگدانه (طی یک ماه نگهداری در دمای یخچال) ارزیابی شد. نتایج نشان داد با صابونی­کردن و خالص­سازی اولیه و ثانویه، بازده فرایند استخراج ارتقا و میزان رنگدانه طی مراحل مذکور، از 11/8 به 76/21 میلی­گرم بر گرم افزایش یافت. مطابق یافته­ها، سایز و پتانسیل زتای نانوکپسول­های تولیدشده به ترتیب 1/269 نانومتر و 71/46+ میلی­ولت بود؛ ضمن اینکه بازده فرایند ریزپوشانی 19/85 درصد ثبت شد. پروفیل رهایش آستاگزانتین از نانوکپسول­ها در محیط مشابه معده (SGF) و روده (SIF) نشان داد که رهایش رنگدانه در SGF از 21/3 تا 28/14 و در SIF از 49/18 تا 89/41 درصد متغیر است. بر اساس نتایج، فعالیت مهار رادیکال آزاد DPPH رنگدانه خالص و نانوکپسول­ها (در غلظت­های 100 و 200 میکروگرم بر میلی­لیتر و روزهای صفر، 15 و 30) به ترتیب از حدود 21 تا 57 و 53 تا 70 درصد متغیر بود. این میزان برای قدرت کاهندگی یون فریک به ترتیب از جذب 12/0 تا 54/0 و 55/0 تا 71/0 در طول موج 700 نانومتر گزارش شد. در آزمون فعالیت کلاته­کنندگی فلزات، دامنه تغییرات به ترتیب از 23 تا 52 و 52 تا 75 درصد ثبت گردید. با نانو ریزپوشانی رنگدانه و افزایش غلظت، فعالیت آنتی­اکسیدانی آن به صورت معنی­داری افزایش یافت (05/0>p)؛ همچنین برخلاف فرم خالص آستاگزانتین، فعالیت آنتی­اکسیدانی نانوکپسول­های حامل آن در طول زمان نگهداری، ثابت ماند (05/0<p). ارزیابی رنگ محصول نشان داد که شاخص روشنایی (L*) در نانوکپسول­ها به صورت معنی­داری بیشتر از فرم خالص رنگدانه است (05/0>p)؛ ضمن اینکه شاخص­های رنگی نانوکپسول­ها (بر خلاف آستاگزانتین خالص) با گذشت زمان، تغییر نکردند (05/0<p).
کلیدواژه‌ها
میکروجلبک هماتوکوکوس
آستاگزانتین
نانوکپسول‌های حامل
مالتودکسترین
خواص فیزیکی
فعالیت آنتی‌اکسیدانی

موضوعات

عنوان مقاله English

Production of nanocapsules carrying astaxanthin extracted from Haematococcus pluvialis with maltodextrin-sodium caseinate combined coating and evaluation of physical, antioxidant and color properties of the product

نویسندگان English

Sakineh Yeganeh 1
Soheyl Reyhani Poul 2
1 Professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 PhD graduate, Department of Processing of Fishery Products, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده English

The aim of the present research in the first stage was to extract astaxanthin from Haematococcus microalgae (Haematococcus pluvialis) by combined acid-acetone method and evaluate the efficiency of the process. Then, astaxanthin extracted was nanoencapsulated with maltodextrin-sodium caseinate combined coating and physical, antioxidant and color properties of nanocapsules were evaluated along with the pure form of the pigment (during one month of storage at refrigerator temperature). The results showed that with saponification and primary and secondary purification, the efficiency of the extraction process increased and the amount of pigment during the mentioned steps increased from 8.11 to 21.76 mg/g. According to the findings, the size and zeta potential of the produced nanocapsules were 269.1 nm and +46.71 mV, respectively; In addition, the efficiency of the nanoencapsulation process was recorded as 85.19%. The release profile of astaxanthin from nanocapsules in Simulated Gastric Fluid (SGF) and Simulated Intestinal Fluid (SIF) showed that the release of the pigment varies from 3.21 to 14.28 in SGF and from 18.49 to 41.89% in SIF. Based on the results, the DPPH free radical scavenging activity of pure pigment and nanocapsules (at concentrations of 100 and 200 μg/ml and 0, 15 and 30 days) was ranged from about 21 to 57 and 53 to 70%, respectively. This amount for the reduction power of ferric ion was reported from 0.12 to 0.54 and 0.55 to 0.71 absorbance at 700 nm wavelength, respectively. In the metal chelating activity test, the range of changes was recorded from 23 to 52 and 52 to 75%, respectively. With the nanoencapsulation of the pigment and increasing the concentration, its antioxidant activity increased significantly (p<0.05). Also, unlike the pure form of astaxanthin, the antioxidant activity of its carrier nanocapsules remained constant during storage (p>0.05).

کلیدواژه‌ها English

Haematococcus microalgae
astaxanthin
Carrier nanocapsules
Maltodextrin
Physical properties
Antioxidant activity
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